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Mefv (NM_031634) Rat Untagged Clone
CAT＃: RN212057
Mefv (untagged ORF) - Rat Mediterranean fever (Mefv), (10 ug)
CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | pyrin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN212057 representing NM_031634
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAATACCCGTGTTGACCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGAACTAGAGAAAT TCAAGTTTAAGTTGCACACCACCAGCCTGGAGAAGGGCCACTCTAGGATCCCCCTGAGCCTCGTGAAGAT GGCCAGGCCAATAAAGCTGACCAGACTCCTGCTAACCTACTATGGGGAAGAATATGCTGTAAGGCTGACC CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCACAAGGCCACAGGCCCAGAAC ATTTGACTGAAGAAAATGGAGTTGGCGGTTCTGTGCAGTCTTCTGCAGAGAATAAGGACAAGGGTGTGAA GGGATCAGATGTCCTTGGAGAGGATGAGGCACAGCAGAACGATGATGAATCAGACATCCTGCCACCCATC CAGGCAGAAGTGGGGAAGGGGCCCCAGAAGAAATCACTGGCCAAAAGGAAGGATCAGAGGGGCCCTGAGA GCCTGGACTCACAGACCAAGCCAGGGGCCAGGAGTGCAGCACCACTCTATAGGAGAACCCTGGTGACCCA GTCCCCAGGGGACAAGGAGAACAGAGCGGGTGCTCAGACTCCGCAGAATGCCCAGCTCTGCAGGGAGGCT GCAAGGAACTCTACAGCAATGTCCCAGGGAGGAGAGAGATCAAGAAGGCTGAAGTGTATTTGCCTTCAGG AAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTACTCAAAAGAAGGAGAACCCCCAAATTCAGAAGCT CTTCCGACTCAAGAGGAAACAAGAAATGGCAGTCTCATTCGTACGAGAAACAGCCACCCTGAATGGAAGG ACTACGGGGACTCTGGAAAAGGGTGTAGGGATTCCAGAACATTCCATGATGCTGGATGAAGAAACATCCA GAAACATGTCTTCCAAAATATCGTTGACTAGGGAGAAGAGATGCACTGCGTCATGGACAGAAAATGGAAA CGGGGGTCCAGAAACCCCAGAGACCTTGGGAGAGACAGTCAGCAGCATACTCTGTGACTCCTGCAGTCCA AAAGTGCTGCTGTCATTAGGTGAAAAACTGGCCCAGACTCCAGAAGACCCAGCATCTTTAGGACAGGCAG CTTCTAAAGGAAGGTCACGGGACAAGGTTGCATGCCCTCTTTGCCACACCCAAGGAGAACTGCCTGCTAA GGCCTGTGTGCAAAGTTCCTGTAGCTGCTCGGTTGCTCCTGGGGACCCCAAGGCCTCAGGCAGATGCTCC ATATGCTTCCAGTGCCAAAGCTCGCGTGCAGGAAAGAGCTGTGAAGCCCAGAGCCCCCAGTTCCTACCAC AGTGCCCACGTCACATGAAGCAGGTGCAGCTGCTTTTCTGTGAGGACCACAGGGAGCCCATCTGCCTCAT CTGCAGGCTGAGCCAGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGGCTGCACTACAGTACAAG GAGCAGATCCGGAAGCAGTTGGAGCGCCTGCGNGAGATGAGGGGATATGTGGAGGAGCACAAGCTTCCGG CAGACAAGAAAGCAGAGGACTTCCTGAAACAAACAGAAACTCAGAAACAGAGGATATCATGTCCGCTTGA GAAGCTATTCCAATTCCTGGAGCAGCAAGAGCAACTCTTTGTGACCTGGCTGCAGGAGCTAGTCCAGACC ATCGGCAAGGTCCGAGAGACATACTACACCCAGGTTTCCCTACTGGATAAGCTGATTGGAGAACTAGAGG CCAAGCAAGACCAGCCAGAGTGGGAGCTCATGCAGGACATTGGAGCCACCTTGCACAGGGCTGAGACGAT GACTGCCTCTGAGCTGTTGGGCATTCCTCCAGGCGTTAAAGAAAAACTGCACCTGCTCTACCAGAAATCA AAGTCCGCCGAGAAGAATATGCAGCGTTTCTCAGAAATGCTGGGCTCCGAAATGGCATTCAGCGCTTCAG ATGTAGCAACACGGGAAGGGTGCAGGCCTAGCACAACCAAGGCCCAGGCCTTGATCCCCACCGTTCACCT AAAGTGTGATGGTGCACACACACAGGATTTCGATGTAATACTTTGCGCAGAGCTGGAAGCTGGAGGATCA GAACCTCAAGATTACCTTCATCCATCGTCAGCTCAAGACACACCTGAGCTACACGAGATCCATTCTCAGA ACAACAAAAGAAAATTTAAATCTTTCCTGAAGTGGAAACCTTCCTTCTCCAGAACCGACAGGTGCTTAAG AACATGCTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031634 |
| Insert Size | 2253 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031634.1, NP_113822.1 |
| RefSeq Size | 3231 bp |
| RefSeq ORF | 2253 bp |
| Locus ID | 58923 |
| UniProt ID | Q9JJ25 |
| Gene Summary | Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR212057 | Mefv (Myc-DDK-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 9,690.00 |
|
| RR212057L3 | Lenti ORF clone of Mefv (Myc-DDK-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 11,500.00 |
|
| RR212057L4 | Lenti ORF clone of Mefv (mGFP-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 12,540.00 |
