Ppfia1 (NM_001106320) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210272 representing NM_001106320 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAGGTGATGCCGACCATTAGTGAAGCGGAAGGACCCCCAGGAGGAGGTGGGAGCCATGGGT CAGGCTCCCCTTCCCAGCCAGACGCTGATTCGCATTTTGAGCAGCTCATGGTTTCCATGCTGGAAGAGCG AGACCGCCTTCTGGACACACTGAGAGAGACACAAGAAACGCTGGCATTAACCCAGGGGAAGTTGCATGAA GTTGGTCATGAAAGAGATTCTTTGCAGAGGCAGCTCAACACTGCACTTCCACAGGAGTTTGCTGCACTCA CGAAGGAACTCAATGTGTGTCGGGAGCAGCTTCTGGAGAGAGAGGAAGAAATTGCTGAGCTGAAGGCAGA GAGGAACAACACTAGGCTGCTGTTGGAGCACCTAGAGTGCCTTGTCTCCAGGCACGAGCGCTCTCTACGT ATGACAGTGGTGAAGAGGCAGGCACAGTCACCTGCGGGCGTGTCCAGTGAGGTGGAGGTTCTGAAAGCCC TGAAGTCACTGTTTGAGCACCACAAAGCCTTGGATGAGAAGGTACGAGAGCGATTACGAGTAGCTCTGGA GAGATGCAGCTTGTTAGAAGAGGAGCTAGGTGCCACCCACAAAGAGCTAATGATTCTTAAAGAACAGAAC AGTCAGAAGAAAGCACTAACAGAGGGAATGCTTGATGGGAACCACGAACAGGAAAATGCACCGAGCACCA ACGGCAAGAGATCTTCCGATGGCTCCTTGAGCCATGAGGATCTTGCCAAAGTGCTGGAGCTGCAGGAAGT CATAGACAGGCAGGCTCGAGAGCAGAGCCAGATGAAGGAGCGCCTGGCCTCCCTGTCCAGTCATGCAGCA GAGCTGGAAGAGGATCTGGACACAGCTAGAAAAGACCTCATCAAGTCTGAAGAGATGAATACGAAACTGC AGCGAGAAGTCCGAGAAGCAATGGCCCAGAAGGAGGATATGGAGGAGAGAATCACCACCCTTGAGAAACG CTACCTCGCCGCACAGCGTGAAGCCACGTCTGTGCATGACCTCAATGACAAACTTGAAAATGAAATTGCA AATAAAGACTCCATGCACCGACAGACAGAGGACAAGAACCGCCAGTTGCAAGAACGTCTGGAATTGGCAG AGCAGAAGCTGCAGCAGACCCTGCGCAAAGCTGAGACGCTGCCAGAGGTGGAGGCTGAGCTGGCCCAGAG GGTGGCTGCACTCTCCAAGGCTGAGGAGAGACATGGCAACATTGAAGAGAGGCTGCGGCAGATGGAGGCA CAGCTGGAGGAGAAGAATCAGGAGCTGCAGCGGGCAAGACAGAGAGAAAAGATGAATGAGGAACATAATA AGCGCCTGTCTGACACTGTGGACAAGTTGCTCTCAGAATCCAATGAGAGGCTGCAGCTGCATCTGAAGGA GAGAATGGCGGCGCTGGAGGACAAGAATTCTCTGCTAAGGGAAGTTGAAAATGCAAAGAAGCAACTAGAA GAGACCCAGCATGATAAGGACCAACTTGTCTTGAATATTGAAGCCCTGAAGGCTGAGCTGGAGCAGATGA GACTGAGAGGTTCTTCACTTCATCATGGCCGACCCCATTTGGGCAGTGTCCCGGACTTCAGGTTCTCTGT GGCAGACGGGCATGTGGATGCTTACAGCACCAGTGCAGTGTTGCGGCGCCCCCAGAAGGGCCGGCTGGCA GCGCTGCGGGACGAGCCCTCCAAGGTGCAGACCCTCAATGAACAGGACTGGGAACGGGCCCAACAAGCCA GCGTCTTGGCAAATGTGGCACAGGCATTTGAGAGTGATGTGGATGTGTCTGATGGTGAAGATGACCGAGA CACTCTGCTCAGCTCAGTTGATCTGCTGTCACCCAGTGGGCAAGCTGATGCCCAGACCCTGGCCATGATG CTTCAGGAACAGCTGGATGCGATCAACAAAGAGATCAGACTGATCCAAGAAGAGAAGGAGAATACAGAGC AGCGGGCAGAGGAGATCGAGAGCAGAGTGGGCAGTGGAAGCTTGGACAATCTTGGTCGTTTTAGATCAAT GAGCTCCATACCCCCCTACCCTGCTTCCTCGCTTGCCGGCTCCTCTCCTCCAGGCAGTGGTCGGTCCACA CCCAGAAGGGTCCCTCACAGTCCTGCCCGGGAGGTGGACAGGCTGGGTGTCATGACTCTGTTGCCAGCTT CCAGAGAAGAGGTACGAGACGACAAGACGACGATAAAATGTGAAACCTCCCCCCCATCCTCCCCAAGGCC TCTGCGCTTAGACAGGCTGCACAAGGGTGCGCTGCACACGGTCAGCCATGAGGACATCAGGGATCTGAGG AACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAACCCTAGCAGCAGCAATAGTAGCCAGGACTCCCTCC ACAAAGCCCCAAAGAAGAAGGGCATCAAATCCTCCATTGGCCGCTTATTTGGGAAGAAGGAAAAGGGCCG GCCTGGTCCGCTGGGCAAGGAGTCACCAGGGCAAGCTGGTGTTTCAGAGACAGAAAATTCATCTCAAGAT GCCTTGGGACTCAGCAAACTGGGAGGGCAAGCTGAGAAAAACCGGAAACTTCAGAAAAAGCATGAGCTGC TGGAGGAGGCCCGCAGGCAAGGCTTGCCTTTCGCACAGTGGGATGGGCCAACTGTGGTGGTGTGGCTAGA GCTCTGGGTTGGGATGCCTGCCTGGTATGTGGCTGCTTGCCGAGCAAACGTCAAGAGTGGTGCCATTATG TCAGCCCTGTCAGACACTGAGATCCAGCGGGAGATTGGCATCAGCAACCCCTTGCACAGGCTGAAGCTGC GGCTGGCTATCCAAGAGATAATGTCACTGACTAGCCCTTCTGCTCCACCCACATCTAGGACGACGCTAGC ATATGGGGACATGAACCACGAGTGGATTGGCAACGAGTGGCTGCCCAGCTTGGGCCTGCCGCAGTACCGC AGCTACTTCATGGAGTGCCTTGTCGATGCCCGGATGCTGGATCACCTGACCAAGAAAGACCTTCGAGGGC AGCTGAAGATGGTCGACAGCTTCCATAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGTTGAA TTATGATCGAAAAGAACTGGAAAGAAAACGAGAAGAGAGTCAGAATGAGACAAGAGATGTGCTTGTTTGG AGCAATGATCGGGTGATTCGCTGGATCCTGTCGATCGGCCTTAAAGAGTTTGCCAACAACCTAATAGAGA GCGGTGTTCACGGCGCACTTCTGGCCTTGGACGAGACGTTCGATTTCAGCGCCCTGGCACTCTTGTTACA GATTCCAACACAGAATACACAGGCTCGCGCTGTCTTGGAAAGAGAATTCAACAACCTTTTGGTCACCGGG ACTGATCGGAGGTTTGATGAGGATGATGACAAAAGCTTCAGGAGGGCACCTTCCTGGAGGAAGAAGTTCA GACCAAAGGACATCCGTGGCTTGGCTTCTGGCTCTGCAGAGACGTTGCCTGCCAACTTCCGGGTCACCTC CTCTATGTCATCTCCCTCTATGCAGCCAAAGAAGGTTCAGATGGACGTTTACCCACACTACTTCTACAGA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106320
Insert Size	3573 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106320.2, NP_001099790.2
RefSeq Size	4789 bp
RefSeq ORF	3573 bp
Locus ID	293645

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.