Ncoa1 (NM_001108012) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	SRC-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213494 representing NM_001108012 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCCTTGGGGACAGTTCATCCGACCCTGCGAACCCAGACTCACATAAGAGAAAAGGATCGCCAT GTGACACACTGGCATCAAGTACTGAGAAGAGGCGCAGGGAGCAAGAAAATAAGTATTTAGAAGAACTAGC TGAATTACTGTCTGCCAACATCAGTGACATTGACAGCTTGAGTGTAAAACCAGATAAATGCAAGATCTTG AAGAAGACAGTAGATCAGATACAGCTAATGAAGAGAATGGAGCAAGAGAAATCCACAACTGATGATGACG TGCAGAAGTCCGACATCTCATCGAGCAGTCAAGGAGTGATAGAGAAGGAGTCGCTGGGCCCCCTTCTTCT GGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGAAGAATTGTATTTGTGTCAGAAAATGTG ACCAGCTACCTGGGTTACAATCAGGAAGAATTAATGAATACCAGCGTCTACAGCATACTGCATGTGGGAG ATCATGCAGAATTTGTCAAGAATCTACTACCAAAATCACTAGGCACTGAGAACCAAGAAGCTTGCCAGCG CTATGAAGTAATGCAGTGTTTCACGGTGTCACAGCCAAAATCAATCCAAGAAGATGGCGAAGGCAAAATC ATCTCTATTGACACTAGCTCCCTGAGAGCTGCTGGCAGGACTGGCTGGGAAGATTTAGTAAGGAAATGTA TCTATGCTTTTTTCCAACCTCAGGGCAGAGAGCCATCTTATGCCCGACAGCTGTTTCAAGAAGTGATGAC TCGTGGCACTGCCTCCAGCCCATCCTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACC AAGTGTAAACTTTGCTACCCTCAAAGTCCTGACATGCAGCCTTTCATCATGGGAATTCATATCATCGACA GGGAGCACAGTGGGCTTTCTCCTCAAGATGACACTAATTCTGGAATGTCAATTCCCCGAATAAACCCCTC AGTCAATCCTGGTATCTCTCCAGCCCATGGTGTGACCCGTTCATCCACGTTGCCACCATCCAACAACAAC ATGGTCTCTGCCAGAGCAAACCGCCAGCAGAGCACAGACCTTAACAGCGGCAGCAGTCACAGTAACTCTA GCAGCAGCCAAGGGAGCTTTGGATGCTCACCTGGAAATCAGATTGTAGCCAGCGTTGCCTTAAGCCAGGG ACAGGCAGGTTCCCAGAGCAGTAATCCGTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATT TCCCTCGCACAGTTCATGTCTCCGAGGAGACAAGCTAATTCTGGCTTGGCAACAAGGGCCAGGATGTCAA ACAATTCATTTCCTCCAAATATTCCAACATTAAGCTCCCCGGTTGGCATCACTAGCGGTGCCTGTAGTAA TAATAACCGATCCTATTCCAATATCCCAGTAACATCTTTACAAGGTATGAATGAAGGACCCAATAATTCT GTTGGCTTCTCTGCCGGCTCTCCAGTCCTTCGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAGTA TGCAACCAGCAAAAGCCGAGTCCAAAGACAACAAAGAGATTGCATCCATTTTAAATGAAATGATTCAGTC AGACAACAGTGCTAACGAAGGCAAGCCTCTGGACTCAGGACTTCTGCATAACAATGACAGACTCTCAGAT GGAGACAGTAAGTACTCTCAAACTAGTCACAAACTAGTACAGCTATTGACGACTACCGCAGAGCAGCAGT TACGGCATGCTGACATAGACACGAGCTGCAAAGACGTCCTGTCCTGCACTGGCACTTCCAGCTCCGCCTC CTCTAACCCCTCAGGAGGTACTTGTCCCTCTTCCCACAGCTCTCTGACTGAGCGGCATAAAATTCTGCAT CGGCTTTTACAGGAGGGCAGCCCCTCAGACATCACCACCTTGTCTGTGGAACCCGAAAAGAAGGACAGTG CGTCAGCTTCTACTGCTATGTCAGTGTCTGGACAAGCCCAGGGGAGTGCCAGCATAAAACTAGAACTGGA TGCTTCAAAGAAAAAAGAGTCAAAAGACCATCAGCTCCTACGCTACCTTTTAGACAAAGATGAGAAAGAT TTAAGATCAACTCCAAACCTGAGCCTGGATGACGTAAAGGTCAAAGTGGAGAAGAAAGAGCAGATGGATC CTTGTAACACAAACCCAGCCCCAGTGACCAAACCTGCTCCCGAGGAGGTTAAACTCGAGTCCCAGAGCCA GTTTTCAGCTGACCTTGACCAGTTTGATCAGTTATTGCCCACACTGGAGAAGGCAGCGCAGTTGCCGGGC TTGTGTGAGACAGACAGAATGGATGGTGCGGTCCCCGGTGTGAACATCAAGTCGGAGATCCTGCCCGCTT CACTTCAGCCCACCCCTGCCAGATCTGCTCCCAGGCTGAACAGATTGCCTGAACTAGAATTAGAAGCAAT CGATAACCAGTTTGGACAACCAGGAGCAGGGGATCAGATTCCATGGGCAAATAATACTGTGACGGCGATA AATCAGAGTAAACCAGAAGACCAATGTATTAGCTCACAGTTGGATGAGCTCCTCTGTCCACCAACAACAG TAGAAGGAAGAAATGATGAGAAGGCCCTTCTGGAACAACTGGTGTCTTTCCTCAGTGGCAAAGATGAGAC GGAGCTGGCCGAGCTAGACAGAGCGCTGGGGATTGACAAACTTGTACAGGGAGGTGGATTAGATGTATTA TCTGAGAGATTTCCACCACAGCAAGCAACACCACCTTTGATGATGGAAGACAGGCCCACCCTCTATCCCC AGCCATACTCGTCTCCTTCTCCCACCGCCGGTCTCTCTGGCCCTTTCCAAGGCATGGTCCGACAGAAGCC TTCACTGGGTACTATGCCGGTTCAAGTAACACCTCCTCGAGGAACCTTTTCACCCAACATGGGCATGCAA CCCCGGCAGACTCTAAATAGACCTCCAGCTGCACCCAACCAGCTTCGGCTGCAGCTGCAGCAGCGGCTAC AGGGGCAGCAGCAGTTGATGCACCAAAACCGGCAAGCTATCTTGAACCAGTTTGCAGCAAATGCTCCTGT TGGCATGAACATGAGGTCAGGCATGCAGCAGCAGATAACACCTCAGCCACCCCTGAATGCTCAGATGTTG GCACAGCGCCAACGGGAGCTGTACAGTCAGCAGCATCGACAGAGGCAAATTATCCAGCAGCAAAGAGCCA TGCTCATGAGGCACCAAAGCTTTGGGAACAACATCCCTCCTTCATCCGGACTCCCAGTTCAAATGGGGAA CCCCCGTCTTCCTCAGGGTGCTCCACAGCAGTTCCCCTACCCACCAAACTATGGTACAAATCCAGGAACC CCACCTGCTTCTACCAGCCCTTTCTCTCAACTGGCAGCAAACCCTGAGGCATCCTTGGCCACCCGCAGCA GCATGGTGAACAGAGGCATGGCAGGGAACATGGGAGGACAGTTTGGTACTGGCATCAGTCCGCAGATGCA GCAGAATGTCTTCCAGTACCCAGGGTCAGGACTGGTTCCCCAAGCTGAGGCCAACTTTGCCCCATCTCTA AGCCCTGGGAGCTCCATGGTGCCGATGCCAATCCCTCCTCAAAGCTCTCTGCTCCAACAAACTCCACCCA CTTCTGGGTACCAGTCACCGGACATGAAGGCCTGGCAGCAAGGAACAATGGGAAACAACAATGTGTTCAG TCAAGCCGTCCAGAGCCAGCCTGCCCCTGCACAGCCAGGAGTGTACAACAACATGAGCATCACCGTGTCC ATGGCAGGTGGAAACGCAAACGTTCAGAACATGAATCCAATGATGGGCCAGATGCAAATGAGCTCTCTGC AGATGCCAGGGATGAACACTGTGTGCTCTGAGCAGATGAATGATCCAGCACTGAGACACACAGGCCTCTA CTGCAACCAGCTCTCATCCACTGACCTTCTCAAAACCGAAGCAGATGGAAACCAGGACAAGAAGACAGAA GAGTTCTTCTCTGTGCAGGTGCAGCAGCTTCAGGTGTTTGCTGACGTCCAGTGTACAGTGAACCTGAAAG GAGCCAGCAAACCGACTCTAGTGCTAGCAGTTCTGCTACAAGAAAAGGCCATAAGAACCAATGCAGATGA GGAGCATGGACTCAAGACTTCAAGGAAGAAGCCATTTCCCCAGGTCCTTCCTTCTGCATCTCACCGCCCC TGGTACAAACAACTCCATAAGAACAGCATCTACTCAGAAACTACAGGACTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108012
Insert Size	4254 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108012.1, NP_001101482.1
RefSeq Size	5290 bp
RefSeq ORF	4254 bp
Locus ID	313929

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