Eif3a (NM_001047087) Rat Untagged Clone

CAT#: RN210968

Eif3a (untagged ORF) - Rat eukaryotic translation initiation factor 3, subunit 10 (theta) (Eif3s10), (10 ug)



  "NM_001047087" in other vectors (1)

CNY 19,950.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Eif3s10; ZH12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210968 representing NM_001047087
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCTATTTCCAGAGGCCGGAAAATGCCCTCAAGCGCGCCAACGAATTTCTTGAGGTTGGCAAAA
AGCAGCCTGCCCTGGATGTTCTTTATGATGTTATGAAAAGTAAGAAACACAGAACATGGCAGAAGATACA
CGAGCCAATTATGCTGAAATACTTGGAACTATGTGTGGATCTTCGTAAGAGCCACTTGGCTAAGGAAGGA
TTATATCAATATAAGAACATTTGTCAGCAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATT
TGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGACATAGAAGA
TCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGACACTCAGGATCGT
ACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTC
TTCGAAACAATTCTAGAGTAGAGCGTCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCA
GTATACTCGCAAGGCTGAGTTCCGCAAGCTGTGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGC
CACCATAACCAAAGTACAGCAATCAATCTTAATAATCCAGAGAGCCAGTCCATGCATTTGGAAACCAGAC
TTGTTCAGCTGGACAGTGCTATCAGCATGGAATTGTGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCA
TGGACTATTTTCCTTGTCTAAAAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCA
ACGGTGTTTTGGAAATCTGGAAATGCTTTGTTTCATGCATCTACACTTCATCGTCTTTATCATCTGTCTA
GAGAAATGAGAAAGAATCTTACACAAGATGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCT
TTCCATTCCTATTACTCCTGAGCGAACTGATATTGCACGACTTTTGGATATGGATGGTATTATAGTTGAG
AAGCAGCGGCGCCTTGCCACATTGCTAGGTCTTCAAGCACCACCCACACGAATTGGACTTATTAATGATA
TGGTCAGGTTTAATGTGCTACAATATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGA
ATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAA
GAACCAGAATTGCAACAATATGTGCCACAACTACAGAACAATACCATACTCCGTCTTCTGCAACAGGTGG
CACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACCTCTCTGGTTCCTTTTGTTGATGCTTTCCAACT
GGAACGGGCCATAGTAGATGCAGCTAGGCACTGTGACCTGCAGGTTCGTATTGACCATACTTCTCGGACT
CTGAGTTTTGGATCAGACTTAAATTATGCAACTCGAGAAGATGCCCCAATTGGTCCTCATCTGCAGAGCA
TGCCTTCAGAACAAATAAGAAATCAACTGACAGCCATGTCCTCGGTACTTGCAAAAGCGCTTGAAGTCAT
CAAGCCTGCTCATATTCTGCAAGAGAAAGAAGAACAGCATCAGTTGGCAGTTACTGCGTATATTAAAAAT
TCACGGAAAGAGCATCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAGAGAAAAGAGCGTCTTGAGA
GTCTGAATATTCAACGTGAGAAGGAAGAACTAGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGGAAGGC
TGAAGAAGAAAGGCTGCGTCAAGAGGCAAAGGAGCGAGAGAAGGAACGGATCCTTCAAGAACATGAACAA
ATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAACAGATCAAGAAGACAGAGCTGGGTGCCAAAGCATTTA
AAGATATTGACATTGAAGACCTTGAAGAATTGGATCCAGATTTTATTATGGCCAAACAAGTTGAACAACT
AGAAAAAGAAAAGAAGGAACTTCAGGAACGCCTTAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGA
GCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTACGAGGAACAAAGGGTTAAAGACATGGACC
TGTGGGAACAACAAGAAGAAGAAAGAATTACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAACATAA
GAATCGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGGCTCAAAGCTGCCCGGCAGTCT
GTCTATGAGGAGAAACTGAAACAGTTTGAAGAGCGTTTAGCAGAAGAAAGGCATAATCGCCTGGAAGAAC
GGAAGAGGCAACGGAAAGAAGAACGCAAAATAACTTACTACAGAGAAAAGGAAGAGGAAGAGCAGAGAAG
GGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGAGCTGAGAGAGCAAAACGTGAGGAAGAA
CTTAGAGAATATCAGGAGCGAGTCAAGAAACTGGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGG
AAATTGAAGAAAGGGAAAGACGCAGAGAGGAAGAAAGGAGACTTGGTGAGGATCCACTTTCTAGGAAGGA
CTCTCGTTGGGGAGATAGAGATTCAGAAGGCACCTGGAGAAAAGGACCAGAAGCTGACTCTGAGTGGAGA
AGAGGCCCACCAGAAAAGGAGTGGAGACGAGAAACTCGGGATGATGAGAGGCCTCACAGGAGAGATGAGG
ATCGGCTGAGGCGCTTGGGGGGGGACGATGAAGAGAGAGAGTCGTCTCTTAGACCAGATGATGATCGCAT
CCCCAGGCGTGGCCTGGATGATGACAGAGGGCCTAGACGTGGTCCCGATGAAGATAGATTTTCTCGTAGA
GGAACAGATGATGACCGTCCTTCCTGGCGTAATGCAGATGATGACCGGCCTCCCAGGCGAATTGGTGATG
ATGACAGGGGAAGCTGGCGTCACACAGATGATGACAGACCACCCAGACGGGGACTGGATGATGACAGACC
ACCCAGACGGGGACTGGATGATGAGAGAGGAAGCTGGCGCACAGCAGAAGAGGACAGAGGACCTAGACGT
GGGATGGATGATGACCGGGGGCCAAGACGAGGAGGTGCTGATGATGAGCGGTCATCCTGGCGGAATGCTG
ATGACGATAGGGGTCCCAGGAGGGGGATGGATGACGATAGGGGTCCCAGGCGAGGGTTGGATGATGATCG
AGGACCTTGGAGGAATGCTGCTGAAGATAGGATTTCCAGGAGAGGTGCAGATGATGACCGGGGGCCTTGG
AGAAATATGGATGACGATCGGGTTCCTAGACGGGGTGATGATGCAAGACCTGGTCCCTGGAGACCATTTG
TCAAACCAGGTGGATGGAGAGAGAAAGAAAAGGCTAGAGAAGAGAGTTGGGGTCCACCTCGAGAATCAAG
ACCACCAGAAGAACGTGAATGGGATAGAGACAAAGAGAAGGACAGAGATAATCAAGATCGAGAGGAGAAC
GACAAGGACCTTGAACGTGATAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGTTTCAGAAGACCCA
GGGATGAAGGTGGCTGGAGAAGAGGACCAGCAGAAGAATCTTCAAGCTGGAGAGATTCAAGTCGCCGTGA
TGATAGGGACAGGGATGACCGACGGCGGGACAGAGATGATCGTCGTGATCTGAGAGACCTGAGAGACAGA
AGGGATTTAAGAGATGATAGAGATCGGAGAGGCCCTCCCCTTAGATCAGAGCGAGAAGAAGCAAGCTCCT
GGAGACGCACTGATGACAGGAAAGATGACCGGACTGAAGAAAGGGATCCACCTCGTCGTGTTCCTCCCCC
AACTCTTTCAAGAGAGAGAGAAAGAGAGAGAGATCGAGAAGGTGAGAAAGAGAAAGCATCTTGGAGAGCT
GAGAAAGATAGGGAGTCCCTTCGTCGTACTAAGAATGAAACTGATGAAGATGGATGGACCACAGTACGAC
GTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001047087
Insert Size 4065 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001047087.2, NP_001040552.2
RefSeq Size 5139 bp
RefSeq ORF 4065 bp
Locus ID 292148
UniProt ID Q1JU68
Gene Summary RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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