Plxnb3 (NM_001135878) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560615
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212366 representing NM_001135878 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGACTGACTTCCTCCAGGCTCCTGTGATGGCTCCCTGGTCTCCCTTCAGCCTCCACCTTCTGCTGC TGTTCCTGCTGTTGCTGCCCTTGACCAGGGCCCATCGCTTCTCTGTTCCGAACGCCAGCTTCAACCACCT GGTGCTGGCACCAGACCAGGGCAAACTCTATGTGGGTGCAGTGAATCACCTCTTCCAGCTCAGCCCTGAG CTGGAGATGGAGTCTGTGGCTATCACTGGCCCTGTCATTGACAGTCCTGATTGTGTGCCTTTCCGTGACC TGGCTGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAACCAGCTCTTGCTGGTAAGCAGCAGGGCACA GGAGCTGGTGGCTTGTGGACAAGTGAGGCAGGGTGTATGTGAGAAGCGGCGCCTTGGGGATGTTACTCAA GTGCTGTACCAGGCGGAGGACCCTGGTGATGGGCAGTTTGTGGCTGCCAATACCCTGGGAGTGACCACAG TAGGCCTAGTGGTGCCTTTGCCAGGCCGAGACCTCCTGCTGGTGGCCAGAGGCCTGGCAGGCAAGCTGTC AGCAGGAGTGCCACCCCTAACTGTGCGCCAGCTGGCTGGGCCACAGCCTTTCTCCAGCGAAGGTCTGGGA AGACTTGTGGTGGGTGACTTTTCAGACTATAACAATAGCTATGTAGGGGCTTTTTCTGATGCTCATTCTG CCTACTTTGTCTTCCGTCGCCGTGGGGCTCGGGCCCAGACTGAGTATCGCTCCTATGTAGCTCGCGTCTG CCTCGGAGATGTCAACCTTTATTCCTATGTGGAAGTGCCCCTTACCTGCCATGGCCAGGGTCTTATCCAG GCTGCCTTCCTTGCTCCGGACACCTTGCTGGGCGCATTTTCAGCAGGCACAAGTCAGGCACAGGCAGCCC TGTGTGCCTTTCCCTTAGCTGACCTGGACGGGAGTATGGAACAAGCCAGGCGACTTTGCTATACTACTGG TGGTCAGGGTCCCAACGGCATGGAAGAAGCCACTGTGGAGTATGGTGTCACATCCCGTTGTGTCACTCTG CCTCCTGACTCCCCCGAGTCATACCCCTGTGGTGATGAACACACCCCCAGCCCCATCGCAGGCCGCCAAC CCCTGGAGGCCCAGCCTCTGCTGCAGCTGGGACAGCCCATCAGTGCAGTGGCTGCTCTCCAGACAGATGG GCACACGATAGCCTTCCTGGGAGACACCGAGGGTCAACTACATAAGGTCTTTCTAAACAGCTCCCATGGC CAGGTATACCATTCCCAGCAAGTGGGGCCCCCAGGCTCAGCCATCAGCCCAGACCTGCTGGTAGACAACA GTGGAGACTACCTCTATGTCCTGACTGCCCAGCAGGTAGACAGGATACTTGTGGCAGCCTGCCCTCAGTT TCCTAACTGCACTACCTGCCTCCAGGCCAGGGACCCACTGTGTGGCTGGTGCATACTTCAGGGCAGGTGC ACTCGGAGGGCCGAGTGTGGGAGAGCAGTTCAGCCAAACCAGTGGCTATGGAGCTATGAAGACAACCACT GCCTTCACATCCAGAGCCTGCTACCAGCCCAGCACCCCCGTCAGGAACATGGCCAGATCACTCTGTCTGT TCCTGGGCTGCCCAACCTGGCCATGGATGAATACTTCTACTGTGCCTTTGGGGACTACAACAGTCTAGCT CAGGTGGAAGAGCACCATGTGGTCTGTGCCACCCCTCCTCAAGATCGGATGCCACCTAATCCTCCAGGCT CAGATCATGTCACCTTGCCCCTGGCCCTGATGTTTGAGGATGTGGTATTGGCTGCCACCACATTCTCCTT CTATGACTGCAGCGCTATTCAGGCCTTGGAAGTGGCTGCCCCGTGCCGCACTTGTGTGAGCAGCCTGTGG CGGTGCCACTGGTGCCCCCAGAGTAGCCACTGTGTATATGGAGAGCGCTGCCCAGAGGGTGAAAAGGCTG TCTACAGTGCCCAGGAGGTGGACATCCTGGTTCGTGGCCCAGAGGCTTGCCCCCAGGTTAAGGGTCTAGC AAGTCCCCAGTTGGTGCCTGTGGGCTGGGAGAGCCATGTGACCCTGCACATTGAGAACCTTCACTATTTC CGAGGTCTGCCTGCCTTGTACTATTGCTGGCTAGAACTGCCTGGAAAACTGCGAAAGCTACCTGCCTTCC TAGAGGAGACATCCAGGAACTCTGGCCTCATTCACTGCCAGGCCCAGCAGTTCCACCCCTCTATGTCTCA GTGGGAGCTCCCAGTGCCCATCTATGTAACCAGGGGTGAGATCCAGCGGCTGGACAATACCGGTGATCTT CATGTGACTCTATATGACTGTGCCATGGGTCACCCTGACTGCAGCCACTGTCAGGCAGCCAATGGGAGCC TGAGCTGCTTGTGGTGTGGTGATGGCCAGCCTGCCTGCCGCTACGGGCCGCTGTGCCCACCAGGTGCTGT GGAACAGCTATGCCCTATACCCAGCATTGATGTGATTGAACCCCTGACTGGTCCCCCAGAAGGTGGCTTA GCCATCACCATCCTGGGATCCAACCTGGGCCAGGCCTTTAATGATGTGCGAAATGCTGTGACTGTGGCTG GCCAGCCCTGCAACCCTGACCCTTCTCTCTACCGCATCTCTGCCCGGATTGTATGTGTCACATCTCCTGC TCCCAATGGCACTTCTGGACCAGTCCAAGTGGCCATTAAGAGTCGTCCTCCTGGCATCTCGGCCCAGCAC TTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAATCCTCAGTGGGGCCCCCAGGCAGGGGGCACCCAGC TCACCATTCATGGGCAGTACCTCCAGACAGGAGGCAACGTCAGTGCTTTTGTGGGGGACCAACCTTGCCC TATTCAAGAGCCTGTGTGTCCTGAAGCCATCATATGCCACACCATGCCCCAGATGGAGCCAGGAGAAGCA GTGGTCTTCGTAGTCTTTGGCCATGTTGAACGCAAACTGCTCACCACTCCTTTTCGATACACTGCCAACC CCCAACTGGTGGAAGCAGAGCCTAGTGTCAGCTTCCGAGGAGGTGGACGAGTGATTCGAGTTAGGGGTAC AGGCTTAGATGTTGTGTGGCAGCCCTTGCTGTCTGTGTGGCTGGAGGATGAGTTCCAAGTGAAGGCTTTG GGCGTGCAGGCCCAGGATGTGAACCCAAGGAGGAGCTGTGGTGCCCCTGCTGCAGATCCTCAGGCTTGTA TCCATCTTGAGAGTGGCTTACTACAGTGCTCCACTCTCTGCTCTGTCAACTCATCTAGTCTCCTCCTGTG CCACAGTCCAGCGGTGCCAGATGGGGCCCTCCCAAAGCGAGTCTTCTTTGCCCTAGACAACATGCAAGTA GACTTTGCCAGTGCTAGTGGGGGCCAGGGATTCCTCTACCAGCCTAACCCTCGCCTGGCTCCACTTAGTC ATGAGGGGATTACTCACCCCTATCGGCTCAAGCCAGGTCATGTCCTGGATGTGGAGGGCGAGGGCCTCAA TCTGGGGATCAGCAAGGAGGAGGTGCAGGTGCATATTGGAGATGGGAAGTGCCTGGTGAAGACGCTGACG CTTACTCACTTGTACTGTGAACCACCCCAGCAAGCCCCTCAGCCCACCAATGGTTCAGGCACCCTGCCAC AGTTTGTGGTGCAGATGGGCAATGTGCGCCTGGCTCTAGGCCCTGTTCAGTATGAAGCAGAGCCCATGAT ATCCACCTTTCCTGTGGAGGCCCAGGTGGGCCTGGGCATGGGTGCTGCCATGCTGATTGCTGCAGTGCTA CTCCTCACCCTCATGTACAGGCACAAGAGCAAGCAGGCCTTGCGAGACTATCAGAAGGTACTGGTGCAGT TGGAGAACCTGGAGACTGGTGTGGGTGACCAGTGCCGCAAGGAGTTCACAGACCTGATGACTGAGATGAC AGACCTCACCAGTGACCTAGAGGCCAGTGGGATTCCCTTCCTGGATTACCGAACATATGCTGAGCGTGCT TTCTTCCCTGGCCATGTTGGCTGCCCACTGCAGCCAGGGCTCGAGGGGCCTGGGGAAGAGGGTCGCCGTG TCACCGTGTGCCAGGGTCTCACACAACTCTCCAATTTGCTCAACAGCAAGCTCTTCCTTCTCACACTCAT CCACACCTTAGAGGAGCAGCCAAGCTTTTCCCAGAGGGACCGCTGCCATGTGGCTTCACTACTTTCTCTG GCTCTGCACAGCAAGCTTGAATATTTGACCGACATCATGAGAACTCTGCTTGGTGACCTAGCAGCCCATT ATGTACACAAGAACCCCAAGCTCATGTTACGCAGGACAGAGACCATGGTAGAGAAGCTGCTCACCAACTG GCTATCTATCTGTCTCTACGCCTTCTTGAAGGAAGTGGCTGGTGAGCCACTGTACATGCTCTTCCGGGCC ATCAAGTACCAGGTGGACAAGGGCCCTGTGGACGCTGTGACAGGCAAGGCAAAACGAACTCTGAATGACA GCCACCTACTACGAGAGGATGTGGAGTTCCGGCCCCTGACCCTGATGGCATTGGTGGGACCAGGGGCTGG TGGGGCAGCAGGAAACAGTGAAGTACATCGAGTGCCAGCCCGGGTGCTTGATACAGACACCATCACCCAA GTCAAAGAGAAGGTGTTGGATCAAATCTACAAGGGAACTCCCTTCTCCCAGAGGCCTTCAGTGCACTCCC TAGACCTTGAGTGGCGCTCAGGCCTAGCTGGTCACCTAACCTTGTCAGATGAGGATTTGACCTCTGTGAC TCAAAACCACTGGAAGAGACTCAACACCCTACAACACTACAAGGTCCCAGATGGAGCAACAGTGGTACTC ATTCCTCAGCTGCACAATGGTGGCACTGTCTCCCAGAGTCTGGAGCAGACTGGCTGCCATTCTGGAGAGA ACACTCCCATGCTGGAAGATGGTGAAGAAGGTGGAGTTCGGCTTTGGCACCTGGTAAAGGCCACTGAGGA GACAGAAGGGGCCAAAGTGCGGCGCAGCAGCCTCCGGGAACGAGAACGAGAACGGGCACGAGCCAAGGCC ATTCCTGAAATCTATCTTACTCGCTTACTTTCAATGAAGGGCACCCTGCAGAAGTTTGTGGATGACACCT TCCAGGCCATTCTTAGCATGAATAGGCCTGTGCCTATTGCTGTTAAGTACCTATTTGACTTCTTGGATGA GCTGGCAGAGAAACATGGCATTGAAGATCCAGAGACCCTGCACATTTGGAAGACAAACAGCTTATTGCTG CGATTCTGGGTGAATGCCTTGAAGAACCCACAGCTCATCTTTGATGTTCGAGTGTCAGACAACGAGGATG CCATCTTGGCTGTCATCGCACAGACCTTCATCGATTCCTGCATGGTCTCAGAGCATAAAGTGGGCCGGGA TTCCCCAGTGAACAAACTGCTCTATGCCCGGGAGATCCCTCGCTACAAGCAGATGGTGGAGAAGTACTAT GCGGACATTCGCCAGAGCTCTCCAGCGAGCTATCAAGAGATGAACTCAGCTCTGGCTGAACTCTCTGGGA ATTATACTTCTGCTCCCAACTGTCTGGAGGCTCTTCGAGAACTTTACAACCACATCCACAGGTATTATGA CCAGATCATCAGTGCCCTGGAGGAGGACCCCGTGGCCCAGAAGATGCAGCTGGCTTGCCGCCTGCAGCAG GTCGCTGCCTTGGTGGAGTACAAAGTGACTGATTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135878
Insert Size	5709 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135878.1, NP_001129350.1
RefSeq Size	5950 bp
RefSeq ORF	5709 bp
Locus ID	363517
UniProt ID	D3ZLH5
Gene Summary	Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca(2+)/Mg(2+)-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.