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Map3k7 (NM_001107920) Rat Untagged Clone
CAT＃: RN212444
Map3k7 (untagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug)
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Tak1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN212444 representing NM_001107920
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACAGCATCCGCCGCCTCGTCCTCCTCCTCGTCTTCTGCCAGTGAGATGATCGAAGCGCCATCGC AGGTCCTTAACTTCGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGGAGAGGAGCTTT TGGAGTGGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCAATTAAACAGATTGAAAGTGAATCTGAG AGGAAGGCTTTCATCGTGGAGCTCCGGCAGTTGTCCCGTGTGAACCATCCTAATATTGTAAAGTTGTATG GAGCCTGCTTGAATCCAGTCTGTCTTGTGATGGAGTATGCTGAAGGAGGCTCGTTGTATAATGTGCTGCA CGGTGCTGAACCATTGCCTTATTACACTGCTGCTCATGCCATGAGCTGGTGTCTACAGTGTTCCCAAGGA GTGGCCTACCTGCACAGCATGCAGCCCAAAGCTCTCATTCATAGGGACCTCAAGCCTCCAAACTTGCTGC TGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAGACACACATGAC CAATAATAAAGGGAGTGCTGCTTGGATGGCACCCGAAGTGTTCGAAGGTAGCAATTACAGTGAAAAGTGT GATGTCTTCAGCTGGGGTATTATCCTTTGGGAGGTGATAACACGCCGGAAACCCTTTGATGAGATCGGTG GCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGTACTCGACCACCACTGATCAAAAACTTGCCTAA GCCCATTGAGAGCTTGATGACCCGCTGTTGGTCTAAGGACCCTTCTCAGCGCCCTTCAATGGAGGAAATT GTGAAAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCTGATGAGCCGTTACAGTACCCCTGTCAGT ACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTTATGGACATCGCTTCTACAAATAC CAGTAATAAAAGTGACACAAATATGGAACAAGTCCCTGCCACAAACGACACTATTAAACGCTTGGAGTCA AAACTGTTGAAAAACCAGGCAAAGCAACAGAGTGACTCTGGACGCCTGAGTTTGGGAGCCTCTCGAGGGA GCAGTGTGGAGAGCTTGCCCCCGACTTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGC GAGGATCGTGGCGACTACAGCCTATACCAAGCCTAAACGGGGCCACCGTAAAACCGCTTCATTTGGCAAC ATTCTGGATGTCCCTGAGATCGTCATATCAGGCAACGGGCAACCAAGGCGTAGATCCATCCAAGACTTGA CTGTTACTGGGACAGAACCTGGTCAGGTGAGCAGCAGGTCATCCAGCCCTAGTGTCAGAATGATCACTAC CTCAGGACCAACCTCAGAGAAGCCAGCTCGCAGTCTTCCTTGGACCCCTGATGATTCCACAGATACCAAT GGCTCGGATAACTCCATCCCAATGGCGTATCTTACACTGGATCACCAGCTGCAGCCTCTAGCACCATGCC CAAACTCCAAAGAATCCATGGCAGTGTTCGAACAGCATTGTAAAATGGCACAGGAATATATGAAAGTTCA AACCGAAATCGCATTGTTACTACAGAGGAAGCAAGAACTAGTTGCAGAATTGGACCAGGATGAAAAGGAC CAGCAAAATACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTAGACGAAAACAAAAGCCTTTCTACTT ATTACCAGCAATGCAAAAAACAACTAGAGGTCATCAGAAGCCAGCAGCAGAAACGACAAGGCACTTCATG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001107920 |
| Insert Size | 1821 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001107920.2, NP_001101390.2 |
| RefSeq Size | 4540 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 313121 |
| UniProt ID | P0C8E4 |
| Gene Summary | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (By similarity). Promotes TRIM5 capsid-specific restriction activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR212444 | Map3k7 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 6,180.00 |
|
| RR212444L3 | Lenti ORF clone of Map3k7 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 7,980.00 |
|
| RR212444L4 | Lenti ORF clone of Map3k7 (mGFP-tagged ORF) - Rat mitogen activated protein kinase kinase kinase 7 (Map3k7), (10 ug) |
CNY 8,840.00 |
