Prag1 (NM_001107315) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pragmin; RGD1311793
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209577 representing NM_001107315 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGTGCAGTGACTTTGTGGAGCACATCTGGAAACCCGGATCCTGCAAGAACTGCTTCTGCTTGA GGAGTGACCATCAGCTCACAGCTGGCCACCCCAAAGCTAGAGCCAGCAGCCTACCCGCTGGAGCTCGCCT GCCTGCCAGGCCTGAGATTTGCCGCTTAGAGGATGAAGGTGTGAATGGCTTGGCCTACTCAAAGCCCACC ATTGCGGTAAAGCCCACCATGATGACTTCTGAGACTGCCGACTTGTGGACTGAGGCCAGCCTGAGCGCTG AAGTTCCAAAGGTCAACTGGAGACGAACTCCAGGCAAGCTCCTGCTCCAGAAACAGGAAGATGGGCCAAT AGTTTACCTCGGCAGCTTCCGAGGAATGCAGAAGGCTGCAGGCCCTCTCGCCTGCACAGATAGCAACTCT CGTTGTCCCCCAGCCTACACCATGGTCGGACTGCATAACCTGGAGGCCCGAGTGGATAGGAACACCGCCC TCCAACCAGTGAATTTCCAGGAAGAGAAGGCTGGGAGGGAAGAGCTGCCCTCAGCTCAGGAGAGCTTTCG TCAGAAGCTAGCTGCCTTTACAGGGATGACGTCCAGCTGCCTCAAGGGCCCCAGACCCTGCACTTCTCCA CAGCCCTTGCGGGAGTCCCTGCCCTCAGAGGATGACAGTGACCAAAGGTGCTCACCCTCAGGAGACAGCG AAGGTGGGGAGTACTGCTCCATTTTGGACTGTCGTCCAGAATCCAGAGATGCTGTGCATAACACCGAGGG CTCTGGCCGACGGCGAGGGGACTGCTCACCCATATGCTGGGAGCAGGGAACATGTACAAGACCAACAGAA GAGGAAAAGCAGGCTCTGAACTTCCCCAGAGAGTGTTGTGGTCAGGGATCCACAGCAAACCCACCCCACC TGGGCCCCAAGAAGCCGTCCCTTAACTCAGAGGCTGCCAGCTCTTCAGATGGTCTGTCCTGTGGCAGCAG CCGCAGTGGTGCCAACAGCCCCTTTGCTCCACACCTGGAGAATGACTACTGCTCTCTTGTGAAGGAGCCA ACCTCGGTGAAGCAGCAGGACTCTGGCTGCCACTTAGTCAACTCTGGCAAATATGTGGGTCAAGCCGTGG ACCTCCAGCCCCCAGCCCTTCCTAGGGAGGCTGTGCAGCCTGAACCCATCTACGCCGAAAGTGCCAAGAG GAAGAAGGCAGCTCCGGTGCCTCAGAGGCCCGAGCCCAAGAAAGAGCAGGTCTCGTCTGGCCAAGTATGG ACAGGCGATACCTGGTCTCAGAAGACACCCTCTGGCTGGAGCCAGGAAAGGGAAGGCCCAAACGCTGCTC CTCAGGTGGCAACCACTATTACTGTTATTGCTGCTCACCCAGAAGAGGACCATAGGACTATTTATCTGAG CAGCCCAGACTCTGCCGTGGGGGTGCAGTGGCCACGTGGGTCTTTGAACCAGGACTTACATGGTAGTGGA GAAGAGCCTTTGGTCGTGCAGGGGCTAAGCTCAAGGGAGAGCCATCCACATAATATGACAGAAAACTCGT CCAAAGAAAAGCCTGCCATACCCCCCAAGCTGTCCAAAAGCAGCCCGGGAGGGTCCCCGGTGTCACCTGC AGCCCCCCCTCTGACTGACCACAGTGATGGGAACACCAGTGGCAGCAGTGTCGGATCTCAGCCTTCGTCT AGAGTCCCTACTAACCTAACTTCTTCCTGCCAGACCAATGGTGTTGCCGCCGGTGACCCTGCCAAGTGTC CCCCACAAGCCAACTCTTCAGTCTTGGACCAGAGGCGGCCAAGATACCAGACTGGTGCATGGAGCCGCCA GTGCCGGATAGAGGAGGAGGAAGAGGTGGGGCAGGAGTTGTTGAGTCAAAGTTGGGGGAGAGAGTTGGAA AATGGTATTGCAGACCATTCAAACTCCTCTACCTGGCACCGGCTCCATCCCATTGATGGCGCCTCTGGGC AGAACGGCAAAACTAACTCTGGGATGAGCAAATCAGCTTCCTTTGCGTTTGAGTTCCCAAAGGACAGAGG CAGATTGGAGTCATTCTCACCCCCTCCCCCACCTCCAAAGTCAAGGCACCTGTTAAAAATGAACAAGAGC AGCTCTGACTTGGAGAAAGTGAGCCAGAGCTCTGCAGAAAGCCTCAGCCCGTCCTTCCGAGGGGCCCACG TCAGCTTCACCACGGGCTCCACAGATAGCCTAGCCTCGGACTCCAGGACTTGCAGCGATGGAGGTCCATC GTGTGAAGCCACCCACTCACCCACCATCAGCGGGAAAAAGCTCTTTGCTCCCGTCCCTTTCCCGTCAGGC TCCACGGAGGATGTGTCCCCCAGCGGTCCTGCACAGCCTCCTCCTCTGCCCCAGAAAAAGATAGTGAGCC GTGCTGCCTCCTCCCCTGATGGCTTCTTCTGGACCCAAGGTTCCCCTAAGCCACGCACAGCGAGTCCCAA GCTGAACCTAAGCCACTCGGAAACCAATGTTTGTGCCCATGACGAGCCTCCTTTGAGCTATTCCTTGAAC TCTGGAAACCATCCCCACCATGTCTTCTCCTCTTCTGAGCCTTTGGAGAAAGCTTTCAAAGGCAGTGTCC CCTGGGCCCCAGCTCTGGGTCCAGCAAACAGCAAAGGCGGTTGTGGGAGCCCCAATCTCCAGGGCAGAGC GGCCACCTCCACCTCGTCATCCCAGCTCAGCGTGTCCAGCCAGGCCTCCACCGGCAGCAGCCAGCTTCAG CTGCACAGCCTGCTGAGCAGTATCAGCAGCAAGGAGGGCACCTATGCCAAGCTCGGGGGCCTCTACACAC AGTCCCTGGCCCGCCTAGTGACCAAGTGCGAAGACCTCTTCATGGGTGGTCTGAAAACGGAACTGCGCTT CGATGAGAACAGCTGGTCGCTCTTCAAACTGATATGCAACAAGCCTTGCTGTGACTCGGGAGATGCAATT TACTACGGTGCCACTTGCTCCAAGGACCCCGACAGCATCTACGCAGTGAAAATCTGCAAAACCCCAGAAC CCAAGTCAGCTTCGTACTGTAGCCCGTCCGTGCCAGTGCACTTCAACATCCAGCAAGACTGCGGCCACTT TGTGGCCTCTGTGCCCTCCAGCATGCTTGCCTTCCCCGACACATCCAGCAAGGACCCTGCGCCTGCTGCG CCCTCACACACCCCTGCCCAGGAGCAGGACTGTGTGGTGGTCATCACCCGGGAGGTGCCGCACCAGACTG CCTCGGACTTTGTGCGGGACTCTGTGGCCAGCCACCGCGCAGAGCCTGAGGTTTACGAGCGCCGGGTGTG CTTCTTGCTTCTGCAGCTCTGCAACGGGCTGGAGCATCTGAAGGAACATGGGATCATCCACCGGGACCTA TGTCTGGAGAACCTGTTGCTAGTGCATTGCAACCCTCAGAGCTCTCCCGGGCCCTCTGCTAATCCCAGCG TGCCCACCACCACGTCCCGTTGTCCTTCTGCCGCACCCGCAGCCACCACAGCCTGCCAGGGAGGACCTGG TGAGAAGCACCTGCCCAGGCTGATCATCAGCAACTTCCTGAAGGCCAAGCAGAAGCCGGGAGGCACCACC AACCTGCAACAGAAGAAGAGCCAGGCCCGGCTGGCCCCTGAGATCGTATCTGCCTCTCAGTACCGTAAGT TTGATGAGTTCCAGACTGGCATCCTCATCTACGAGCTGCTCCACCAGCCCAACCCATTCGAGGTGCGAGC CCAGCTACGGGAGCGTGACTACCGGCGGGAGGACCTACCCCCGCTGCCCACACTGTCGCTCTACTCGCCC GGCCTGCAGCAGCTCGCCCACCTGCTCCTTGAGGCCGATCCCATCAAGCGCATCCGCATAGGTGAGGCCA AGCGGGTGCTACAGTGCTTGCTGTGGGGGCCACGGCGAGAGCTGGTAGAGCAGCCGTGCCCCTCGGAGGA GGTACTGTGCAACACTCTGCACAACTGGATCGACATGAAGCGAGCCCTGATGATGATGAAGTTTGCCGAG AAGGCGGTAGAACGAAGGCGGGGTGTGGAGCTGGAGGATTGGCTCTGTTGCCAGTACTTGGCCTCAGCAG AGCCAGGGGCCCTCTTGCAGTCCCTGAAGCTCCTGCAACTTCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107315
Insert Size	4107 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107315.1, NP_001100785.1
RefSeq Size	4759 bp
RefSeq ORF	4107 bp
Locus ID	306506
UniProt ID	D3ZMK9
Gene Summary	Catalytically inactive protein kinase that acts as a scaffold protein (PubMed:29503074). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (PubMed:16481321). Promotes Src family kinase (SFK) signallig by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (PubMed:27116701, PubMed:21873224). Acts as a critical coactivator of Notch signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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