Eif3a (NM_001047087) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Eif3s10; ZH12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210968 representing NM_001047087 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCTATTTCCAGAGGCCGGAAAATGCCCTCAAGCGCGCCAACGAATTTCTTGAGGTTGGCAAAA AGCAGCCTGCCCTGGATGTTCTTTATGATGTTATGAAAAGTAAGAAACACAGAACATGGCAGAAGATACA CGAGCCAATTATGCTGAAATACTTGGAACTATGTGTGGATCTTCGTAAGAGCCACTTGGCTAAGGAAGGA TTATATCAATATAAGAACATTTGTCAGCAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATT TGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGACATAGAAGA TCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGACACTCAGGATCGT ACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTC TTCGAAACAATTCTAGAGTAGAGCGTCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCA GTATACTCGCAAGGCTGAGTTCCGCAAGCTGTGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGC CACCATAACCAAAGTACAGCAATCAATCTTAATAATCCAGAGAGCCAGTCCATGCATTTGGAAACCAGAC TTGTTCAGCTGGACAGTGCTATCAGCATGGAATTGTGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCA TGGACTATTTTCCTTGTCTAAAAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCA ACGGTGTTTTGGAAATCTGGAAATGCTTTGTTTCATGCATCTACACTTCATCGTCTTTATCATCTGTCTA GAGAAATGAGAAAGAATCTTACACAAGATGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCT TTCCATTCCTATTACTCCTGAGCGAACTGATATTGCACGACTTTTGGATATGGATGGTATTATAGTTGAG AAGCAGCGGCGCCTTGCCACATTGCTAGGTCTTCAAGCACCACCCACACGAATTGGACTTATTAATGATA TGGTCAGGTTTAATGTGCTACAATATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGA ATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAA GAACCAGAATTGCAACAATATGTGCCACAACTACAGAACAATACCATACTCCGTCTTCTGCAACAGGTGG CACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACCTCTCTGGTTCCTTTTGTTGATGCTTTCCAACT GGAACGGGCCATAGTAGATGCAGCTAGGCACTGTGACCTGCAGGTTCGTATTGACCATACTTCTCGGACT CTGAGTTTTGGATCAGACTTAAATTATGCAACTCGAGAAGATGCCCCAATTGGTCCTCATCTGCAGAGCA TGCCTTCAGAACAAATAAGAAATCAACTGACAGCCATGTCCTCGGTACTTGCAAAAGCGCTTGAAGTCAT CAAGCCTGCTCATATTCTGCAAGAGAAAGAAGAACAGCATCAGTTGGCAGTTACTGCGTATATTAAAAAT TCACGGAAAGAGCATCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAGAGAAAAGAGCGTCTTGAGA GTCTGAATATTCAACGTGAGAAGGAAGAACTAGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGGAAGGC TGAAGAAGAAAGGCTGCGTCAAGAGGCAAAGGAGCGAGAGAAGGAACGGATCCTTCAAGAACATGAACAA ATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAACAGATCAAGAAGACAGAGCTGGGTGCCAAAGCATTTA AAGATATTGACATTGAAGACCTTGAAGAATTGGATCCAGATTTTATTATGGCCAAACAAGTTGAACAACT AGAAAAAGAAAAGAAGGAACTTCAGGAACGCCTTAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGA GCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTACGAGGAACAAAGGGTTAAAGACATGGACC TGTGGGAACAACAAGAAGAAGAAAGAATTACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAACATAA GAATCGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGGCTCAAAGCTGCCCGGCAGTCT GTCTATGAGGAGAAACTGAAACAGTTTGAAGAGCGTTTAGCAGAAGAAAGGCATAATCGCCTGGAAGAAC GGAAGAGGCAACGGAAAGAAGAACGCAAAATAACTTACTACAGAGAAAAGGAAGAGGAAGAGCAGAGAAG GGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGAGCTGAGAGAGCAAAACGTGAGGAAGAA CTTAGAGAATATCAGGAGCGAGTCAAGAAACTGGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGG AAATTGAAGAAAGGGAAAGACGCAGAGAGGAAGAAAGGAGACTTGGTGAGGATCCACTTTCTAGGAAGGA CTCTCGTTGGGGAGATAGAGATTCAGAAGGCACCTGGAGAAAAGGACCAGAAGCTGACTCTGAGTGGAGA AGAGGCCCACCAGAAAAGGAGTGGAGACGAGAAACTCGGGATGATGAGAGGCCTCACAGGAGAGATGAGG ATCGGCTGAGGCGCTTGGGGGGGGACGATGAAGAGAGAGAGTCGTCTCTTAGACCAGATGATGATCGCAT CCCCAGGCGTGGCCTGGATGATGACAGAGGGCCTAGACGTGGTCCCGATGAAGATAGATTTTCTCGTAGA GGAACAGATGATGACCGTCCTTCCTGGCGTAATGCAGATGATGACCGGCCTCCCAGGCGAATTGGTGATG ATGACAGGGGAAGCTGGCGTCACACAGATGATGACAGACCACCCAGACGGGGACTGGATGATGACAGACC ACCCAGACGGGGACTGGATGATGAGAGAGGAAGCTGGCGCACAGCAGAAGAGGACAGAGGACCTAGACGT GGGATGGATGATGACCGGGGGCCAAGACGAGGAGGTGCTGATGATGAGCGGTCATCCTGGCGGAATGCTG ATGACGATAGGGGTCCCAGGAGGGGGATGGATGACGATAGGGGTCCCAGGCGAGGGTTGGATGATGATCG AGGACCTTGGAGGAATGCTGCTGAAGATAGGATTTCCAGGAGAGGTGCAGATGATGACCGGGGGCCTTGG AGAAATATGGATGACGATCGGGTTCCTAGACGGGGTGATGATGCAAGACCTGGTCCCTGGAGACCATTTG TCAAACCAGGTGGATGGAGAGAGAAAGAAAAGGCTAGAGAAGAGAGTTGGGGTCCACCTCGAGAATCAAG ACCACCAGAAGAACGTGAATGGGATAGAGACAAAGAGAAGGACAGAGATAATCAAGATCGAGAGGAGAAC GACAAGGACCTTGAACGTGATAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGTTTCAGAAGACCCA GGGATGAAGGTGGCTGGAGAAGAGGACCAGCAGAAGAATCTTCAAGCTGGAGAGATTCAAGTCGCCGTGA TGATAGGGACAGGGATGACCGACGGCGGGACAGAGATGATCGTCGTGATCTGAGAGACCTGAGAGACAGA AGGGATTTAAGAGATGATAGAGATCGGAGAGGCCCTCCCCTTAGATCAGAGCGAGAAGAAGCAAGCTCCT GGAGACGCACTGATGACAGGAAAGATGACCGGACTGAAGAAAGGGATCCACCTCGTCGTGTTCCTCCCCC AACTCTTTCAAGAGAGAGAGAAAGAGAGAGAGATCGAGAAGGTGAGAAAGAGAAAGCATCTTGGAGAGCT GAGAAAGATAGGGAGTCCCTTCGTCGTACTAAGAATGAAACTGATGAAGATGGATGGACCACAGTACGAC GTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001047087
Insert Size	4065 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001047087.2, NP_001040552.2
RefSeq Size	5139 bp
RefSeq ORF	4065 bp
Locus ID	292148
UniProt ID	Q1JU68
Gene Summary	RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.