Mlh3 (NM_001108043) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209710 representing NM_001108043 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGGTGTCTGTCAGATGAAGTACAAGCCAAGTTGCGTTCTGGTTTAGCCCTAAGCTCCTTAGGCC AGTGTGTTGAGGAACTTACCCTTAACAGTATTGATGCTGAGGCAACATGTGTGGCCATCAGAGTGAATAT GGAAACCTTCCAAGTTCAAGTGATAGACAACGGACTTGGGATGGCGGGGGATGACGTGGAGAAGGTGGGG AACCGGTATTTTACTAGTAAATGCCACTCAGTACGGGACCTGGAGAACCCAACGTTTTACGGTTTCCGAG GCGAGGCCTTGGCAAGTATAGCTGACATGGCTAGTGCTGTGGAAATTTCATCCAAGAAAAGCACAACATT GAAGACCTTTGTGAAGATGTTTCAGAACGGAAAAGCCCTCGCCGCCCGTGAGGCGGACTTGACCAGACCG AGTGTGGGGACTACAGTGACGGTCTATAACCTGTTTTACCAGTTTCCTGTGCGGAGGAAAAGCATGGACC CTAGACTAGAGTTTGAGAAGGTTAGGCAGAGGGTGGAAGCCCTCTCACTTATGCACCCCTCCATTTCTTT CTCTTTGAGGAATGACGTTTCTGGTTCCATGATTCTTCAGCTCCCTAAAACCAAAGACATATGTTCTCGG TTTTGTCAAATTTATGGATTGGGCAAGTCCCAAAAGTTAAGAGAAATACATTATAAGTATAAGGAATTTG AGTTTAATGGCTACATCAGCTCTGAAGCACACTACAATAAGAATATGCAGTTTTTGTTTGTGAACAGAAG ACTAGTTTTAAGAACAAAGTTGCATAAACTTATTGACTTTTTATTAAGGAAAGAAAGTATTATATGCAGG CCAAAGAATGGCTCTGCCAGTAGGCAAATGAATTCAAGTCCTCGACACCGTTCTGCCTCAGAACTCCATG GGATATATGTAATCAACGTGCAGTGCCCGTTCTGTGAGTATGATGTCTGCATAGAGCCAGCCAAAACTCT GATTGAATTCCAGAACTGGGATACCGTGTTGATTTGTGTTCAGGAAGGAATAAAAAAGTTTTTAAAGCAA GAAAAATTATTTGTAGAATTATCAAGTGAAGATATTAAGGAATTTAATGAAGATAATGGTTTTAGTTTGA TTGGTACAACACTTCAGACACAGTCTACTCATGAGAAGTGTGACCAGAGCAGTTTCCAGGAAGCGTGTAA TAATATTTTGGATTCCTATGAAATGTTTAATTTGCAGTCAAAAGCTGTGAAAAGAATAGCTACTTTAGAA AATAGAACCACACAAAATTCTGGCGATTCAGGAGCTATCAGAAAAAAGACAGAGGACTCATACACTTATG AATCAGATGACCTGGGCCATAGAAAAACTGTAGAGTCTGCGTTATACAACAGTGACAGTGCTTACTTAGG ATCAAGGATGTCAGAAGAAGACATAGCCAAAACATCACACGCTGGAGAAAATGAGAAATGGAAAAAATCT TTGTTGGAAAATAGGACTTCAGAAAGGCCACATGAAACCAGTCCAAAAATGTTTTCAAGCCCCATTCAAA CTCATCATCCCCTTGAGGAGAGTGAGGCAGATCTAGAAATAGAGATGAAAAGTAGTACTGTTAATGTCAT GGCTGCCAACATTCCTCCAAATAGTGAAATTCAGAGTCAACTGGAGCAACTTGAAGATGCTCCTGAAGTG GAGTGCCAACCTCTGCCCTTTGAGACAACATTATTAAGAGCACAGGGTACTCTGAAAAAGAAGGAGAGAA AAAAGGAGCTCAGTAGCCATGGAAGAGTAAATGTTTTTAGTTATGGACAGGTTAAATTGTGCTCCACTGG CTTTATCACTCATGTGGTACAACGCGAACCCGCCAAGTCACCTGAAACAGAACAGTTGTTTAAAAATTAT GCTCGACCTGGTCCTGTAAGTGCCCAGGAAGCATTTGGAAATAGAACACACCATGCAAGTGAGACTCCAG ACAGCAGTAGTTTAACAAGCACTTTAAGTAAAGAATCCACTCAGCCGCCCCACAAAAGATTCTGCAGAAC AAATACAGGTTATGGGACAGAGAACAAACCTGTAGCAACAGATGACAACTTGGCCCTTTTTCAGGGAAGC CGTAAAGAATCACACACAGATTGCTTTTTGCCTGATACAACCTCCTTTCCGTGGTGTAGAAATGTTTCCG GTGGTTATAAGAAAAAAGGTAAAGTGGGTTCCTTCAAACACGTAGTCCGTAGGAAGCTAAGCTTGCGTTC ACAAATAGGGTCTTTAGAGAAGTTTAAGAGGCAATATGGGAAGGTTAACAATTCCCTAGCTGCAGAAGAG GACAATGACACTGACTTCAGGACCCATCTTGAGCCTCACAATGAACCCGATGTTCTTCTGAAAGATAAGA GCCTCTCAGATATGTCTGATGGTGGTGAGATCACTCCATCAGAGCACAGTGATGGGTGTCAACCATTACG TCCCATCCTGTACCCAGAGACACTTTCCAAAGAAGATCGCTTAGAACAGAGGCCTAGTTTGAGAGAAAGT CCTATCACTCTGGCGGAATTGCTCCACGGTAACAGAAAACCTTCTGAGGTTAAGAAGTCCACTGAAGCCC TGGCTTCTAAATTATCCAGACTCAAGGATTCTGAAAAAGAGATGCAAACCATGGAGATGGCAGGTCCTGT TAGTGAACGTCCAGACTCAGATCCCAGTTGGAAAGACAATAGCCAGTGCGCTAGGCTGGACTTAAATTTT TGTGAATTATATAAAAATAAACTTGAAAAAACAGAGAGTGAAGTGCTTCCAATGCCAGATTCTGCCCCAG AGGATGATTCCATCAATAAAAACAGCGTGCTGTGTTCTAACAGTGTAACAGATGACAGGGAGAAACCAGA AACTTCCTTGCTGTTACCCTGTAGTGATTCTAAAATCAGCAAAGATTCAGATGTTCTTATCAGAGTCTCA GAGCAACAGAGTGGAAGCCCTGACTCTGCCAGCAGGGAGGTAACGCGTCAGGTAGAAGATAGCGCCGGCA GCCAATGTGGAATCTGTTCCCAGAGCCATGGGTTTAAAGCAAGATCTTGTTCTGACAATGAAGAATCAAG TACGCACAGTATGGACTGGCAGCAGCATTTTGACGTAACCCTGGGAAGAACGGTTTACATCAACAGAATG ACCGGGCTTAGCACGTTTGTTGCTCCTACTGATGACATTCAGACTCCTTGTACTAAAGATCTGACAACCG TGGCTGTGGATGTCCTACTTGGGAATGGGGTCCAGTACAGGTGTCATCCTTTTAGAAGCGACCTTGTTCT TCCTTTCCTCCCAAGAGCTCAGGAAGAGAGGACCAGGCGGAGACACAACAGAGATGCTGTAGATGCTGCT GCTGTCAGTGAACCACTTCAGTCTTTGTTTTCAGAATGGAGCAATCCAGTGTTTGCCCGATACCCAGAGG TTGCTGTTGACGTCAGCAGTGGCCAGGCTGGGAGCTTAGCCGTTAAGATTCACAATGTTCTATATCCCTA TCGCTTCACCAAAGAGATGATCCACTCAGTGCAGGTTCTCCAGCAAGTGGATAACAAGTTTATCGCCTGC TTAATGAGCTCGAGGACGGACGGGAGTGGCCAAGCAGGTGGAAACCTGTTAGTCCTGGTGGACCAGCACG CTGCCCATGAGCGCGTTCGTTTGGAGCAGCTAATCACTGATTCCTATGAGAAACAAGCTCCACAAAGTGC TGGCCGGAAGAAATTACTGTCTTCCACAATAATTCCTCCACTAGCGATCACCGTGTCAGAGGAACAAAGG AGACTCTTACGGTCTTACCACAAACATTTGGAAGATCTGGGACTTGAGTTGCTCTTTCCAGATGCTAGTG ACTCTTCGATCCTGGTGGGAAAAGTGCCACTCTGCTTTGTAGAAAGAGAAGCTAATGAACTTCGAAGAGG ACGATCTCCTGTGACTAAGAGTATTGTGGAGGAATTGATTCGAGAACAGCTGGAGCTGCTCCAGACCACG GGAGGTATCCAAGGGACACTGCCGCTGACTGTCCAGAAGGTGTTGGCCTCCCAGGCCTGCCACGGGGCCA TTAAGTTTAATGATCGTCTGAGTCTGGAGGAGAGCTGTCGGCTTATCGAAGCTCTGTCCTTGTGCCAGCT GCCATTTCAGTGTGCTCATGGGCGACCTTCAATGCTGCCCTTAGCTGACCTGGACCACTTGGAGCAGGAA AAACAGGTTAAACCCAATCTTACTAAACTTCGCAAAATGGCTCATGCCTGGCATCTCTTTGGAAAAGCAG AAGGCTGTGATACAAAGCAGGAGCCACAACAGCCCGTACCTCCTTGTGAGCCACCGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001108043
Insert Size	4329 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108043.1, NP_001101513.1
RefSeq Size	5424 bp
RefSeq ORF	4329 bp
Locus ID	314320

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