Prag1 (NM_001107315) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pragmin; RGD1311793
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209577 representing NM_001107315 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGTGCAGTGACTTTGTGGAGCACATCTGGAAACCCGGATCCTGCAAGAACTGCTTCTGCTTGA GGAGTGACCATCAGCTCACAGCTGGCCACCCCAAAGCTAGAGCCAGCAGCCTACCCGCTGGAGCTCGCCT GCCTGCCAGGCCTGAGATTTGCCGCTTAGAGGATGAAGGTGTGAATGGCTTGGCCTACTCAAAGCCCACC ATTGCGGTAAAGCCCACCATGATGACTTCTGAGACTGCCGACTTGTGGACTGAGGCCAGCCTGAGCGCTG AAGTTCCAAAGGTCAACTGGAGACGAACTCCAGGCAAGCTCCTGCTCCAGAAACAGGAAGATGGGCCAAT AGTTTACCTCGGCAGCTTCCGAGGAATGCAGAAGGCTGCAGGCCCTCTCGCCTGCACAGATAGCAACTCT CGTTGTCCCCCAGCCTACACCATGGTCGGACTGCATAACCTGGAGGCCCGAGTGGATAGGAACACCGCCC TCCAACCAGTGAATTTCCAGGAAGAGAAGGCTGGGAGGGAAGAGCTGCCCTCAGCTCAGGAGAGCTTTCG TCAGAAGCTAGCTGCCTTTACAGGGATGACGTCCAGCTGCCTCAAGGGCCCCAGACCCTGCACTTCTCCA CAGCCCTTGCGGGAGTCCCTGCCCTCAGAGGATGACAGTGACCAAAGGTGCTCACCCTCAGGAGACAGCG AAGGTGGGGAGTACTGCTCCATTTTGGACTGTCGTCCAGAATCCAGAGATGCTGTGCATAACACCGAGGG CTCTGGCCGACGGCGAGGGGACTGCTCACCCATATGCTGGGAGCAGGGAACATGTACAAGACCAACAGAA GAGGAAAAGCAGGCTCTGAACTTCCCCAGAGAGTGTTGTGGTCAGGGATCCACAGCAAACCCACCCCACC TGGGCCCCAAGAAGCCGTCCCTTAACTCAGAGGCTGCCAGCTCTTCAGATGGTCTGTCCTGTGGCAGCAG CCGCAGTGGTGCCAACAGCCCCTTTGCTCCACACCTGGAGAATGACTACTGCTCTCTTGTGAAGGAGCCA ACCTCGGTGAAGCAGCAGGACTCTGGCTGCCACTTAGTCAACTCTGGCAAATATGTGGGTCAAGCCGTGG ACCTCCAGCCCCCAGCCCTTCCTAGGGAGGCTGTGCAGCCTGAACCCATCTACGCCGAAAGTGCCAAGAG GAAGAAGGCAGCTCCGGTGCCTCAGAGGCCCGAGCCCAAGAAAGAGCAGGTCTCGTCTGGCCAAGTATGG ACAGGCGATACCTGGTCTCAGAAGACACCCTCTGGCTGGAGCCAGGAAAGGGAAGGCCCAAACGCTGCTC CTCAGGTGGCAACCACTATTACTGTTATTGCTGCTCACCCAGAAGAGGACCATAGGACTATTTATCTGAG CAGCCCAGACTCTGCCGTGGGGGTGCAGTGGCCACGTGGGTCTTTGAACCAGGACTTACATGGTAGTGGA GAAGAGCCTTTGGTCGTGCAGGGGCTAAGCTCAAGGGAGAGCCATCCACATAATATGACAGAAAACTCGT CCAAAGAAAAGCCTGCCATACCCCCCAAGCTGTCCAAAAGCAGCCCGGGAGGGTCCCCGGTGTCACCTGC AGCCCCCCCTCTGACTGACCACAGTGATGGGAACACCAGTGGCAGCAGTGTCGGATCTCAGCCTTCGTCT AGAGTCCCTACTAACCTAACTTCTTCCTGCCAGACCAATGGTGTTGCCGCCGGTGACCCTGCCAAGTGTC CCCCACAAGCCAACTCTTCAGTCTTGGACCAGAGGCGGCCAAGATACCAGACTGGTGCATGGAGCCGCCA GTGCCGGATAGAGGAGGAGGAAGAGGTGGGGCAGGAGTTGTTGAGTCAAAGTTGGGGGAGAGAGTTGGAA AATGGTATTGCAGACCATTCAAACTCCTCTACCTGGCACCGGCTCCATCCCATTGATGGCGCCTCTGGGC AGAACGGCAAAACTAACTCTGGGATGAGCAAATCAGCTTCCTTTGCGTTTGAGTTCCCAAAGGACAGAGG CAGATTGGAGTCATTCTCACCCCCTCCCCCACCTCCAAAGTCAAGGCACCTGTTAAAAATGAACAAGAGC AGCTCTGACTTGGAGAAAGTGAGCCAGAGCTCTGCAGAAAGCCTCAGCCCGTCCTTCCGAGGGGCCCACG TCAGCTTCACCACGGGCTCCACAGATAGCCTAGCCTCGGACTCCAGGACTTGCAGCGATGGAGGTCCATC GTGTGAAGCCACCCACTCACCCACCATCAGCGGGAAAAAGCTCTTTGCTCCCGTCCCTTTCCCGTCAGGC TCCACGGAGGATGTGTCCCCCAGCGGTCCTGCACAGCCTCCTCCTCTGCCCCAGAAAAAGATAGTGAGCC GTGCTGCCTCCTCCCCTGATGGCTTCTTCTGGACCCAAGGTTCCCCTAAGCCACGCACAGCGAGTCCCAA GCTGAACCTAAGCCACTCGGAAACCAATGTTTGTGCCCATGACGAGCCTCCTTTGAGCTATTCCTTGAAC TCTGGAAACCATCCCCACCATGTCTTCTCCTCTTCTGAGCCTTTGGAGAAAGCTTTCAAAGGCAGTGTCC CCTGGGCCCCAGCTCTGGGTCCAGCAAACAGCAAAGGCGGTTGTGGGAGCCCCAATCTCCAGGGCAGAGC GGCCACCTCCACCTCGTCATCCCAGCTCAGCGTGTCCAGCCAGGCCTCCACCGGCAGCAGCCAGCTTCAG CTGCACAGCCTGCTGAGCAGTATCAGCAGCAAGGAGGGCACCTATGCCAAGCTCGGGGGCCTCTACACAC AGTCCCTGGCCCGCCTAGTGACCAAGTGCGAAGACCTCTTCATGGGTGGTCTGAAAACGGAACTGCGCTT CGATGAGAACAGCTGGTCGCTCTTCAAACTGATATGCAACAAGCCTTGCTGTGACTCGGGAGATGCAATT TACTACGGTGCCACTTGCTCCAAGGACCCCGACAGCATCTACGCAGTGAAAATCTGCAAAACCCCAGAAC CCAAGTCAGCTTCGTACTGTAGCCCGTCCGTGCCAGTGCACTTCAACATCCAGCAAGACTGCGGCCACTT TGTGGCCTCTGTGCCCTCCAGCATGCTTGCCTTCCCCGACACATCCAGCAAGGACCCTGCGCCTGCTGCG CCCTCACACACCCCTGCCCAGGAGCAGGACTGTGTGGTGGTCATCACCCGGGAGGTGCCGCACCAGACTG CCTCGGACTTTGTGCGGGACTCTGTGGCCAGCCACCGCGCAGAGCCTGAGGTTTACGAGCGCCGGGTGTG CTTCTTGCTTCTGCAGCTCTGCAACGGGCTGGAGCATCTGAAGGAACATGGGATCATCCACCGGGACCTA TGTCTGGAGAACCTGTTGCTAGTGCATTGCAACCCTCAGAGCTCTCCCGGGCCCTCTGCTAATCCCAGCG TGCCCACCACCACGTCCCGTTGTCCTTCTGCCGCACCCGCAGCCACCACAGCCTGCCAGGGAGGACCTGG TGAGAAGCACCTGCCCAGGCTGATCATCAGCAACTTCCTGAAGGCCAAGCAGAAGCCGGGAGGCACCACC AACCTGCAACAGAAGAAGAGCCAGGCCCGGCTGGCCCCTGAGATCGTATCTGCCTCTCAGTACCGTAAGT TTGATGAGTTCCAGACTGGCATCCTCATCTACGAGCTGCTCCACCAGCCCAACCCATTCGAGGTGCGAGC CCAGCTACGGGAGCGTGACTACCGGCGGGAGGACCTACCCCCGCTGCCCACACTGTCGCTCTACTCGCCC GGCCTGCAGCAGCTCGCCCACCTGCTCCTTGAGGCCGATCCCATCAAGCGCATCCGCATAGGTGAGGCCA AGCGGGTGCTACAGTGCTTGCTGTGGGGGCCACGGCGAGAGCTGGTAGAGCAGCCGTGCCCCTCGGAGGA GGTACTGTGCAACACTCTGCACAACTGGATCGACATGAAGCGAGCCCTGATGATGATGAAGTTTGCCGAG AAGGCGGTAGAACGAAGGCGGGGTGTGGAGCTGGAGGATTGGCTCTGTTGCCAGTACTTGGCCTCAGCAG AGCCAGGGGCCCTCTTGCAGTCCCTGAAGCTCCTGCAACTTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209577 representing NM_001107315 Red=Cloning site Green=Tags(s) MSACSDFVEHIWKPGSCKNCFCLRSDHQLTAGHPKARASSLPAGARLPARPEICRLEDEGVNGLAYSKPT IAVKPTMMTSETADLWTEASLSAEVPKVNWRRTPGKLLLQKQEDGPIVYLGSFRGMQKAAGPLACTDSNS RCPPAYTMVGLHNLEARVDRNTALQPVNFQEEKAGREELPSAQESFRQKLAAFTGMTSSCLKGPRPCTSP QPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCRPESRDAVHNTEGSGRRRGDCSPICWEQGTCTRPTE EEKQALNFPRECCGQGSTANPPHLGPKKPSLNSEAASSSDGLSCGSSRSGANSPFAPHLENDYCSLVKEP TSVKQQDSGCHLVNSGKYVGQAVDLQPPALPREAVQPEPIYAESAKRKKAAPVPQRPEPKKEQVSSGQVW TGDTWSQKTPSGWSQEREGPNAAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGSLNQDLHGSG EEPLVVQGLSSRESHPHNMTENSSKEKPAIPPKLSKSSPGGSPVSPAAPPLTDHSDGNTSGSSVGSQPSS RVPTNLTSSCQTNGVAAGDPAKCPPQANSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELLSQSWGRELE NGIADHSNSSTWHRLHPIDGASGQNGKTNSGMSKSASFAFEFPKDRGRLESFSPPPPPPKSRHLLKMNKS SSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRTCSDGGPSCEATHSPTISGKKLFAPVPFPSG STEDVSPSGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPLSYSLN SGNHPHHVFSSSEPLEKAFKGSVPWAPALGPANSKGGCGSPNLQGRAATSTSSSQLSVSSQASTGSSQLQ LHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGLKTELRFDENSWSLFKLICNKPCCDSGDAI YYGATCSKDPDSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLAFPDTSSKDPAPAA PSHTPAQEQDCVVVITREVPHQTASDFVRDSVASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDL CLENLLLVHCNPQSSPGPSANPSVPTTTSRCPSAAPAATTACQGGPGEKHLPRLIISNFLKAKQKPGGTT NLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSP GLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCPSEEVLCNTLHNWIDMKRALMMMKFAE KAVERRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107315
ORF Size	4104 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107315.1, NP_001100785.1
RefSeq Size	4759 bp
RefSeq ORF	4107 bp
Locus ID	306506
UniProt ID	D3ZMK9
MW	147.7 kDa
Gene Summary	Catalytically inactive protein kinase that acts as a scaffold protein (PubMed:29503074). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (PubMed:16481321). Promotes Src family kinase (SFK) signallig by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (PubMed:27116701, PubMed:21873224). Acts as a critical coactivator of Notch signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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