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Tsc1 (NM_021854) Rat Untagged Clone
CAT＃: RN213270
Tsc1 (untagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug)
CNY 18,720.00
货期*
7周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213270 representing NM_021854
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCTAGCCAACATTGGGGAGCTACTCTCCATGCTGGACTCTTCCACACTGGGTGTGCGTGATG ACGTGACAACCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTAAACACGTTGGTGGA TTATTACCTGGAAACCAATTCTCAGCCGGTCTTGCACATCCTGACCACCCTGCAAGAGCCACATGATAAG CACCTCTTGGACAAAATGAATGAGTATGTAGGCAAAGCTGCCACCCGCTTATCCATCCTCTCGCTGCTGG GGCATGTTGTGAGGCTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTACT GAAATGTCTCAAGATGGACACTGATGTTGTGGTTCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTA CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTTTCATCAT GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTCTACCTGGTCCACCTCCATGCCAGTGTTTATGCCCT CTTCCATCGCCTTTATGGAATGTACCCTTGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGCATGAAG GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT AGAGTGTGCCAAAATCTCTCTGGACCCTACCGAAGCCTCATATGAAGATGGCGATGCTGTGTCTCACCAG CTCTCTGCCTGCTTCCCTCACCGTTCAGCTGATGTCACCACCAGCTCTTACGTGGACACACAGAATAGCT ATGGGGGCGCTACTTCCACCCCTTCCTCCACCTCTCGGCTGATGTTGTTCAGTACCCCCGGGCAGCTACC TCAGAGTTTGAGTTCACTATCAACACGGCCGTTACCTGAGCCGCTGCAAGCTAGTCTTTGGAGCCCATCT GCAGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCGGCTGATTTGTCACATCCATACA GTAAAGCCTTTGGTACTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCCCC AGCCCCGCCTTGTCCCCAAGACGACTGTGCGCACGGTCCAGCCTCCCAGGCCTCAGCCACACCCCCCAGG AAGGAAGAAAGAGCAGATTCTTCAAGGCCTTACCTACCCAGACAGCAGGATGTTCCGAGTGACCGCGGAT TAGAGGATCTACCTGGAAGCAAAGGTTCCGTCACTCTGAGGAATCTACCAGATTTCCTAGGTGACCTGGC TTCAGAGGAAGACAGTATAGAGAAAGATAAGGAAGAAGCTGCGATATCTAAAGAGCTTTCTGAGATCACC ACTGCAGAGGCGGATCCTGTAGCTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTG GTTCTCAGCGGAAGACTCACTCAGCAGCCTCTGGGACTCAGGGCTTCAGCGTGAACCCTGAGCCTTTGCA CTCCTCCCTGGACAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGC AGTGCTGATGCCAGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCA GCCCTTGCAAAATTCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCT CTTTGAGGTGGCCTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAAAAGACTGAAGAGCTGTTGAAGAAA GCGAAAGGAAACCCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGCTAG AGCAGGGAGCAGGGGCACACAGCAAGGAACTGAGCAGGTTGTCCTTGCCCAGCAAGTCTGTTGACTGGAC CCACTTTGGAGGCTCTCCCCCCTCAGATGAGATCCGGACCCTCCGAGACCAGTTGCTCTTACTGCACAAT CAGCTGCTGTACGAACGCTTTAAGCGGCAGCAGCATGCACTGAGGAACAGAAGGCTGCTGCGCAAGGTGA TCCGAGCTGCGGCCCTGGAGGAGCACAACGCAGCGATGAAAGATCAGTTGAAGTTACAAGAGAAGGACAT CCAGATGTGGAAGGTGAGTCTGCAGAAAGAACAAGCCCGATACAGTCAGCTTCAGCAACAACGTGACACC ATGGTGACCCAGCTGCATAGCCAAATCAGACAGCTGCAGCATGACCGAGAAGAATTCTACAACCAGAGTC AGGAGTTACAGACGAAGCTGGAGGACTGCAGAAGCATGATCGCGGAGCTTCGAGTGGAGCTGAAGAAGGC CAACAGCAAGGTGTGCCACACCGAGCTGCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAATAGTGAGTCA GTGCAGCAGCAGATGGAGTTCTTGAATAGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGG AGCAGCTGCAGAGCAAGCATCCTGACACCACCAAGGAAGTAGAAATGATGAAAACTGCTTATCGGAAAGA ACTAGAGAAAAACAGAAGCCACCTTCTCCAGCAGAACCAGAGGCTGGATGCCTCACAGAGGCGAGTTTTA GAACTGGAATCTCTTCTGGCCAAGAAAGACCACCTTCTCCTAGAGCAGAAGAAGTACCTTGAGGATGTCA AGAGCCAGGCGAGTGGACAGCTGCTGGCTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGT GTTGGAACTGGAGATCCTAGACTTGTATGGTAGATTGGAGAAAGATGGCCGCCTACAGAAACTAGAAGAG GACAGAGCAGAGGCAGCAGAGGCAGCTGAAGAGAGGCTTGACTGTTGTACTGATGGATGCTCAGATTCCT TGTTAGGACATAATGAAGAGGCTGCTGGTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCG GGCCAGCTGTGGAGGTAGAGTCACTGGAGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAA CCCCCGAACCAGAGGTTCAGCAGCCGGTGGGAGCCCACCATGGGTGAGCCCTCCAGCAGCATCCCCACCA CTGTTGGCTCACTTCCCAGTTCCAAAAGCTTCTTGGGCATGAAGACCCGGGAGCTGTTCCGTAATAAGAG TGAGAGCCAGTGTGATGAGGATGGCATGACCATGAGTAGCTTTTCTGAGACCCTGAAGACAGAACTGGGC AAAGACTCGGCGGGCATGGAAAACAAGACTCCCCCAAGTCTAGACGCCCCACACCCATCTTCCCCAAGCT CAGACAGTATGGGGCAGCTCCATATCATGGACTACAATGAGACTCATCACGAACACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_021854 |
| Insert Size | 3492 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021854.1, NP_068626.1 |
| RefSeq Size | 3492 bp |
| RefSeq ORF | 3492 bp |
| Locus ID | 60445 |
| UniProt ID | Q9Z136 |
| Gene Summary | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (By similarity). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (By similarity). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213270 | Tsc1 (Myc-DDK-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 18,720.00 |
|
| RR213270L3 | Lenti ORF clone of Tsc1 (Myc-DDK-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 20,240.00 |
|
| RR213270L4 | Lenti ORF clone of Tsc1 (mGFP-tagged ORF) - Rat tuberous sclerosis 1 (Tsc1), (10 ug) |
CNY 22,230.00 |
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