Trpm8 (NM_134371) Rat Untagged Clone

CAT#: RN211342

Trpm8 (untagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug)



  "NM_134371" in other vectors (3)

CNY 11,544.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms CMR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211342 representing NM_134371
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTTCGAGGGAGCCAGGCTCAGCATGAGGAGCCGCAGAAATGGAACTCTGGGCAGCACCCGGACCC
TGTACTCCAGCGTGTCTCGGAGCACAGACGTGTCCTACAGTGAAAGTGATTTGGTGAATTTTATTCAGGC
AAATTTTAAAAAACGAGAATGCGTCTTCTTTACCAGAGACTCCAAGGCCATGGAGAGCATATGCAAGTGT
GGTTATGCCCAGAGCCAGCATATCGAAGGCACCCAGATCAACCAAAATGAGAAGTGGAACTACAAAAAAC
ACACCAAGGAGTTTCCAACAGACGCCTTTGGGGACATTCAGTTTGAGACTCTGGGGAAGAAAGGCAAGTA
CTTACGCTTATCCTGTGACACGGACTCTGAAACCCTCTACGAACTGCTGACCCAGCACTGGCACCTCAAA
ACACCCAACCTGGTCATCTCAGTGACGGGTGGAGCCAAAAACTTTGCTTTGAAGCCACGCATGCGCAAAA
TCTTCAGTCGGCTGATCTACATCGCTCAGTCTAAAGGGGCATGGATTCTTACCGGAGGCACTCATTACGG
TCTGATGAAGTACATAGGTGAAGTGGTGAGGGATAACACCATCAGCAGGAACTCGGAAGAGAACATCGTG
GCCATTGGCATAGCGGCCTGGGGCATGGTCTCCAACAGGGACACCCTCATCAGGAATTGTGATGATGAGG
GACATTTTTCAGCTCAATATATCATGGATGACTTCATGAGAGATCCTCTCTACATCCTGGACAACAATCA
TACCCACCTGCTGCTTGTGGACAACGGTTGTCATGGACACCCCACGGTGGAAGCCAAACTTCGGAATCAG
CTGGAGAAGTACATCTCTGAGCGCACCAGTCAAGATTCCAACTATGGTGGTAAGATCCCCATCGTGTGTT
TTGCCCAGGGAGGTGGAAGAGAAACTTTGAAAGCCATCAACACCTCTGTCAAAAGTAAGATCCCCTGTGT
GGTGGTGGAAGGCTCGGGGCAGATTGCCGATGTGATTGCCAGCCTGGTGGAGGTAGAGGATGTTTTAACC
TCTTCCATGGTCAAAGAGAAGCTGGTACGGTTTTTACCCCGCACTGTGTCCCGGCTGCCTGAAGAGGAGA
TTGAGAGCTGGATCAAATGGCTCAAAGAAATTCTTGAGAGCCCCCACCTCCTCACGGTCATCAAGATGGA
GGAGGCTGGAGACGAGGTCGTGAGCAGCGCCATTTCCTACGCGCTGTACAAAGCCTTCAGCACTAATGAA
CAAGACAAGGACAACTGGAACGGACAGCTGAAGCTTCTGCTGGAGTGGAACCAACTGGACCTTGCCAGTG
ATGAGATCTTCACCAATGACCGCCGCTGGGAGTCTGCCGACCTTCAGGAAGTCATGTTCACGGCCCTCAT
AAAGGACAGGCCCAAGTTTGTCCGCCTCTTCCTGGAGAATGGCCTCAACCTGCAGAAGTTCCTCACCAAT
GAAGTCCTCACGGAGCTCTTCTCCACCCACTTCAGCACCCTAGTGTACCGGAACCTGCAGATCGCCAAGA
ACTCCTACAACGATGCACTCCTTACCTTTGTCTGGAAGTTGGTGGCAAACTTCCGTAGAAGCTTCTGGAA
AGAGGACAGAAGCAGCAGGGAGGACTTGGATGTGGAACTCCATGATGCATCTCTCACCACCCGGCACCCC
CTGCAGGCTCTTTTCATCTGGGCCATTCTTCAGAACAAGAAGGAACTCTCCAAGGTCATCTGGGAGCAAA
CCAAAGGCTGTACTCTGGCCGCCTTGGGGGCCAGCAAACTTCTGAAGACCCTGGCCAAAGTTAAGAATGA
TATCAACGCAGCTGGGGAATCTGAGGAACTGGCTAATGAGTATGAGACCCGAGCAGTGGAGTTGTTCACT
GAGTGTTACAGCAGTGATGAGGACTTGGCAGAACAGCTACTGGTCTACTCTTGTGAAGCCTGGGGTGGGA
GCAACTGTCTGGAGCTGGCGGTGGAGGCTACGGACCAGCATTTCATTGCTCAGCCTGGGGTCCAGAATTT
CCTTTCTAAGCAATGGTATGGAGAGATTTCCCGAGACACGAAGAACTGGAAGATTATCCTGTGTCTGTTC
ATCATCCCCCTGGTGGGCTGTGGCCTCGTATCGTTTAGGAAGAAGCCCATTGACAAGCACAAGAAGCTGC
TCTGGTACTACGTGGCCTTCTTCACTTCGCCCTTCGTGGTCTTCTCCTGGAACGTGGTCTTCTACATCGC
CTTCCTCCTGCTGTTTGCGTATGTGCTGCTCATGGACTTCCACTCGGTGCCACACACCCCCGAGCTGATC
CTCTATGCCCTGGTCTTCGTCCTCTTCTGTGATGAAGTGAGGCAGTGGTACATGAACGGAGTGAATTATT
TCACCGACCTATGGAACGTTATGGACACACTGGGACTTTTCTACTTCATAGCGGGTATTGTATTCCGGCT
TCACTCTTCAAATAAAAGCTCTTTGTACTCCGGGCGAGTCATTTTCTGTCTGGATTACATTATATTCACT
CTAAGGCTCATCCACATTTTCACCGTGAGCAGGAACCTGGGACCCAAGATTATAATGCTGCAGCGGATGC
TCATCGACGTTTTCTTCTTCTTGTTTCTCTTTGCTGTGTGGATGGTGGCCTTCGGCGTAGCCAGACAGGG
GATCCTTAGGCAAAATGAACAGCGCTGGAGGTGGATCTTCCGCTCTGTCATCTATGAGCCCTACCTGGCC
ATGTTTGGCCAGGTGCCCAGTGATGTGGACAGTACCACATATGACTTCTCCCACTGCACCTTCTCGGGAA
ATGAGTCCAAGCCACTGTGCGTGGAGCTAGATGAATACAATCTGCCCCGCTTCCCTGAGTGGATCACCAT
CCCACTAGTGTGCATCTACATGCTCTCCACCAACATCCTTCTGGTCAATCTCCTGGTCGCCATGTTTGGC
TACACGGTGGGCATTGTGCAGGAGAACAACGATCAGGTCTGGAAGTTCCAGCGGTACTTCCTGGTGCAGG
AGTACTGCAACCGCCTCAACATCCCCTTCCCCTTCGTCGTCTTCGCTTACTTCTACATGGTGGTCAAGAA
GTGTTTCAAATGCTGCTGTAAAGAGAAGAACACGGAGTCTTCTGCCTGCTGTTTCAGAAATGAGGACAAC
GAGACTTTGGCGTGGGAGGGCGTCATGAAGGAGAATTACCTTGTCAAGATCAACACGAAGGCCAACGACA
ACGCAGAGGAGATGAGGCATCGGTTCAGACAACTGGACACAAAGCTTAATGATCTCAAAGGTCTTCTGAA
AGAGATTGCTAATAAAATCAAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_134371
Insert Size 3315 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_134371.2, NP_599198.2
RefSeq Size 4184 bp
RefSeq ORF 3315 bp
Locus ID 171384
UniProt ID Q8R455
Gene Summary excitatory ion channel; may be involved in transducing cold stimuli [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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