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Mefv (NM_031634) Rat Tagged ORF Clone
CAT＃: RR212057
- TrueORF®
Mefv (Myc-DDK-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug)
"NM_031634" in other vectors (3)
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CNY 9,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | pyrin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212057 representing NM_031634
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAATACCCGTGTTGACCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGAACTAGAGAAAT TCAAGTTTAAGTTGCACACCACCAGCCTGGAGAAGGGCCACTCTAGGATCCCCCTGAGCCTCGTGAAGAT GGCCAGGCCAATAAAGCTGACCAGACTCCTGCTAACCTACTATGGGGAAGAATATGCTGTAAGGCTGACC CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCACAAGGCCACAGGCCCAGAAC ATTTGACTGAAGAAAATGGAGTTGGCGGTTCTGTGCAGTCTTCTGCAGAGAATAAGGACAAGGGTGTGAA GGGATCAGATGTCCTTGGAGAGGATGAGGCACAGCAGAACGATGATGAATCAGACATCCTGCCACCCATC CAGGCAGAAGTGGGGAAGGGGCCCCAGAAGAAATCACTGGCCAAAAGGAAGGATCAGAGGGGCCCTGAGA GCCTGGACTCACAGACCAAGCCAGGGGCCAGGAGTGCAGCACCACTCTATAGGAGAACCCTGGTGACCCA GTCCCCAGGGGACAAGGAGAACAGAGCGGGTGCTCAGACTCCGCAGAATGCCCAGCTCTGCAGGGAGGCT GCAAGGAACTCTACAGCAATGTCCCAGGGAGGAGAGAGATCAAGAAGGCTGAAGTGTATTTGCCTTCAGG AAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTACTCAAAAGAAGGAGAACCCCCAAATTCAGAAGCT CTTCCGACTCAAGAGGAAACAAGAAATGGCAGTCTCATTCGTACGAGAAACAGCCACCCTGAATGGAAGG ACTACGGGGACTCTGGAAAAGGGTGTAGGGATTCCAGAACATTCCATGATGCTGGATGAAGAAACATCCA GAAACATGTCTTCCAAAATATCGTTGACTAGGGAGAAGAGATGCACTGCGTCATGGACAGAAAATGGAAA CGGGGGTCCAGAAACCCCAGAGACCTTGGGAGAGACAGTCAGCAGCATACTCTGTGACTCCTGCAGTCCA AAAGTGCTGCTGTCATTAGGTGAAAAACTGGCCCAGACTCCAGAAGACCCAGCATCTTTAGGACAGGCAG CTTCTAAAGGAAGGTCACGGGACAAGGTTGCATGCCCTCTTTGCCACACCCAAGGAGAACTGCCTGCTAA GGCCTGTGTGCAAAGTTCCTGTAGCTGCTCGGTTGCTCCTGGGGACCCCAAGGCCTCAGGCAGATGCTCC ATATGCTTCCAGTGCCAAAGCTCGCGTGCAGGAAAGAGCTGTGAAGCCCAGAGCCCCCAGTTCCTACCAC AGTGCCCACGTCACATGAAGCAGGTGCAGCTGCTTTTCTGTGAGGACCACAGGGAGCCCATCTGCCTCAT CTGCAGGCTGAGCCAGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGGCTGCACTACAGTACAAG GAGCAGATCCGGAAGCAGTTGGAGCGCCTGCGNGAGATGAGGGGATATGTGGAGGAGCACAAGCTTCCGG CAGACAAGAAAGCAGAGGACTTCCTGAAACAAACAGAAACTCAGAAACAGAGGATATCATGTCCGCTTGA GAAGCTATTCCAATTCCTGGAGCAGCAAGAGCAACTCTTTGTGACCTGGCTGCAGGAGCTAGTCCAGACC ATCGGCAAGGTCCGAGAGACATACTACACCCAGGTTTCCCTACTGGATAAGCTGATTGGAGAACTAGAGG CCAAGCAAGACCAGCCAGAGTGGGAGCTCATGCAGGACATTGGAGCCACCTTGCACAGGGCTGAGACGAT GACTGCCTCTGAGCTGTTGGGCATTCCTCCAGGCGTTAAAGAAAAACTGCACCTGCTCTACCAGAAATCA AAGTCCGCCGAGAAGAATATGCAGCGTTTCTCAGAAATGCTGGGCTCCGAAATGGCATTCAGCGCTTCAG ATGTAGCAACACGGGAAGGGTGCAGGCCTAGCACAACCAAGGCCCAGGCCTTGATCCCCACCGTTCACCT AAAGTGTGATGGTGCACACACACAGGATTTCGATGTAATACTTTGCGCAGAGCTGGAAGCTGGAGGATCA GAACCTCAAGATTACCTTCATCCATCGTCAGCTCAAGACACACCTGAGCTACACGAGATCCATTCTCAGA ACAACAAAAGAAAATTTAAATCTTTCCTGAAGTGGAAACCTTCCTTCTCCAGAACCGACAGGTGCTTAAG AACATGCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212057 representing NM_031634
Red=Cloning site Green=Tags(s) MANTRVDHLLNTLEELLPYELEKFKFKLHTTSLEKGHSRIPLSLVKMARPIKLTRLLLTYYGEEYAVRLT LQILRATNQRQLAEELHKATGPEHLTEENGVGGSVQSSAENKDKGVKGSDVLGEDEAQQNDDESDILPPI QAEVGKGPQKKSLAKRKDQRGPESLDSQTKPGARSAAPLYRRTLVTQSPGDKENRAGAQTPQNAQLCREA ARNSTAMSQGGERSRRLKCICLQERSDPGVLKLPLTQKKENPQIQKLFRLKRKQEMAVSFVRETATLNGR TTGTLEKGVGIPEHSMMLDEETSRNMSSKISLTREKRCTASWTENGNGGPETPETLGETVSSILCDSCSP KVLLSLGEKLAQTPEDPASLGQAASKGRSRDKVACPLCHTQGELPAKACVQSSCSCSVAPGDPKASGRCS ICFQCQSSRAGKSCEAQSPQFLPQCPRHMKQVQLLFCEDHREPICLICRLSQEHQGHRVRPIEEAALQYK EQIRKQLERLXEMRGYVEEHKLPADKKAEDFLKQTETQKQRISCPLEKLFQFLEQQEQLFVTWLQELVQT IGKVRETYYTQVSLLDKLIGELEAKQDQPEWELMQDIGATLHRAETMTASELLGIPPGVKEKLHLLYQKS KSAEKNMQRFSEMLGSEMAFSASDVATREGCRPSTTKAQALIPTVHLKCDGAHTQDFDVILCAELEAGGS EPQDYLHPSSAQDTPELHEIHSQNNKRKFKSFLKWKPSFSRTDRCLRTCW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031634 |
| ORF Size | 2250 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031634.1, NP_113822.1 |
| RefSeq Size | 3231 bp |
| RefSeq ORF | 2253 bp |
| Locus ID | 58923 |
| UniProt ID | Q9JJ25 |
| MW | 84 kDa |
| Gene Summary | Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212057 | Mefv (untagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 9,690.00 |
|
| RR212057L3 | Lenti ORF clone of Mefv (Myc-DDK-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 11,500.00 |
|
| RR212057L4 | Lenti ORF clone of Mefv (mGFP-tagged ORF) - Rat Mediterranean fever (Mefv), (10 ug) |
CNY 12,540.00 |
