Oplah (NM_053904) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MGC105359
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213448 representing NM_053904 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCCCAGAAGGGCGCTTCCATTTCGCCATCGACCGCGGTGGCACCTTCACAGATGTCTTTGCCC AGTGCCCTGGAGGGCATGTGCGTGTCCTGAAGCTGCTCTCAGAGGACCCTGCCAACTATCCAGATGCACC CACAGAGGGCATCCGCCGAATTCTAGAGCAGGAGGAGGGTGTGCTGCTGCCTCGAGGCCGACCGCTAGAC ACCAGTCGCATTGCCAGCATCCGCATGGGTACCACGGTGGCCACCAATGCACTGTTGGAACGACAGGGAG AACGGGTGGCACTGCTGGTGACTCGGGGTTTCCGAGACCTGCTGCATATTGGCACTCAGGCCCGCCCGGA CCTCTTTGACTTGGCTGTGCCCATGCCAGAGGTTCTGTATGAGGAAGTGCTGGAGGTAGATGAGCGAGTG GTGCTGTATCGCGGAGAACCAGGTGCCGGCTCTCCTGTCAAAGGCCGCACAGGGGACCTGCTAGAGATAC AGCAGCCTGTGGACCTGGAAGCCCTGCGTGGGAAGCTGGAGGGGCTCTTGTCTCGGGGCATTCACAGTCT GGCAGTGGTGCTCATGCATTCGTACACGTGGGCCCAGCATGAGCAGCAGGTGGGCACGCTGGCCCGGGAG CTGGGCTTCACGCACGTCTCCTTGTCCTCGGAAGTCATGCCCATGGTACGAATTGTTCCTCGGGGCCATA CAGCCTGTGCTGACGCTTACCTTACTCCCACCATCCAGCGCTATGTGCAGGGCTTCCGCCGAGGCTTCCA GGGCCAGCTAAAGAATGTGCAAGTTCTCTTCATGCGCTCTGATGGTGGCCTCGCACCCATGGATGCTTTC AGTGGTTCCCGGGCTGTGCTCTCTGGCCCTGCTGGGGGTGTGGTTGGCTACTCAGCTACCACCTACCATC TGGAAGGCGGTCAGCCTGTCATTGGCTTTGACATGGGAGGCACATCCACAGACGTGAGCCGCTATGCTGG AGAATTTGAGCATGTCTTTGAGGCTAGCACAGCAGGCGTTACCCTTCAGGCACCCCAGTTGGACATCAAC ACAGTGGCAGCTGGCGGGGGTTCCCGCCTCTTCTTCAGATCTGGCCTCTTTGTGGTTGGTCCAGAGTCAG CAGGTGCCCACCCAGGTCCTGCCTGCTACCGTAAAGGGGGTCCTGTGACAGTGACAGATGCTAATCTGGT CCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCAGGAGAAGACCAGCCACTGTCTCCT GAGGCTTCCCGAAAGGCTCTAGAGGCTGTGGCCATGGAGGTCAACAGTTTCTTGACCAATGGACCGTGCC CAGCTTCCCAACTAAGTCTGGAAGAGGTGGCCATGGGGTTTGTGCGTGTTGCCAATGAAGCCATGTGCCG GCCTATCCGTGCCCTCACACAGGCACGAGGCCATGACCCCTCAGCCCATGTATTGGCTTGCTTTGGAGGA GCTGGTGGGCAACACGCTTGTGCCATTGCCCGGGCCCTGGGGATGGATACTGTGCACATTCACAGGCACA GCGGGCTGCTGTCAGCACTAGGACTGGCCTTGGCAGATGTGGTTCACGAAGCACAGGAGCCCTGTTCCCT GTCTTACACACCTGAAACCTTTGCACAACTGGACCAGAGACTGAGCCGCCTGGAGGAGCAGTGTGTGGAT GCCTTGCAGGTCCAGGGCTTCCCTAGGTCTCAGATCAGCACCGAGAGCTTCCTGCATCTTCGCTACCAAG GCACTGACTGCGCCCTAATGGTGTCTGCCCATCAGCATCCGGCCACAGCCTGCTCACCCCGAGCTGGTGA CTTTGGAGCCGCATTTGTGGAGAGGTACATGAGAGAGTTTGGCTTCATTATCCCCGAGCGGCCGGTGGTG GTAGATGATGTACGTGTGAGGGGAACTGGCCGTAGTGGACTTCAGCTGGAGGACACCCCCAAAATCCAGA CTGGACCTCCCCACGTGGAAAAGGTGACCCAGTGCTACTTTGAAGGGGGTTATCAGGAGACTCCCGTGTA CCTTTTAGGAGAACTAGGCTACGGGCACCAGCTCCAAGGGCCCTGCCTTATCATCGACAACAACAGCACC ATCCTTGTAGAACCGGGTTGCCAAGCAGAGGTGACTGATACAGGGGACATCCGCATTTCTGTGGGAGCTG AGGGTCCTAGTATGGCAGATACCAGGCTTGACCCCATCCAGCTGTCTATTTTCTCACACCGCTTCATGAG CATTGCTGAGCAGATGGGCCGCATCCTACAGCGCACAGCCATCTCTACCAACATCAAGGAACGCCTCGAC TTCTCCTGTGCCCTCTTTGGGCCAGATGGGGGCCTCGTCTCCAATGCTCCCCACATTCCTGTGCACCTGG GTGCCATGCAAGAGACTGTACAGTTCCAGATTCAGCACTTAGGAGCCGACCTCCATCCTGGTGATGTGTT GCTCAGCAACCATCCCAGCGCAGGGGGCAGCCATCTTCCTGACCTGACTGTCATTACACCGGTGTTTTGG CCAGGCCAGACGAGGCCTGTGTTCTACGTGGCTAGCCGAGGGCACCACGCAGACATTGGAGGAATCACAC CGGGCTCTATGCCGCCTCACTCCACCACGCTGCAACAGGAGGGTGCCGTTTTTCTGTCCTTCAAACTGGT CCAGGGAGGCGTCTTCCAGGAAGAGGCAGTGACAGAGGCCCTACGGGCACCAGGCAAGATCTCTGGCTGT AGTGGAACCAGGAACCTGCATGACAACCTGTCGGATCTTCGTGCCCAGGTGGCAGCTAACCAGAAAGGCA TCCAGCTGGTGGGAGAGCTGATCGGACAGTATGGCTTAGATGTGGTGCAGGCCTATATGGGCCATATTCA GGCGAATGCTGAGCTAGCAGTGAGAGACATGCTCCGGGCTTTTGGAACTTCCCGGCAGGCCAGGGGCCTG CCCCTGGAGGTGTCTGCAGAGGATCACATGGATGATGGCTCTCCCATCTGTCTGCGTGTTCAGATCAACC TGAGTCAGGGCAGTGCGGTATTTGACTTTACTGGTTCCGGGTCTGAGGTGTTTGGCAATCTCAATGCCCC GAGAGCCATAACACTGTCTGCTCTCATCTATTGCTTACGCTGTCTAGTGGGCCGTGACATCCCACTTAAC CAGGGTTGCCTGGCTCCTGTGCGTGTCATAATTCCCAAAGGCTCCATATTGGATCCATCCCCAGAGGCAG CAGTGGTCGGCGGCAACGTGCTCACATCTCAGCGAGTAGTGGATGTCATTCTGGGGGCTTTTGGGGCCTG TTCAGCCTCCCAGGGCTGCATGAACAATGTGACCCTGGGCAATGCCCGTATGGGCTACTATGAGACAGTG GCTGGTGGTGCCGGTGCGGGCCCTGGCTGGCATGGGCGCAGTGGTGTACACAGTCACATGACCAACACAC GCATTACGGATCCAGAGATTCTGGAGAGTCGGTATCCAGTTATCCTGCGCCGCTTTGAGCTGAGGCCAGG CTCCGGGGGCCGAGGTCGCTTCCGGGGAGGTGATGGCGTAGTCCGAGAGCTGGTCTTTCGGGAAGAGGCG CTGTTGTCTGTGCTCACCGAGCGCCGGGCCTTCCAGCCTTACGGCCTCCACGGGGGAGAGCCTGGTGCGC GTGGCTTAAACCTCCTGATCAGAAAAGATGGGCGCACAGTGAATTTGGGCGGCAAGACATCTGTGACCGT GTACCCCGGGGACGTGTTCTGCCTCCACACGCCTGGGGGTGGGGGCTACGGAGACCCGGAGGATCCAGCG CCACCACCAGGCTCGCCCCCGCTATTTCCAGCCTTCCCCGAGCGCGGCAGTGTATTCGAGTACCGCCGCG CCCAGGAAGCCGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053904
Insert Size	3867 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053904.1, NP_446356.1
RefSeq Size	4003 bp
RefSeq ORF	3867 bp
Locus ID	116684
UniProt ID	P97608
Gene Summary	catalyzes cleavage of 5-oxo-L-proline to form L-glutamate and is coupled to the hydrolysis of ATP to ADP and inorganic phosphate [RGD, Feb 2006]

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