Adgrl1 (NM_022962) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CL1BA; Lphn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211905 representing NM_022962 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTTGGCTGCAGCACTCTGGAGTCTCTGTGTGACGACTGTCCTCGTCACCTCTGCTACCCAAG GCCTGAGCCGGGCTGGACTCCCATTTGGATTGATGCGCCGGGAGCTAGCATGCGAAGGCTACCCCATTGA GCTGCGGTGCCCGGGCAGTGACGTCATCATGGTGGAGAATGCAAACTATGGGCGCACAGATGACAAGATC TGCGATGCCGACCCTTTTCAGATGGAGAACGTGCAGTGCTACCTGCCTGACGCCTTCAAAATCATGTCAC AGAGATGTAATAACCGAACCCAGTGTGTGGTGGTGGCCGGCTCTGACGCCTTTCCTGACCCCTGTCCTGG AACCTACAAGTACCTGGAGGTGCAGTACGACTGTGTCCCTTACAAAGTGGAGCAGAAAGTCTTCGTGTGC CCAGGGACACTGCAGAAGGTGCTGGAGCCCACCTCCACACATGAATCGGAGCACCAGTCTGGCGCATGGT GCAAGGACCCACTGCAGGCAGGTGACCGTATCTACGTTATGCCCTGGATCCCCTACCGCACGGACACACT GACCGAGTATGCTTCCTGGGAGGACTATGTGGCTGCACGCCACACCACCACGTACAGACTGCCCAACCGT GTAGATGGCACTGGCTTTGTGGTATATGATGGTGCCGTCTTCTATAACAAGGAACGTACTCGCAACATTG TCAAATATGACCTGCGGACCCGCATCAAGAGCGGAGAAACAGTCATAAACACAGCCAACTACCACGACAC CTCACCTTATCGCTGGGGAGGCAAAACCGACATTGACCTGGCAGTGGATGAGAACGGGCTGTGGGTCATC TATGCCACCGAGGGGAACAACGGGCGTCTGGTGGTGAGCCAGCTCAACCCCTACACACTGCGTTTCGAGG GCACCTGGGAAACAGGCTATGACAAGCGCTCAGCCTCCAATGCCTTCATGGTGTGTGGTGTCCTCTATGT GCTGCGCTCTGTTTATGTGGATGACGACAGTGAGGCAGCAGGCAACCGCGTGGACTATGCCTTTAACACC AATGCAAACCGAGAGGAGCCCGTCAGTCTCGCCTTCCCCAACCCCTACCAGTTTGTATCTTCTGTTGACT ACAATCCCCGGGACAACCAGCTGTATGTGTGGAACAACTATTTCGTGGTGCGCTACAGCCTGGAGTTTGG ACCCCCAGATCCCAGTGCTGGCCCAGCCACTTCCCCACCTCTCAGTACCACCACCACAGCTCGGCCTACG CCCCTCACCAGCACAGCCTCACCTGCAGCCACCACTCCACTCCGCCGGGCGCCCCTCACCACGCACCCAG TAGGTGCCATCAACCAGCTGGGACCTGACCTGCCTCCAGCCACAGCCCCAGCACCCAGTACCCGGCGGCC TCCAGCCCCCAATCTGCATGTGTCCCCTGAGCTCTTCTGTGAACCCCGAGAGGTCCGGCGGGTCCAGTGG CCAGCTACCCAGCAGGGTATGCTGGTAGAGAGACCTTGCCCCAAGGGAACTCGAGGAATTGCCTCGTTCC AGTGCCTCCCAGCTCTGGGGCTCTGGAATCCTCGGGGCCCTGACCTCAGCAACTGCACTTCCCCCTGGGT CAACCAAGTCGCCCAGAAGATCAAGAGTGGAGAGAATGCAGCCAACATTGCTAGTGAGCTGGCCCGCCAC ACGCGGGGCTCCATCTATGCTGGGGACGTGTCCTCATCGGTGAAGCTGATGGAGCAACTGCTAGATATCC TGGATGCCCAGCTCCAGGCCCTACGGCCCATTGAACGAGAGTCAGCTGGCAAGAACTACAATAAGATGCA CAAGCGAGAGAGAACCTGCAAGGACTATATCAAGGCTGTGGTGGAGACAGTGGACAACCTGCTTCGGCCA GAGGCACTTGAGTCATGGAAAGACATGAATGCCACCGAACAGGTCCATACGGCCACCATGCTCCTAGATG TCTTAGAGGAGGGTGCCTTCCTGCTGGCCGACAATGTCAGAGAACCTGCTCGCTTCTTGGCTGCCAAGCA GAATGTGGTCCTGGAGGTCACTGTCCTGAGCACAGAGGGTCAAGTGCAGGAGTTGGTGTTCCCCCAGGAG TATGCCAGTGAGAGCTCCATTCAGCTGTCCGCCAACACCATCAAGCAGAACAGCCGCAATGGTGTGGTGA AGGTTGTCTTCATTCTCTACAACAACCTGGGCCTCTTCTTGTCCACGGAGAATGCCACAGTGAAGCTGGC AGGTGAGGCAGGGACCGGTGGCCCTGGAGGTGCCTCCCTGGTGGTTAACTCACAGGTCATCGCAGCATCC ATCAATAAGGAGTCCAGCCGTGTCTTCCTCATGGACCCTGTCATCTTTACTGTGGCCCACTTGGAGGCCA AGAACCACTTCAATGCAAACTGCTCCTTCTGGAACTACTCAGAGCGCTCCATGCTGGGCTACTGGTCAAC CCAGGGCTGCCGACTGGTGGAGTCCAATAAGACCCATACCACATGTGCCTGCAGCCACCTCACCAACTTC GCAGTGCTCATGGCTCACCGAGAGATCTACCAAGGCCGTATTAATGAGCTGTTGCTGTCAGTCATCACCT GGGTTGGCATTGTCATCTCCCTGGTCTGTCTGGCTATCTGCATCTCCACCTTCTGCTTCCTGCGGGGCCT GCAGACCGACCGCAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTTGCAGAGCTGCTCTTCCTG GTTGGAATAGACAAAACTCAGTATGAGGTCGCCTGCCCTATCTTTGCGGGCCTGCTGCACTACTTCTTCC TGGCCGCCTTCTCCTGGCTGTGCCTAGAGGGCGTGCACCTCTACCTCCTGCTGGTCGAGGTGTTCGAGAG CGAATATTCACGCACCAAGTACTATTACCTGGGCGGCTACTGCTTCCCAGCCCTGGTGGTAGGCATCGCA GCCGCCATTGACTACCGAAGCTACGGCACTGAGAAGGCCTGCTGGCTGAGGGTGGATAACTATTTCATCT GGAGCTTCATTGGGCCCGTCTCCTTTGTTATTGTGGTGAACCTGGTGTTCCTCATGGTGACCCTGCACAA GATGATCCGAAGCTCATCCGTGCTCAAGCCTGACTCCAGCCGCCTTGACAACATCAAGTCCTGGGCGCTG GGTGCCATTGCACTGCTCTTCCTGCTGGGCCTCACCTGGGCTTTCGGCCTCCTCTTCATCAACAAGGAGT CAGTAGTAATGGCTTACCTCTTCACAACCTTCAACGCCTTCCAGGGGGTCTTCATCTTTGTCTTTCACTG CGCCTTACAGAAAAAGGTGCACAAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATTCGCTCC CCACCTGGGGGGGCTCACGGCTCCCTTAAGACCTCAGCCATGCGAAGTAACACCCGCTACTACACAGGGA CCCAGAGCCGAATCCGGAGGATGTGGAATGACACCGTGAGGAAGCAGACAGAGTCGTCCTTTATGGCAGG GGACATCAACAGCACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTACTGACCAACCCTGTGCTA CAGCCCCGTGGGGGCACTAGCCCATACAATACACTCATTGCAGAGTCTGTGGGCTTCAATCCCTCCTCGC CCCCAGTCTTCAACTCCCCAGGAAGCTACAGGGAACCTAAGCACCCCTTGGGCGGCCGGGAAGCCTGTGG CATGGACACACTGCCCCTTAATGGCAACTTCAACAACAGCTACTCCTTGCGAAGTGGTGATTTCCCTCCG GGGGATGGGGGTCCTGAGCCACCCCGAGGCCGAAACCTAGCGGATGCTGCGGCCTTTGAGAAGATGATCA TCTCAGAGCTGGTGCACAACAACCTTCGGGGGGCCAGTGGGGGCGCCAAAGGTCCTCCACCAGAGCCTCC TGTGCCACCCGTGCCAGGAGTCAGTGAGGACGAGGCTGGTGGGCCTGGGGGTGCTGACCGGGCTGAGATT GAACTTCTCTACAAGGCCCTGGAGGAGCCACTGCTGCTGCCCCGGGCCCAGTCGGTGCTGTACCAGAGTG ATCTGGATGAGTCGGAGAGCTGTACGGCAGAGGATGGGGCCACCAGCCGGCCCCTCTCCTCCCCTCCCGG CCGGGACTCCCTCTATGCCAGCGGGGCCAACCTGCGGGACTCGCCCTCCTACCCGGACAGCAGCCCCGAA GGGCCTAATGAGGCCCTGCCCCCTCCCCCACCTGCTCCCCCTGGGCCCCCAGAAATCTACTACACCTCTC GCCCGCCGGCCCTGGTGGCTCGGAATCCCCTACAGGGCTACTACCAGGTGCGGCGGCCCAGCCATGAGGG CTACCTGGCAGCCCCCAGCCTTGAGGGGCCAGGGCCCGATGGGGATGGGCAAATGCAGTTGGTCACTAGT CTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022962
Insert Size	4416 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022962.1, NP_075251.1
RefSeq Size	5579 bp
RefSeq ORF	4416 bp
Locus ID	65096
UniProt ID	O88917
Gene Summary	G protein-coupled receptor for alpha-Latrotoxin [RGD, Feb 2006]

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