Kidins220 (NM_053795) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ARMS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210662 representing NM_053795 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTTCTTATATCACAGAGTGTGATAAATTATGTGGAAGAAGAGAATATTCCTGCTCTGAAAGCTC TTCTTGAGAAGTGCAAAGATGTAGATGAGAGGAACGAGTGTGGCCAGACGCCGCTGATGCTGGCTGCGGA GCAGGGCAATGTGGAAATAGTGAAAGAACTGTTAAAGAATGGAGCCAACTGCAATCTGGAAGATCTGGAT AACTGGACTGCCCTTATATCGGCATCTAAGGAGGGGCACATCCACATTGTGGAGGAGCTGCTGAAGAGCG GGGCCAGTCTGGAGCACCGAGACATGGGTGGATGGACAGCTCTCATGTGGGCGTGCTACAAAGGCAGGAC CGACGTGGTCGAGCTGCTTCTTTCTCATGGTGCCAACCCAAGTGTCACTGGTTTGTATAGTGTCTACCCA ATCATCTGGGCAGCGGGCAGAGGCCATGCCGACATAGTGCATCTTCTACTGCAGAATGGTGCGAAAGTCA ATTGCTCCGACAAGTACGGAACAACCCCCTTGGTATGGGCTGCCCGAAAGGGTCATTTGGAATGTGTGAA GCACTTGTTGGCCATGGGAGCTGATGTTGATCAAGAAGGAGCCAATTCGATGACTGCACTTATCGTGGCA GTGAAAGGAGGCTACACACAGTCCGTGAAAGAGATTTTGAAAAGGAATCCAAATGTGAACCTAACTGACA AGGATGGAAACACAGCTCTGATGATCGCATCGAAGGAGGGACATATTGAGATTGTGCAGGACCTGCTGGA CGCCGGAACATACGTGAACATACCTGACAGGAGTGGGGATACTGTATTGATCGGCGCTGTGAGAGGTGGT CATGTTGAAATTGTTCGAGCACTTCTCCAAAAATATGCTGATATAGACATTAGAGGGCAGGACAATAAGA CCGCGCTGTATTGGGCCGTTGAAAAGGGAAATGCGACAATGGTGAGGGATATCCTGCAGTGCAATCCGGA CACGGAAATATGCACGAAGGATGGTGAGACACCACTGATAAAAGCCACCAAGATGAGAAATATTGAAGTG GTGGAGCTGCTGCTGGATAAGGGGGCTAAGGTGTCTGCTGTGGACAAGAAAGGGGACACTCCCCTGCACG TTGCCATCCGAGGGAGGAGCCGGAGACTGGCCGAACTTCTTTTACGGAACCCCAAAGATGGACGGTTACT CTACAGACCCAACAAAGCAGGCGAGACTCCCTACAACATTGACTGCAGCCACCAGAAAAGCATTTTAACT CAAATATTTGGAGCCAGACACTTGTCTCCTACGGAAACAGATGGCGACATGCTTGGTTATGATTTGTATA GCAGTGCCCTGGCCGATATTCTCAGTGAACCCACCATGCAACCACCCATTTGTGTGGGCTTGTATGCACA GTGGGGAAGTGGGAAGTCTTTCTTACTCAAGAAACTAGAAGATGAGATGAAGACGTTTGCAGGACAGCAG ACCGAGCCCCTCTTCCAGTTCTCGTGGCTCATAGTGTTCCTCACCCTGCTGCTGTGTGGCGGGCTCGGCC TAGTGTTTGCTTTCACGGTTGATACAAACCTCGCCATAGCCATTTCATTGAGTTTCCTGGCTCTCATATA TATATTCTTCATCGTCATTTACTTCGGTGGACGGCGGGAAGGAGAGAGTTGGAACTGGGCCTGGGCTCTC AGTACCAGACTGGCAAGACACATTGGGTACCTGGAGCTCCTCTTCAAACTGATGTTTGTGAACCCACCTG AACTGCCGGAGCAAACTACAAAGGCTTTACCTGTGAGGTTTTTGTTTACAGATTACAATAGACTCTCCAG TGTGGGAGGAGAAACTTCCTTGGCTGAAATGATCGCGACCCTCTCAGATGCTTGTGAGAGAGAATTCGGC TTTTTGGCTACCCGTCTTTTTCGAGTCTTCAGGACTGAAGAGTCTCAGGGTAAAAAGAAATGGAAAAAAA CATGCTGCCTCCCCTCTTTTGTCATCTTTCTTTTTATTGTTGGCTGCATTATTGCTGGGATCACCCTTCT GGCTATATTCAGAGTTGACCCGAAACATCTGACAGTGAATGCTATCCTCATTTCTATCGCATCTGTCGTG GGGTTGGCCTTCGTGCTCAATTGTCGAACATGGTGGCAAGTGCTGGACTCTCTCCTGAATTCTCAGAGAA AACGCCTCCATAGTGCTGCCTCCAAATTACACAAGTTAAAAAGTGAAGGATTCATGAAAGTCCTGAAGTG TGAGGTGGAGCTGATGGCCAGGATGGCAAAAACCATTGACAGCTTCACTCAAAACCAGACAAGGCTGGTG GTTATCATTGACGGCCTGGATGCCTGCGAGCAGGATAAAGTCCTTCAGATGCTGGACACTGTCCGAGTTC TGTTTTCAAAGGGCCCTTTTATTGCCATTTTTGCAAGTGATCCGCATATTATCATAAAGGCCATCAACCA GAACCTGAATAGTGTGCTTCGTGACTCAAATATAAACGGACATGACTACATGCGCAATATAGTTCACTTA CCAGTTTTTCTTAATAGTCGTGGGCTTAGCAATGCAAGGAAATTCCTTGTAACTTCAGCAACAAATGGGG ACATTACATGCTCAGACACCACAGGAACACAGGAGGACACTGACAGAAGAGTTTCACAGAACAGCCTTGG GGAGATGACAAAGCTTGGGAGCAAAACGGCTCTCAACAGAAGGGACACTTACCGCAGAAGACAGATGCAG AGAACCATCACGAGGCAGATGTCCTTTGATCTCACAAAGCTGCTGGTCACTGAGGATTGGTTCAGTGACA TCAGCCCTCAGACCATGAGACGGCTACTCAATATTGTTTCTGTGACAGGGCGGCTACTAAGAGCCAATCA GATTACTTTCAACTGGGACAGGCTAGCCAGCTGGATCAACCTCACCGAGCAGTGGCCGTACCGGACGTCT TGGCTTATACTATATCTGGAAGAGACTGAAGGTCTCCCTGATCAGATGACCTTAAAGACCATCTATGAGA GAATATCGAAGAATATCCCAACAACTAAAGATGTTGAGCCGCTTCTTGAAATTGATGGAGATATAAGAAA CTTTGAAGTCTTCTTGTCTTCAAGGACTCCAGTTCTCGTGGCTCGAGATGTAAAGACCTTTTTGCCGTGC ACAGTAAACCTGGATCCCAAACTTCGGGAGATCATCGCAGATGTCCGAGCTGCAAGAGAGCAGATCAACA TAGGAGGCCTGGCCTATCCCCCGCTCCCTCTGCACGAAGGCCCTCCTCGGCCACCTTCTGGGTACAGTCA GCCTGCATCTGTCTGCTCTTCAGCATCTTTCAACGGGCCGTTCCCAGGCGGGGTTGTGTCTCCACAGCCC CACAGCAGTTACTACAGCGGCTTGTCCGGACCCCAGCACCCCTTCTACAACAGGCCATTCTTTGCCCCAT ACCTTTACACGCCAAGGTATTACCCTGGCGGTTCCCAACATCTCATCTCACGTTCATCAGTAAAAACGAG TTTGCCCAGAGATCAGAACAATGGCCTACCGTGTGACTCTGGGTTTAACAAACAGAGACAGGCAGCCGTC CCTGCCACAGGCAGCTCACTGTTACTGAGTTCAATGACCGTGGATGTCGTATGTGAGAAACTGAGACAGA TAGAAGGGCTGGACCAGAGCATGATGCCTCAGTACTGCACAACAATCAAAAAGGCGAACATAAATGGCCG GGTATTGTCTCAGTGTAACATTGATGAGCTGAAGAAAGAGATGGCTATGAACTTTGGAGACTGGCATCTC TTCAGAAGCATGGTCTTAGAAATGAGAAGTGTGGAAAGCCAGGTGGTCCCTGAAGACCCTCGTTTTCTCA ATGAAAACTCCAGTGCCCCAGTTCCTCATGGAGAGTCTGCTCGCCGCTCTTCTCACACTGAGCTGCCCCT CACGGAGCTTTCCAGCCAGACTCCATACACCCTGAACTTCAGCTTTGAAGAACTGAACACGCTTGGCCTT GATGAGGGAGCCCCTAGGCACAGTAACCTGAGTTGGCAGTCACAAACTCGCAGAACCCCAAGTCTCTCGA GTCTCAATTCCCAGGACTCCAGTATTGAAATTTCAAAGCTTACTGATAAGGTGCAGGCCGAGTATAGAGA CGCCTATAGAGAGTACATTGCTCAGATGTCCCAGTTAGAAGGGGGCACAGGGTCGTCCACAATAAGCGGC AGATCTTCTCCACATAGCACATACTACATAGGCCAGAGCTCTTCAGGGGGCTCCATCCATTCTACTCTAG AACAGGAAAGGGGGAAGGAGGGTGAGCTAAAGCAGGAGGATGGGCGGAAGTCATTTTTAATGAAGAGGGG GGATGTCATAGATTACTCCTCATCAGGGGTGTCCACTAATGAGGCCTCACCCCTGGACCCCATTACTGAA GAAGATGAGAAATCCGACCAGTCAGGTAGTAAGCTACTCCCAGGCAAGAAATCCTCAGAACGGCCCAGTC TCTTCCAGACAGACTTGAAGCTTAAGGGGGGTGGTCTTCGCTACCAGAAACTGCCCAGTGATGAAGATGA GTCGGGGACAGAAGAGTCAGATAACACCCCACTGCTCAAAGATGATAAGGACAAAAAGGCTGAAGGCAAA GCAGAGAGAGTGTGCAAGTCCCCAGAGCACAGTGCCGAGCCCATCAGAACGTTCATTAAAGCAAAAGAGT ATTTGTCCGATGCCCTCCTTGACAAAAAGGATTCCTCAGATTCGGGAGTGAGGTCCAATGAGAGTTCTCC CAACCACTCTCTCCACAATGAGGCAGCAGACGACTCCCAACTGGAAAAGGCAAACCTCATAGAGCTTGAA GATGAGGGCCACAGTGGGAAGCGGGGGATGCCACACAGTCTGAGTGGCTTGCAAGATCCAATTATAGCTC GGATGTCCATTTGCTCGGAAGACAAGAAAAGCCCTTCCGAGTGCAGCCTGATTGCTAGCAGCCCTGAAGA AAGCTGGCCTGCGTGCCAGAAGGCCTACAATCTAAACCGAACGCCCAGCACTGTGACTCTGAACAACAAC ACTGCACCCACCAACAGAGCCAATCAGAACTTCGATGAGATAGAGGGAATCAGGGAGACGTCCCAGGTCA TTCTGAGGCCTGGCCCCAGTCCCAACCCAACTGCTGTGCAGAATGAAAACCTGAAGAGCATGGCGCACAA GCGAAGCCAGCGCTCCAGTTACACAAGGCTCTCGAAAGATGCATCTGAGCTACATGCAGCCTCCTCGGAG AGCACAGGCTTTGGAGAAGAGAGAGAGAGCATTCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053795
Insert Size	5289 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053795.1, NP_446247.1
RefSeq Size	7140 bp
RefSeq ORF	5289 bp
Locus ID	116478
UniProt ID	Q9EQG6
Gene Summary	developing nervous system protein which is a substrate for protein kinase D and a downstream target for both neurotrophin and ephrin receptors [RGD, Feb 2006]

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