Gpx3 (NM_022525) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Synonyms	GPx-3; GPx-P; Gpxp; GSHPx-3; GSHPx-P
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211963 representing NM_022525 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGATCCTTCGGGCATCCTGCCTCCTGTCCCTGCTCCTGGCCGGGTTTGTTCCGCCGGGCCGGG GACAAGAGAAGTCCAAGACAGACTGCCATGGCGGTATGAGCGGTACCATCTACGAGTATGGAGCCCTTAC CATCGATGGGGAGGAATACATTCCTTTTAAGCAGTATGCTGGCAAATACATCCTCTTTGTCAACGTAGCC AGCTACTGAGGTCTGACAGACCAATACCTTGAACTGAATGCACTACAAGAAGAACTTGGCCCATTCGGCC TGGTCATTCTGGGCTTCCCTTGCAACCAATTTGGCAAACAGGAGCCAGGCGAGAACTCGGAGATCCTGCC TAGTCTCAAGTACGTTCGACCGGGTGGGGGCTTTGTGCCTAATTTCCAGCTCTTTGAGAAAGGAGACGTG AACGGGGAGAAAGAGCAGAAGTTCTACACTTTCCTGAAGAACTCCTGCCCTCCCACTGCGGAACTCCTGG GCTCACCTGGCCGCCTCTTTTGGGAACCCATGAAGATCCATGACATCCGCTGGAACTTTGAGAAGTTCCT GGTGGGGCCAGATGGCATACCAATTATGCGCTGGTACCACCGGACCACAGTCAGCAACGTCAAGATGGAC ATCCTGTCCTATATGAGGCGGCAGGCAGCCCTGGGGGCCAGAGGGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022525
Insert Size	681 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022525.3, NP_071970.2
RefSeq Size	1488 bp
RefSeq ORF	681 bp
Locus ID	64317
UniProt ID	P23764
Gene Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted and is predominantly expressed in rat kidney, which appears to be the major source of the enzyme in plasma. Dysregulation of this isozyme has been associated with obesity-related metabolic complications. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Aug 2016]

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