Kidins220 (NM_053795) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARMS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210662 representing NM_053795 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTTCTTATATCACAGAGTGTGATAAATTATGTGGAAGAAGAGAATATTCCTGCTCTGAAAGCTC TTCTTGAGAAGTGCAAAGATGTAGATGAGAGGAACGAGTGTGGCCAGACGCCGCTGATGCTGGCTGCGGA GCAGGGCAATGTGGAAATAGTGAAAGAACTGTTAAAGAATGGAGCCAACTGCAATCTGGAAGATCTGGAT AACTGGACTGCCCTTATATCGGCATCTAAGGAGGGGCACATCCACATTGTGGAGGAGCTGCTGAAGAGCG GGGCCAGTCTGGAGCACCGAGACATGGGTGGATGGACAGCTCTCATGTGGGCGTGCTACAAAGGCAGGAC CGACGTGGTCGAGCTGCTTCTTTCTCATGGTGCCAACCCAAGTGTCACTGGTTTGTATAGTGTCTACCCA ATCATCTGGGCAGCGGGCAGAGGCCATGCCGACATAGTGCATCTTCTACTGCAGAATGGTGCGAAAGTCA ATTGCTCCGACAAGTACGGAACAACCCCCTTGGTATGGGCTGCCCGAAAGGGTCATTTGGAATGTGTGAA GCACTTGTTGGCCATGGGAGCTGATGTTGATCAAGAAGGAGCCAATTCGATGACTGCACTTATCGTGGCA GTGAAAGGAGGCTACACACAGTCCGTGAAAGAGATTTTGAAAAGGAATCCAAATGTGAACCTAACTGACA AGGATGGAAACACAGCTCTGATGATCGCATCGAAGGAGGGACATATTGAGATTGTGCAGGACCTGCTGGA CGCCGGAACATACGTGAACATACCTGACAGGAGTGGGGATACTGTATTGATCGGCGCTGTGAGAGGTGGT CATGTTGAAATTGTTCGAGCACTTCTCCAAAAATATGCTGATATAGACATTAGAGGGCAGGACAATAAGA CCGCGCTGTATTGGGCCGTTGAAAAGGGAAATGCGACAATGGTGAGGGATATCCTGCAGTGCAATCCGGA CACGGAAATATGCACGAAGGATGGTGAGACACCACTGATAAAAGCCACCAAGATGAGAAATATTGAAGTG GTGGAGCTGCTGCTGGATAAGGGGGCTAAGGTGTCTGCTGTGGACAAGAAAGGGGACACTCCCCTGCACG TTGCCATCCGAGGGAGGAGCCGGAGACTGGCCGAACTTCTTTTACGGAACCCCAAAGATGGACGGTTACT CTACAGACCCAACAAAGCAGGCGAGACTCCCTACAACATTGACTGCAGCCACCAGAAAAGCATTTTAACT CAAATATTTGGAGCCAGACACTTGTCTCCTACGGAAACAGATGGCGACATGCTTGGTTATGATTTGTATA GCAGTGCCCTGGCCGATATTCTCAGTGAACCCACCATGCAACCACCCATTTGTGTGGGCTTGTATGCACA GTGGGGAAGTGGGAAGTCTTTCTTACTCAAGAAACTAGAAGATGAGATGAAGACGTTTGCAGGACAGCAG ACCGAGCCCCTCTTCCAGTTCTCGTGGCTCATAGTGTTCCTCACCCTGCTGCTGTGTGGCGGGCTCGGCC TAGTGTTTGCTTTCACGGTTGATACAAACCTCGCCATAGCCATTTCATTGAGTTTCCTGGCTCTCATATA TATATTCTTCATCGTCATTTACTTCGGTGGACGGCGGGAAGGAGAGAGTTGGAACTGGGCCTGGGCTCTC AGTACCAGACTGGCAAGACACATTGGGTACCTGGAGCTCCTCTTCAAACTGATGTTTGTGAACCCACCTG AACTGCCGGAGCAAACTACAAAGGCTTTACCTGTGAGGTTTTTGTTTACAGATTACAATAGACTCTCCAG TGTGGGAGGAGAAACTTCCTTGGCTGAAATGATCGCGACCCTCTCAGATGCTTGTGAGAGAGAATTCGGC TTTTTGGCTACCCGTCTTTTTCGAGTCTTCAGGACTGAAGAGTCTCAGGGTAAAAAGAAATGGAAAAAAA CATGCTGCCTCCCCTCTTTTGTCATCTTTCTTTTTATTGTTGGCTGCATTATTGCTGGGATCACCCTTCT GGCTATATTCAGAGTTGACCCGAAACATCTGACAGTGAATGCTATCCTCATTTCTATCGCATCTGTCGTG GGGTTGGCCTTCGTGCTCAATTGTCGAACATGGTGGCAAGTGCTGGACTCTCTCCTGAATTCTCAGAGAA AACGCCTCCATAGTGCTGCCTCCAAATTACACAAGTTAAAAAGTGAAGGATTCATGAAAGTCCTGAAGTG TGAGGTGGAGCTGATGGCCAGGATGGCAAAAACCATTGACAGCTTCACTCAAAACCAGACAAGGCTGGTG GTTATCATTGACGGCCTGGATGCCTGCGAGCAGGATAAAGTCCTTCAGATGCTGGACACTGTCCGAGTTC TGTTTTCAAAGGGCCCTTTTATTGCCATTTTTGCAAGTGATCCGCATATTATCATAAAGGCCATCAACCA GAACCTGAATAGTGTGCTTCGTGACTCAAATATAAACGGACATGACTACATGCGCAATATAGTTCACTTA CCAGTTTTTCTTAATAGTCGTGGGCTTAGCAATGCAAGGAAATTCCTTGTAACTTCAGCAACAAATGGGG ACATTACATGCTCAGACACCACAGGAACACAGGAGGACACTGACAGAAGAGTTTCACAGAACAGCCTTGG GGAGATGACAAAGCTTGGGAGCAAAACGGCTCTCAACAGAAGGGACACTTACCGCAGAAGACAGATGCAG AGAACCATCACGAGGCAGATGTCCTTTGATCTCACAAAGCTGCTGGTCACTGAGGATTGGTTCAGTGACA TCAGCCCTCAGACCATGAGACGGCTACTCAATATTGTTTCTGTGACAGGGCGGCTACTAAGAGCCAATCA GATTACTTTCAACTGGGACAGGCTAGCCAGCTGGATCAACCTCACCGAGCAGTGGCCGTACCGGACGTCT TGGCTTATACTATATCTGGAAGAGACTGAAGGTCTCCCTGATCAGATGACCTTAAAGACCATCTATGAGA GAATATCGAAGAATATCCCAACAACTAAAGATGTTGAGCCGCTTCTTGAAATTGATGGAGATATAAGAAA CTTTGAAGTCTTCTTGTCTTCAAGGACTCCAGTTCTCGTGGCTCGAGATGTAAAGACCTTTTTGCCGTGC ACAGTAAACCTGGATCCCAAACTTCGGGAGATCATCGCAGATGTCCGAGCTGCAAGAGAGCAGATCAACA TAGGAGGCCTGGCCTATCCCCCGCTCCCTCTGCACGAAGGCCCTCCTCGGCCACCTTCTGGGTACAGTCA GCCTGCATCTGTCTGCTCTTCAGCATCTTTCAACGGGCCGTTCCCAGGCGGGGTTGTGTCTCCACAGCCC CACAGCAGTTACTACAGCGGCTTGTCCGGACCCCAGCACCCCTTCTACAACAGGCCATTCTTTGCCCCAT ACCTTTACACGCCAAGGTATTACCCTGGCGGTTCCCAACATCTCATCTCACGTTCATCAGTAAAAACGAG TTTGCCCAGAGATCAGAACAATGGCCTACCGTGTGACTCTGGGTTTAACAAACAGAGACAGGCAGCCGTC CCTGCCACAGGCAGCTCACTGTTACTGAGTTCAATGACCGTGGATGTCGTATGTGAGAAACTGAGACAGA TAGAAGGGCTGGACCAGAGCATGATGCCTCAGTACTGCACAACAATCAAAAAGGCGAACATAAATGGCCG GGTATTGTCTCAGTGTAACATTGATGAGCTGAAGAAAGAGATGGCTATGAACTTTGGAGACTGGCATCTC TTCAGAAGCATGGTCTTAGAAATGAGAAGTGTGGAAAGCCAGGTGGTCCCTGAAGACCCTCGTTTTCTCA ATGAAAACTCCAGTGCCCCAGTTCCTCATGGAGAGTCTGCTCGCCGCTCTTCTCACACTGAGCTGCCCCT CACGGAGCTTTCCAGCCAGACTCCATACACCCTGAACTTCAGCTTTGAAGAACTGAACACGCTTGGCCTT GATGAGGGAGCCCCTAGGCACAGTAACCTGAGTTGGCAGTCACAAACTCGCAGAACCCCAAGTCTCTCGA GTCTCAATTCCCAGGACTCCAGTATTGAAATTTCAAAGCTTACTGATAAGGTGCAGGCCGAGTATAGAGA CGCCTATAGAGAGTACATTGCTCAGATGTCCCAGTTAGAAGGGGGCACAGGGTCGTCCACAATAAGCGGC AGATCTTCTCCACATAGCACATACTACATAGGCCAGAGCTCTTCAGGGGGCTCCATCCATTCTACTCTAG AACAGGAAAGGGGGAAGGAGGGTGAGCTAAAGCAGGAGGATGGGCGGAAGTCATTTTTAATGAAGAGGGG GGATGTCATAGATTACTCCTCATCAGGGGTGTCCACTAATGAGGCCTCACCCCTGGACCCCATTACTGAA GAAGATGAGAAATCCGACCAGTCAGGTAGTAAGCTACTCCCAGGCAAGAAATCCTCAGAACGGCCCAGTC TCTTCCAGACAGACTTGAAGCTTAAGGGGGGTGGTCTTCGCTACCAGAAACTGCCCAGTGATGAAGATGA GTCGGGGACAGAAGAGTCAGATAACACCCCACTGCTCAAAGATGATAAGGACAAAAAGGCTGAAGGCAAA GCAGAGAGAGTGTGCAAGTCCCCAGAGCACAGTGCCGAGCCCATCAGAACGTTCATTAAAGCAAAAGAGT ATTTGTCCGATGCCCTCCTTGACAAAAAGGATTCCTCAGATTCGGGAGTGAGGTCCAATGAGAGTTCTCC CAACCACTCTCTCCACAATGAGGCAGCAGACGACTCCCAACTGGAAAAGGCAAACCTCATAGAGCTTGAA GATGAGGGCCACAGTGGGAAGCGGGGGATGCCACACAGTCTGAGTGGCTTGCAAGATCCAATTATAGCTC GGATGTCCATTTGCTCGGAAGACAAGAAAAGCCCTTCCGAGTGCAGCCTGATTGCTAGCAGCCCTGAAGA AAGCTGGCCTGCGTGCCAGAAGGCCTACAATCTAAACCGAACGCCCAGCACTGTGACTCTGAACAACAAC ACTGCACCCACCAACAGAGCCAATCAGAACTTCGATGAGATAGAGGGAATCAGGGAGACGTCCCAGGTCA TTCTGAGGCCTGGCCCCAGTCCCAACCCAACTGCTGTGCAGAATGAAAACCTGAAGAGCATGGCGCACAA GCGAAGCCAGCGCTCCAGTTACACAAGGCTCTCGAAAGATGCATCTGAGCTACATGCAGCCTCCTCGGAG AGCACAGGCTTTGGAGAAGAGAGAGAGAGCATTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210662 representing NM_053795 Red=Cloning site Green=Tags(s) MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMLAAEQGNVEIVKELLKNGANCNLEDLD NWTALISASKEGHIHIVEELLKSGASLEHRDMGGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYP IIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVA VKGGYTQSVKEILKRNPNVNLTDKDGNTALMIASKEGHIEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGG HVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEV VELLLDKGAKVSAVDKKGDTPLHVAIRGRSRRLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILT QIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQ TEPLFQFSWLIVFLTLLLCGGLGLVFAFTVDTNLAIAISLSFLALIYIFFIVIYFGGRREGESWNWAWAL STRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFG FLATRLFRVFRTEESQGKKKWKKTCCLPSFVIFLFIVGCIIAGITLLAIFRVDPKHLTVNAILISIASVV GLAFVLNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLV VIIDGLDACEQDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHL PVFLNSRGLSNARKFLVTSATNGDITCSDTTGTQEDTDRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQ RTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTS WLILYLEETEGLPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEVFLSSRTPVLVARDVKTFLPC TVNLDPKLREIIADVRAAREQINIGGLAYPPLPLHEGPPRPPSGYSQPASVCSSASFNGPFPGGVVSPQP HSSYYSGLSGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRSSVKTSLPRDQNNGLPCDSGFNKQRQAAV PATGSSLLLSSMTVDVVCEKLRQIEGLDQSMMPQYCTTIKKANINGRVLSQCNIDELKKEMAMNFGDWHL FRSMVLEMRSVESQVVPEDPRFLNENSSAPVPHGESARRSSHTELPLTELSSQTPYTLNFSFEELNTLGL DEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDAYREYIAQMSQLEGGTGSSTISG RSSPHSTYYIGQSSSGGSIHSTLEQERGKEGELKQEDGRKSFLMKRGDVIDYSSSGVSTNEASPLDPITE EDEKSDQSGSKLLPGKKSSERPSLFQTDLKLKGGGLRYQKLPSDEDESGTEESDNTPLLKDDKDKKAEGK AERVCKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSNESSPNHSLHNEAADDSQLEKANLIELE DEGHSGKRGMPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEESWPACQKAYNLNRTPSTVTLNNN TAPTNRANQNFDEIEGIRETSQVILRPGPSPNPTAVQNENLKSMAHKRSQRSSYTRLSKDASELHAASSE STGFGEERESIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053795
ORF Size	5286 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053795.1, NP_446247.1
RefSeq Size	7140 bp
RefSeq ORF	5289 bp
Locus ID	116478
UniProt ID	Q9EQG6
MW	195.7 kDa
Gene Summary	developing nervous system protein which is a substrate for protein kinase D and a downstream target for both neurotrophin and ephrin receptors [RGD, Feb 2006]

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