Met (NM_031517) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hgfr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212802 representing NM_031517 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTCCCACCGCGCTGGCACCTGGCATTCTGCTGCTGCTGCTGACCTTGGCGCAGAGGAGCCATG GGGAGTGCAAGGAGGCCCTAGTGAAGTCTGAGATGAACGTGAACATGAAGTACCAGCTTCCCAACTTCAC CGCAGAAACCCCCATCCAGAATGTCGTCCTCCATGGGCACCATATTTATCTCGGAGCCACAAACTACATT TATGTTTTAAATGACAAAGACCTTCAGAAGGTATCTGAGTTCAAGACCGGGCCCGTGGTGGAACACCCAG ATTGTTTTCCTTGTCAGGACTGCAGCAGCAAAGCCAATGTGTCAGGAGGTGTTTGGAAAGACAACGTCAA CATGGCGCTGCTTGTTGACACTTACTATGACGACCAGCTCATCAGCTGTGGCAGCGTCAACAGAGGGACC TGCCAAAGGCATGTCCTTCCTCCTGACAATGCTGCCGACATTCAGTCCGAGGTTCACTGCATGTTCTCCC CACTTGCGGAGGAAGAGTCAGGCCAGTGTCCCGACTGTGTAGTGAGTGCCCTGGGAGCCAAAGTCCTCCT GTCTGAAAAGGACCGGTTCATCAATTTCTTCGTGGGGAATACGATAAACTCTTCCTACCCTCCCGATTAT TCATTGCATTCAATATCGGTGAGGCGGCTGAAGGAAACCCAGGACGGTTTTAAGTTTTTGACAGACCAGT CCTACATTGATGTCCTGGGAGAATTCCGAGATTCCTACCCCATCAAGTACATACATGCCTTCGAAAGCAA CCATTTTATCTACTTTCTGACTGTCCAGAAGGAAACCCTAGATGCTCAGACTTTCCATACAAGAATAATC AGGTTCTGTTCTGTAGACTCTGGGTTGCACTCCTACATGGAAATGCCTCTGGAGTGCATTCTGACGGAAA AAAGAAGAAAGAGATCCACAAGGGAAGAAGTGTTTAATATCCTCCAAGCCGCGTATGTCAGTAAACCAGG GGCCAATCTTGCTAAGCAAATAGGGGCCAGCCCGTATGATGACATTCTCTACGGGGTGTTTGCACAAAGC AAGCCAGATTCTGCTGAGCCCATGAACCGATCAGCGGTCTGTGCATTCCCCATCAAATATGTCAATGACT TCTTCAACAAGATTGTCAACAAAAACAACGTACGGTGTCTCCAGCATTTTTATGGACCCAACCACGAGCA CTGTTTCAATAGGACCCTGCTGAGAAATTCATCGGGCTGCGAAGTGCGCAGTGACGAGTACCGGACGGAG TTTACCACAGCGCTGCAGGCTGTGGATTTATTCATGGGCCGGCTCAACCATGTACTCTTGACGTCTATCT CTACCTTCATCAAAGGTGACCTCACCATTGCTAATCTAGGGACATCAGAAGGTCGCTTCATGCAGGTGGT GCTCTCTCGCACAGCACATTTCACCCCCCATGTGAATTTCCTCCTGGATTCCCATCCTGTGTCTCCGGAA GTTATTGTCGAACATCCATCAAATCAAAATGGCTATACCCTGGTGGTCACAGGGAAGAAGATCACCAAGA TTCCACTGAATGGCCTAGGCTGTGGGCATTTCCAGTCCTGCAGTCAGTGTCTCTCTGCCCCCTACTTTAT ACAGTGTGGCTGGTGCCACAATCGGTGTGTGCATTCCAATGAATGCCCCAGCGGTACATGGACTCAAGAG ATCTGTCTGCCAGCAGTTTATAAGGTTTTCCCCACTAGTGCACCCCTCGAAGGAGGAACAATGCTGACCA TATGTGGCTGGGACTTTGGATTCAAGAAGAATAATAAATTTGATTTAAGGAAAACCAAAGTTCTGCTTGG CAACGAGAGCTGTACCTTGACCTTAAGCGAGAGCACGACAAATACGTTGAAATGCACAGTTGGCCCCGCG ATGAGTGAGCACTTCAATGTGTCTGTGATCGTCTCAAACAGTCGAGAGACAACACAGTACAGTGCGTTTT CCTATGTGGATCCTGTAATAACAAGTATTTCTCCAAGGTATGGTCCTCATGCCGGAGGCACCTTACTCAC TTTGACTGGAAAATACCTCAACAGCGGCAATTCTAGACACATTTCAATCGGAGGGAAAACATGTACTTTA AAAAGTGTATCAGATAGCATTCTCGAATGCTACACCCCAGGCCACACCGTCTCTGCCGAGTTTCCCGTGA AATTGAAAATCGACCTGGCTGACCGAGTGACAAGCAGCTTCAGTTACGGGGAAGACCCGTTTGTCTCTGA AATCCACCCGACCAAATCTTTTATCAGTGGTGGAAGCACAATAACGGGGATTGGAAAGAACCTGAATTCA GTTAGCACCCCAAAGCTGGTAATAGAAGTGCATGACGTGGGCGTGAACTACACCGTGGCGTGCCAACATC GCTCGAGTTCAGAGATCATCTGCTGCACCACTCCTTCCCTGCAACAGCTGGACCTGCAACTCCCCCTGAA GACCAAAGCCTTCTTCCTGCTGGACGGGATCCTTTCCAAACACTTTGATCTCACTTATGTACATGATCCT ATGTTTAAGCCTTTTGAAAAGCCAGTAATGATCTCCATGGGCAATGAGAATGTAGTGGAAATTAAGGGAG ACGATATTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTCGGGAATAAGAGCTGTGAGAATCTCCA CTGGCATTCTGAAGCTTTGTTGTGTACGGTCCCCAGTGACCTGCTGAAGCTGAACGGCGGCGAGCTAAAT ATAGAGTGGAAGCAAGCAGTCTCTTCAACTGTCCTTGGAAAAGTGATCGTTCAACCGGATCAGAATTTTG CAGGATTGATCATTGGTGCGGTCTCAATATCAGTGGTAGTTTTGTTAGTATCCGGGCTCTTCCTGTGGCT GAGAAAGAGAAAGCATAAAGATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCCTCAT TTGGATAGGCTTGTAAGTGCCCGAAGTGTAAGCCCAACTACAGAGATGGTCTCAAATGAGTCTGTAGACT ACAGAGCTACTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCCTGCAGACAAGTGCAGTA TCCACTGACAGATCTGTCCCCCATCCTGACGAGTGGAGACTCTGATATATCCAGCCCATTACTACAAAAC ACTGTTCACATTGACCTCAGCGCTCTAAATCCAGAGCTGGTCCAAGCGGTGCAGCACGTAGTGATTGGAC CCAGTAGCCTGATTGTGCATTTCAATGAAGTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATGGGAC TTTGTTGGACAGTGACGGAAAGAAAATTCACTGTGCTGTGAAATCCTTGAATAGAATCACAGATATAGAA GAAGTCTCCCAGTTTCTGACTGAGGGAATCATCATGAAAGATTTCAGCCACCCCAATGTTCTCTCACTCT TGGGAATCTGCCTGCGGAGTGAAGGGTCCCCTCTGGTGGTTCTGCCCTATATGAAGCACGGAGATCTTCG CAATTTCATTCGAAACGAGACTCATAACCCAACTGTGAAAGATCTTATAGGATTCGGTCTTCAAGTAGCC AAGGGCATGAAATATCTTGCCAGCAAAAAGTTTGTCCACAGAGACTTAGCTGCAAGAAACTGCATGTTGG ATGAAAAATTCACTGTCAAGGTTGCTGATTTCGGTCTTGCCAGAGACATGTACGACAAAGAGTATTATAG CGTCCACAACAAAACGGGTGCGAAACTACCGGTGAAGTGGATGGCTTTAGAGAGTCTGCAGACGCAAAAG TTCACCACCAAGTCAGACGTGTGGTCCTTCGGTGTGCTTCTCTGGGAGCTCATGACGAGAGGAGCCCCTC CTTATCCTGACGTGAACACATTTGATATCACTATATACCTGTTGCAAGGCAGAAGACTCTTGCAACCAGA GTACTGTCCAGACGCCTTGTATGAAGTGATGCTAAAATGCTGGCACCCCAAAGCAGAAATGCGCCCATCG TTTTCTGAACTGGTCTCCAGAATATCCTCAATCTTCTCCACTTTCATTGGCGAGCACTATGTCCATGTGA ACGCTACTTATGTGAATGTAAAATGTGTTGCTCCATATCCTTCTCTGTTGCCATCCCAAGACAACATTGA CGGCGAAGCGAACACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_031517
Insert Size	4149 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031517.1, NP_113705.1
RefSeq Size	4187 bp
RefSeq ORF	4149 bp
Locus ID	24553
UniProt ID	P97523
Gene Summary	hepatocyte growth factor receptor; functions as a heterodimeric tyrosine kinase [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.