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Tert (NM_053423) Rat Untagged Clone
CAT＃: RN210889
Tert (untagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug)
CNY 18,150.00
货期*
7周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN210889 representing NM_053423
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGCTCCTCGTTGCCCCGCCGTGCGCTCTCTACTGCGCAGCCGATATCGGGAGGTGTGGCCGC TGGCGACCTTTGTGCGGCGCCTGGGGCTTGAGGGCAGTCGGCTTGTGCAACCCGGGGACCCGAAGGTCTT CCGCACGTTGGTTGCCCAGTGCCTAGTGTGCGTGCCCTGGGGCTCACAGCCGCCACCTGCTGACCTTTCC TTCCACCAGGTGTCATCCCTGAAAGAGCTGGTGTCCAGGGTTGTGCAGAAACTTTGCGAGCGCGGTGAGA GGAATGTGCTGGCTTTTGGCTTTGCACTGCTTAACGGGGCCAGAGGTGGGCCTCCCATGGCCTTCACGAC CAGCGTGCATAGCTACTTGCCCAACTCGGTTACTGAGTCCCTGTGTGTCAGTGGTGCATGGATGCTACTG TTGAGCCGAGTGGGCGACGACCTGCTGGTCTACCTGCTGTCGCACTGTGCGCTCTACCTGCTGGTGCCCC CCAGCTGTGCCTACCAGGTGTGCGGGTCACCCCTGTACCAAATTTGTGCCACCACGGATACCTGGTCCTC TGTGCCCGCTGGTTACAGGCCCACTCGACCCGTGGGCGGGAATTTCACTAACCTTGGGTCCGCACACCAG ATCAAAAACAGTGGTCACCAGGAAGCACCAAAACCCCAGGCCTTGCCATCACGAGGTACGAAGAGGCTTC TGAGTCTCACCAGTACAAACGTGCCTTCAGCTAAGAAGGCCAGGTTTGAACCTGCCCTGAGAGTGGATAA GGGACCCCACAGGCAGGTGGTACCAACCCCATCAGGCAAAACATGGGCGCCAAGTCCTGCTGCGTCCCCC AAGGTGCCTCCTGCAGCGAAAAACTTGTCTTTGAAAGGAAAGGCATCTGACCCGAGTCTCTCTGGGTCGG TGTGCTGTAAACACAAGCCCAGCTCCTCGTCCCTGCTGTCATCACCACCCCAAGATGCTGAAAAGCTCAG GCCATTCACTGAGACCAGACATTTCCTTTACTCCAGGGGAGGTGGCCAAGAGGAGCTAAATCCCTCATTC CTACTCAACAGCCTCCCGCCTAGCTTGACCGGGGCCAGGAGACTGGTGGAGATCATCTTTCTGGGCTCAA GGCCTAGGACATCAGGACCATTCTGCAGGACCCGCCGCCTGCCCCGTCGATACTGGCAGATGCGACCCCT ATTCCAGCAGCTGCTCATGAACCACGCAAAGTGCCAATATGTCAGATTCCTCCGGTCGCACTGCAGATTT CGAACAGCAAACCAGCGGGTGCCGGATGCCATGGACACCAGCCCATCCCACCTCACGAGTTTGCTCCGGT TACACAGCAGCCCCTGGCAGGTATACGGCTTTCTTCGGGCCTGCCTCCGCGAGCTGGTGCCTGCCGGTCT CTGGGGCACCAGGCACAATGAGCGCCGCTTCTTAAAGAACGTGAAGAAGTTCATCTCGTTGGGGAAGTAC GCCAAGCTATCCCTGCAGGAACTGATGTGGAGGGTGAAAGTGGAGGACTGCCACTGGCTCCGCAGCAGCC CAGAGAAGGACACTGTCCCTGCCGCAGAGCACCGTCTGAGGGAGAGGATCCTTGCCATGTTCCTGTTCTG GCTAATGGACACATATGTGGTACAGCTGCTGAGGTCATTCTTCTACATCACAGAGACCACGTTCCAGAAG AACCGCCTTTTCTTCTACCGTAAGAGTGTGTGGAGCAAGCTGCAGAGCATTGGAATCAGGCAACAGCTTG AGAGAGTTCAGCTACGGGAACTGTCACAAGAGGAGGTCAAGCATCACCAGGACACTTGGCTGGCCATGCC TATCTGCAGATTGCGCTTCATCCCCAAGCTCAATGGTCTCCGGCCCATTGTGAACATGAGTTATGGCATG GACACCAGAGCTTTTGGCAAAAAGAAGCAGACCCAGTGTTTCACTCAGAGTCTCAAGACTTTGTTCAGCG TGCTCAACTACGAGCGGACCAAACATCCTAACCTTATGGGTGCTTCAGTACTGGGTACGAGTGACAGCTA CAGGATCTGGCGGACCTTCGTGCTGCGTGTGCGTGCTCTGGACCAGACACCCAGGATGTACTTTGTTAAG GCAGATGTGACAGGGGCCTATGATGCCATCCCCCAGGACAAGCTCGTGGAAATTGTCGCCAATATAATCA GGCGCTCAGAGAGCATGTACTGTATCCGCCAGTATGCAGTGGTTCAGAAAGATAGCCAAGGCCAAGTCCA CAAGTCCTTCAGGAGACAGGTCTCCACCCTCTCTGACCTCCAGCCATACATGGGCCAGTTCACCAAGCAT CTGCAGGACTCAGATGCCAGTGCACTGAGGAACTCTGTTGTCATCGAGCAGAGCATCTCCATGAATGAGA CTGGCAGTAGCCTGCTCCACTTCTTCCTGCGCTTTGTCCGTCACAGTGTCGTGAAGATCGATGGCAGGTT CTATGTGCAATGCCAGGGCATCCCCCAGGGCTCCAGCCTGTCCACCCTGCTCTGCAGTCTGTGTTTCGGA GACATGGAGAACAAGCTGTTTGCCGAGGTGCAGCAGGACGGCTTGCTTTTACGTTTTGTCGATGACTTTC TGTTGGTGACACCTCACCTGGCCCATGCAAAAGCCTTTCTCAGCACCCTGGTCCATGGCGTGCCCGAGTA TGGCTGCATGATAAACTTGCAGAAGACAGTGGTGAACTTCCCTGTGGAGACCGGCGCCCTGGGAGGTGCA GCCCCGCACCAGCTGCCTGCTCACTGCCTGTTTCCCTGGTGTGGCTTACTGCTGGACACTCGGACTTTGG AAGTATTCTGTGACTACTCAGGTTACGGACGGACCTCAATTAAGATGAGCCTCACCTTCCAGGGTGTCTC CAGGGCCGGGAAGACCATGCGGTACAAGCTCTTGTCAGTCTTGCGGTTGAAGTGTCATGGTCTGTTTCTA GACTTGCAGGTGAACAGCCTGCAGACAGTCTGCATCAATATATACAAGATCTTCCTGCTTCAGGCCTACA GGTTCCATGCATGTGTGATTCGGCTTCCCTTTGGCCAGCATGTTAGGAAGAACCATGCATTCTTTCTGGG CATCATCTCCAACCTAGCATCCTGCTGCTACGCCATCCTGAAGGTCAAGAATCCAGGAGTGTCACTAAGG GCCAAGGGTGCCCCTGGCTCCTTTCCGCCCGAGGCCACACGTTGGCTCTGCTACCAAGCCTTCCTGCTCA AGCTGGCTGCTCATTCTGTCACCTACAAGTGTCTCCTGGGACCTCTTAGGACAGCCCAAAAACAGCTGTG CCGGAAGCTCCCAGAGGCAACAATGACCCTCCTTAAGACTGCAGCTGACCCAGCCCTAAGCACAGATTTT CAGACCATTTTGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053423 |
| Insert Size | 3378 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053423.1, NP_445875.1 |
| RefSeq Size | 3378 bp |
| RefSeq ORF | 3378 bp |
| Locus ID | 301965 |
| UniProt ID | Q673L6 |
| Gene Summary | Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR210889 | Tert (Myc-DDK-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 18,150.00 |
|
| RR210889L3 | Lenti ORF clone of Tert (Myc-DDK-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 19,570.00 |
|
| RR210889L4 | Lenti ORF clone of Tert (mGFP-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug) |
CNY 21,570.00 |
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