Scn11a (NM_019265) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NaN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212224 representing NM_019265 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGAGGAGAGGTACTACCCGGTGATCTTCCCGGACGAGCGGAATTTCCGCCCCTTCACTTCCGACTCTC TGGCTGCCATAGAGAAGCGGATTGCTATCCAAAAGGAGAGGAAGAAGTCCAAAGACAAGGCGGCAGCTGA GCCCCAGCCTCGGCCTCAGCTTGACCTAAAGGCCTCCAGGAAGTTACCTAAGCTTTATGGTGACATTCCC CCTGAGCTTGTAGCGAAGCCTCTGGAAGACCTGGACCCATTCTACAAAGACCATAAGACATTCATGGTGT TGAACAAGAAGAGAACAATTTATCGCTTCAGCGCCAAGCGGGCCTTGTTCATTCTGGGGCCTTTTAATCC CCTCAGAAGCTTAATGATTCGTATCTCTGTCCATTCAGTCTTTAGCATGTTCATCATCTGCACGGTGATC ATCAACTGTATGTTCATGGCGAATTCTATGGAGAGAAGTTTCGACAACGACATTCCCGAATACGTCTTCA TTGGGATTTATATTTTAGAAGCTGTGATTAAAATATTGGCAAGAGGCTTCATTGTGGATGAGTTTTCCTT CCTCCGAGATCCGTGGAACTGGCTGGACTTCATTGTCATTGGAACAGCGATCGCAACTTGTTTTCCGGGC AGCCAAGTCAATCTTTCAGCTCTTCGTACCTTCCGAGTGTTCAGAGCTCTGAAGGCGATTTCAGTTATCT CAGGTCTGAAGGTCATCGTAGGTGCCCTGCTGCGCTCGGTGAAGAAGCTGGTAGACGTGATGGTCCTCAC TCTCTTCTGCCTCAGCATCTTTGCCCTGGTCGGTCAGCAGCTGTTCATGGGAATTCTGAACCAGAAGTGT ATTAAGCACAACTGTGGCCCCAACCCTGCATCCAACAAGGATTGCTTTGAAAAGGAAAAAGATAGCGAAG ACTTCATAATGTGTGGTACCTGGCTCGGCAGCAGACCCTGTCCCAATGGTTCTACGTGCGATAAAACCAC ATTGAACCCAGACAATAATTATACAAAGTTTGACAACTTTGGCTGGTCCTTTCTCGCCATGTTCCGGGTT ATGACTCAAGACTCCTGGGAGAGGCTTTACCGACAGATCCTGCGGACCTCTGGGATCTACTTTGTCTTCT TCTTCGTGGTGGTCATCTTCCTGGGCTCCTTCTACCTGCTTAACCTAACCCTGGCTGTTGTCACCATGGC TTATGAAGAACAGAACAGAAATGTAGCTGCTGAGACAGAGGCCAAGGAGAAAATGTTTCAGGAAGCCCAG CAGCTGTTAAGGGAGGAGAAGGAGGCTCTGGTTGCCATGGGAATTGACAGAAGTTCCCTTAATTCCCTTC AAGCTTCATCCTTTTCCCCGAAGAAGAGGAAGTTTTTCGGTAGTAAGACAAGAAAGTCCTTCTTTATGAG AGGGTCCAAGACGGCCCAAGCCTCAGCGTCTGATTCAGAGGACGATGCCTCTAAAAATCCACAGCTCCTT GAGCAGACCAAACGACTGTCCCAGAACTTGCCAGTGGATCTCTTTGATGAGCACGTGGACCCCCTCCACA GGCAGAGAGCGCTGAGCGCTGTCAGTATCTTAACCATCACCATGCAGGAACAAGAAAAATTCCAGGAGCC TTGTTTCCCATGTGGGAAAAATTTGGCCTCTAAGTACCTGGTGTGGGACTGTAGCCCTCAGTGGCTGTGC ATAAAGAAGGTCCTGCGGACCATCATGACGGATCCCTTTACTGAGCTGGCCATCACCATCTGCATCATCA TCAATACCGTTTTCTTAGCCGTGGAGCACCACAACATGGATGACAACTTAAAGACCATACTGAAAATAGG AAACTGGGTTTTCACGGGAATTTTCATAGCGGAAATGTGTCTCAAGATCATCGCGCTCGACCCTTACCAC TACTTCCGGCACGGCTGGAATGTTTTTGACAGCATCGTGGCCCTCCTGAGTCTCGCTGATGTGCTCTACA ACACACTGTCTGATAACAATAGGTCTTTCTTGGCTTCCCTCAGAGTGCTGAGGGTCTTCAAGTTAGCCAA ATCCTGGCCCACGTTAAACACTCTCATTAAGATCATCGGCCACTCCGTGGGCGCGCTTGGAAACCTGACT GTGGTCCTGACTATCGTGGTCTTCATCTTTTCTGTGGTGGGCATGCGGCTCTTCGGCACCAAGTTTAACA AGACCGCCTACGCCACCCAGGAGCGGCCCAGGCGGCGCTGGCACATGGATAATTTCTACCACTCCTTCCT GGTGGTGTTCCGCATCCTCTGTGGGGAATGGATCGAGAACATGTGGGGCTGCATGCAGGATATGGACGGC TCCCCGTTGTGCATCATTGTCTTTGTCCTGATAATGGTGATCGGGAAGCTTGTGGTGCTTAACCTCTTCA TTGCCTTGCTGCTCAATTCCTTCAGCAATGAGGAGAAGGATGGGAGCCTGGAAGGAGAGACCAGGAAAAC CAAAGTGCAGCTAGCCCTGGATCGGTTCCGCCGGGCCTTCTCCTTCATGCTGCACGCTCTTCAGAGTTTT TGTTGCAAGAAATGCAGGAGGAAAAACTCGCCAAAGCCAAAAGAGACAACAGAAAGCTTTGCTGGTGAGA ATAAAGACTCAATCCTCCCGGATGCGAGGCCCTGGAAGGAGTATGATACAGACATGGCTTTGTACACTGG ACAGGCCGGGGCTCCGCTGGCCCCACTCGCAGAGGTAGAGGACGATGTGGAATATTGTGGTGAAGGCGGT GCCCTACCCACCTCACAACATAGTGCTGGAGTTCAGGCCGGTGACCTCCCTCCAGAGACCAAGCAGCTCA CTAGCCCGGATGACCAAGGGGTTGAAATGGAAGTATTTTCTGAAGAAGATCTGCATTTAAGCATACAGAG TCCTCGAAAGAAGTCTGACGCAGTGAGCATGCTCTCGGAATGCAGCACAATTGACCTGAATGATATCTTT AGAAATTTACAGAAAACAGTTTCCCCCAAAAAGCAGCCAGATAGATGCTTTCCCAAGGGCCTTAGTTGTC ACTTTCTATGCCACAAAACAGACAAGAGAAAGTCCCCCTGGGTCCTGTGGTGGAACATTCGGAAAACCTG CTACCAAATCGTGAAGCACAGCTGGTTTGAGAGTTTCATAATCTTTGTTATTCTGCTGAGCAGTGGAGCG CTGATATTTGAAGATGTCAATCTCCCCAGCCGGCCCCAAGTTGAGAAATTACTAAGGTGTACCGATAATA TTTTCACATTTATTTTCCTCCTGGAAATGATCCTGAAGTGGGTGGCCTTTGGATTCCGGAGGTATTTCAC CAGTGCCTGGTGCTGGCTTGATTTCCTCATTGTGGTGGTGTCTGTGCTCAGTCTCATGAATCTACCAAGC TTGAAGTCCTTCCGGACTCTGCGGGCCCTGAGACCTCTGCGGGCGCTGTCCCAGTTTGAAGGAATGAAGG TTGTCGTCTACGCCCTGATCAGCGCCATACCTGCCATTCTCAATGTCTTGCTGGTCTGCCTCATTTTCTG GCTCGTATTTTGTATCTTGGGAGTAAATTTATTTTCTGGGAAGTTTGGAAGGTGCATTAACGGGACAGAC ATAAATATGTATTTGGATTTTACCGAAGTTCCGAACCGAAGCCAATGTAACATTAGTAATTACTCGTGGA AGGTCCCGCAGGTCAACTTTGACAACGTGGGGAATGCCTATCTCGCCCTGCTGCAAGTGGCAACCTATAA GGGCTGGCTGGAAATCATGAATGCTGCTGTCGATTCCAGAGAGAAAGACGAGCAGCCGGACTTTGAGGCG AACCTCTACGCGTATCTCTACTTTGTGGTTTTTATCATCTTCGGCTCCTTCTTTACCCTGAACCTCTTTA TCGGTGTTATTATTGACAACTTCAATCAGCAGCAGAAAAAGTTAGGTGGCCAAGACATTTTTATGACAGA AGAACAGAAGAAATATTACAATGCAATGAAAAAGTTAGGAACCAAGAAACCTCAAAAGCCCATCCCAAGG CCCCTGAACAAATGTCAAGCCTTTGTGTTCGACCTGGTCACAAGCCAGGTCTTTGACGTCATCATTCTGG GTCTTATTGTCTTAAATATGATTATCATGATGGCTGAATCTGCCGACCAGCCCAAAGATGTGAAGAAAAC CTTTGATATCCTCAACATAGCCTTCGTGGTCATCTTTACCATAGAGTGTCTCATCAAAGTCTTTGCTTTG AGGCAACACTACTTCACCAATGGCTGGAACTTATTTGATTGTGTGGTCGTGGTTCTTTCTATCATTAGTA CCCTGGTTTCCCGCTTGGAGGACAGTGACATTTCTTTCCCGCCCACGCTCTTCAGAGTCGTCCGCTTGGC TCGGATTGGTCGAATCCTCAGGCTGGTCCGGGCTGCCCGGGGAATCAGGACCCTCCTCTTTGCTTTGATG ATGTCTCTCCCCTCTCTCTTCAACATCGGTCTGCTGCTCTTCCTGGTGATGTTCATTTACGCCATCTTTG GGATGAGCTGGTTTTCCAAAGTGAAGAAGGGCTCCGGGATCGACGACATCTTCAACTTCGAGACCTTTAC GGGCAGCATGCTGTGCCTCTTCCAGATAACCACTTCGGCTGGCTGGGATACCCTCCTCAACCCCATGCTG GAGGCAAAAGAACACTGCAACTCCTCCTCCCAAGACAGCTGTCAGCAGCCGCAGATAGCCGTCGTCTACT TCGTCAGTTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCTGTGATCCTCGAGAACTT CAACACAGCCACGGAGGAGAGCGAGGACCCTCTGGGAGAGGACGACTTTGAAATCTTCTATGAGGTCTGG GAGAAGTTTGACCCCGAGGCGTCGCAGTTCATCCAGTATTCGGCCCTCTCTGACTTTGCGGACGCCCTGC CGGAGCCGTTGCGTGTGGCCAAGCCGAATAAGTTTCAGTTTCTAGTGATGGACTTGCCCATGGTGATGGG CGACCGCCTCCATTGCATGGATGTTCTCTTTGCTTTCACTACCAGGGTCCTCGGGGACTCCAGCGGCTTG GATACCATGAAAACCATGATGGAGGAGAAGTTTATGGAGGCCAACCCTTTTAAGAAGCTCTACGAGCCCA TAGTCACCACCACCAAGAGGAAGGAGGAGGAGCAAGGCGCCGCCGTCATCCAGAGGGCCTACCGGAAACA CATGGAGAAGATGGTCAAACTGAGGCTGAAGGACAGGTCAAGTTCATCGCACCAGGTGTTTTGCAATGGA GACTTGTCCAGCTTGGATGTGGCCAAGGTCAAGGTTCACAATGACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-RsrII
ACCN	NM_019265
Insert Size	5298 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019265.2, NP_062138.1
RefSeq Size	5905 bp
RefSeq ORF	5298 bp
Locus ID	29701
UniProt ID	O88457
Gene Summary	Na channel alpha-subunit; may be involved in generation of electrical activity in peripheral sensory neurons [RGD, Feb 2006]

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