Tonsl (NM_001130572) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nfkbil2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210543 representing NM_001130572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCTGGAACAGGAGCTTCGCCAGCTGAGCAAGGCGAAGGCCAGGGCCCAGAGGAATGGACAGCTGT GCGAGGAGGCGGTCTGCTGCCACCAGCTGGGGGAGCTGCTAGCTAGCCACGGTCGCTTCCAGGAAGCTTT AGAGGAGCACCAGCAGGAGCTACATCTGCTAGAGAGTGTCCAGGACACCCTAGGCTGCGCTGTGGCCCAC CGCAAGATCGGAGAGCGGCTGGCTGAAATGGAGAACTACTCTGCTGCTCTGAAGCACCAGCATCTCTACC TGGATCTAGCTGGTTCCCTGTCCAACCACACTGAATTGCAGAGAGCCTGGGCCACCATTGGCCGCACCCA TCTGGATGTATATGACCACTGCCAATCAAGAGATTCCTTGCTGCAGGCACAGGCTGCCTTTGAGAAGAGT TTGGCTATTGTGGATGAGAAACTAGAGGGGATGCTGACCCAGCGGGAACTGAGTGAGATGAGGACCCGTC TCTACCTCAATCTGGGCCTCACCTGTGAGAGCCTGCAGCAGACAGCCCAGTGCAACAACTACTTCAAAAA GAGCATCTTTCTCGCTGAGCAGAACCATCTGTATGAAGATCTGTTCCGGGCCCGATACAACCTGGGTGCC ATCCACTGGCGCGGAGGGCAGCACTCTCAGGCCATGCGCTGCTTGGAGGGGGCCCGGGAATGTGCACGTG CCATGAAGATGAGATTCATGGAAAGCGAATGTTGCATGCTAGTGTCTCAGGTGCTCCAAGATCTGGGGGA CTTTCTGGCTGCCAAACGAGCCCTGAAGAAGGCCTATAGGTTGGGCTCTCAGAAGCCTAACCAGAGAGTG GCTATCTGTCAGAGTCTCAAGTATGTATTGGCCGTGGTCCGGCTGCAGCAGCAGCTGCAAGAGGCTGAGG GCAATGATCTTCAAGGTGCCATGGCTATCTGTGAACAGTTGGGGGACCTTTTCTCCAAAGCCGATGACTT CCCCAAGGCATCTGAGGCTTACCAGAAGCAGCTGCACTTTGCTGAGCTGCTGAACAGACCGGACCTCGAG CTGGCTGTCATCCACGAATCCCTGGCCACCACGCTGGGAGACATGAAGGATTACCACAAGGCTGTACACC ACTATGAAGAGGAACTGAGGCTACGCAAAGGCAATGCCCTGGAGGAAGCTAAGACTTGGTTCAACATTGG ACTGGCCCGTGAGGAAGCTGGAGATGCATATGAGCTGCTAGCTCCATGCTTCCAGAAGGCTTTCGGCTGT GCTCAGCAGGCCCAGCGGTACCAACTGCAGAGGCAGATCTTACAGCATCTTTACACTGTGCAACTGAAGT TGCAGCCCCAAGAAGCCCGTGACACTGAAATCAGACTGCAGGAGCTGAGTATGGCAAAAGACACAGAGGA AGAGGAGGAAGAAGAGGAGGAGGAAGAGGAAGAAGCCAGTGAGGCCCTGGAGACCAGTGAAATGGAGCTC TCAGAGAGCGAGGATGATGCTGACGGCCTGTCTCAGCAGCTGGAGGAAGAGGAGGAGCTTCAGGGCTGTG TGGGGCGGCGGAAGATAAACAAGTGGAACCGGCGCAATGACATGGGAGAGACCCTGTTGCACCGAGCTTG CATCGAAGGCCAACTGCGCCGTGTCCAGGATCTTGTGAAGCAGGGCCATCCCCTGAATCCCCGAGACTAC TGTGGCTGGACTCCTCTACATGAAGCTTGCAACTATGGACATCTTGAGATTGTGCGCTTCCTTCTGGACC ATGGAGCAGCAGTGGATGACCCGGGTGGCCAGGGGTGTGATGGCATCACCCCCCTGCATGATGCCCTCAA CTGTGGCCACTTTGAGGTAGCTGAGCTCCTCATTGAGCGAGGGGCGTCCGTAACTCTCCGCACCAGGAAG GGCCTCAGCCCACTGGAGACACTGCAGCAGTGGGTGAAGCTGTACTTCAGGGATCTTGACCTTGAGACAA GACAGAAGGCCGCATCCATGGAGAGGAGGCTCCAGATGGCCTCCTCAGGCCAAGCTTCCCACAGCTCCCC TGCCCTCCAGACCATTCCAAATAATCATCTCTTCGACCCTGAGACCTCTCCTCCCTCAAGCCCCTGTCCA AAACCCCCCTCGTATACTCCTAGACCTCCAGAGGCCTCTCCAGCCCCTGCTAAGGTCTTTCTGGAGGAAA CTGTGTCTGCAGTGTGCAGACCTCGAAAGAACAGGCACAGGCCAGCCAGCAGCAGTAGCAGCTCGGAGGA CGATGACGACAACACAAACCCCTGCAGGCCATCTCAGAAAAGACTGAGACACAGCACACAGCAGGGTGAA GCCAAGACACCTGACCCATCCAAAAGTAGAGAGACAGCCATTTCAAGCGCTTGCCGGGCTGCTTACCAAG CAGCCATCCGAGGCGTGGGGAGTGCCCAGAGCCGTCGCTTGGTACCTAGCCTGCCTCGGGGCTCAAATGA AGTCCCTGCCCCCAAAACAGCACTCATTCCTGAGGAGGAATTCCTGGCTGAGGAATGGCTGGAGGTAGAT ACACCTCTGACCCGCAGCAGCAGCAGCAGCAGGCCCAGTACCTCCATATCAGACTATGAGCGATGCCCTG CAAGGCCCCGGACTCGTGCCAAGCAGAGTCGCCCAGCAAGTCTTGATGGTTGGTGTACACGGACTAAAGC AGCAGATGCCAGCTTGACTGCAGAGCCTACGGAGAACTCCAGCATGCCAAGGACCACAGGACCCAACAAA GAAAATTGTGCAGCAGGCCAGCCTTTGCTTCTGGTCCAGCCCCCTCCTATCCGGGTTCGAGTTCAAATTC AGGACAACCTTTTCCTCATCCCCGTCCCACACAGCGATGTCCATTCTGTGGCTTGGCTAGCAGAGCAAGC GGCCCAGCGCTACTTCCAAACTTGCGGGCTACTCCCAAGGCTCACCCTACGGAAGGATGGAGCACTACTG GCCCCACAGGACCCCATCCCTGATGTGCTGCAAAGTAACGACGAGGTGATGGCTGAAGTAACGTCATGGG ACCTTCCTCCACTAAAGGACCGCTACCGTAGGGCCTGCCAGAGCCTGGGGCAAGGGGAGCACCAGCAAGT GCTGCAGGCCATGGAGCACCAGAGTTCGAGCCCTTCGTTTAGTGCCTGTTCTCTGGCCTTGTGCCAAGCC CAGCTCACACCCCTGCTGAGGGCCCTCAAGTTACACACAGCACTCCGGGAGCTACGCCTTTCTGGGAACC GACTGGGTGACCCATGTGCTACTGAGCTACTGGCTACCCTGGGCACCATGCCTAACCTGGTCCTCCTCGA TCTCTCTTCCAATCACTTGGGCCCGGAAGGCCTGCGTCAGCTTGTTGAAGGCTCCTTGGGCCAGACTGCT TTCCAGAACGTGGAGGAACTAGACTTGAGTATGAATCCACTGGGAGATGGCTGTGCTCAGGCCCTGGCCT CCCTTCTGCGGACCTGCCCCGTGCTCAGGACCTTGCGTCTACAAGCCTGTGGTTTCAGCCCCAGCTTCTT CCTGAGCCACCAGGCTGCCCTGGGTAGTGCCTTCAAAGATGCTGAGCACCTCAAGACCTTGTCCCTATCT TACAATACTCTCGGCGCTCCTGCCCTGGCAAGGGTGCTGCAGAGCTTACCCACCTGTACCCTCCTACATT TGGAGCTTAGCTCCGTGGCAGCCAGCAAGAGCAACTCGAGTCTCATAGAGCCTGTTATCAAATACTTGAC CAAGGAAGGCTGTGCTCTGGCCCACCTAACCCTGTCTGCAAACTGCCTGAGTGACAAGGCTGTGAGAGAA CTGAGCAGATGTCTCCCTAGCTGCCCCTCACTCACCTCTCTGGACCTGTCCGCCAACCCTGAGGTCAGCT GTGCCGGTCTGGAGGAGCTCCTGTCTGCCCTACAGGAGCGGCCCCAAGGCCTCAGCTTCTTTGACTTGTC AGGCTGCTCCATTCAGGGGCCCCTGAACTCTGACCTCTGGGACAAGATCCTCTCGCAGTTGCAGGAGCTG CAGTTGTGCAGCAAAGACCTGACCACAAAAGACCGAGACACCCTGTGTCAGAGACTGCCGGCGGGTGCGT GCACACTGAACCAAGGTTCCAAGCTCTTCTTTAAATGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001130572
Insert Size	4104 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130572.1, NP_001124044.1
RefSeq Size	4246 bp
RefSeq ORF	4104 bp
Locus ID	366953
UniProt ID	D4A615
Gene Summary	Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold (By similarity).[UniProtKB/Swiss-Prot Function]

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