Ncoa1 (NM_001108012) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SRC-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213494 representing NM_001108012 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCCTTGGGGACAGTTCATCCGACCCTGCGAACCCAGACTCACATAAGAGAAAAGGATCGCCAT GTGACACACTGGCATCAAGTACTGAGAAGAGGCGCAGGGAGCAAGAAAATAAGTATTTAGAAGAACTAGC TGAATTACTGTCTGCCAACATCAGTGACATTGACAGCTTGAGTGTAAAACCAGATAAATGCAAGATCTTG AAGAAGACAGTAGATCAGATACAGCTAATGAAGAGAATGGAGCAAGAGAAATCCACAACTGATGATGACG TGCAGAAGTCCGACATCTCATCGAGCAGTCAAGGAGTGATAGAGAAGGAGTCGCTGGGCCCCCTTCTTCT GGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGAAGAATTGTATTTGTGTCAGAAAATGTG ACCAGCTACCTGGGTTACAATCAGGAAGAATTAATGAATACCAGCGTCTACAGCATACTGCATGTGGGAG ATCATGCAGAATTTGTCAAGAATCTACTACCAAAATCACTAGGCACTGAGAACCAAGAAGCTTGCCAGCG CTATGAAGTAATGCAGTGTTTCACGGTGTCACAGCCAAAATCAATCCAAGAAGATGGCGAAGGCAAAATC ATCTCTATTGACACTAGCTCCCTGAGAGCTGCTGGCAGGACTGGCTGGGAAGATTTAGTAAGGAAATGTA TCTATGCTTTTTTCCAACCTCAGGGCAGAGAGCCATCTTATGCCCGACAGCTGTTTCAAGAAGTGATGAC TCGTGGCACTGCCTCCAGCCCATCCTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACC AAGTGTAAACTTTGCTACCCTCAAAGTCCTGACATGCAGCCTTTCATCATGGGAATTCATATCATCGACA GGGAGCACAGTGGGCTTTCTCCTCAAGATGACACTAATTCTGGAATGTCAATTCCCCGAATAAACCCCTC AGTCAATCCTGGTATCTCTCCAGCCCATGGTGTGACCCGTTCATCCACGTTGCCACCATCCAACAACAAC ATGGTCTCTGCCAGAGCAAACCGCCAGCAGAGCACAGACCTTAACAGCGGCAGCAGTCACAGTAACTCTA GCAGCAGCCAAGGGAGCTTTGGATGCTCACCTGGAAATCAGATTGTAGCCAGCGTTGCCTTAAGCCAGGG ACAGGCAGGTTCCCAGAGCAGTAATCCGTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATT TCCCTCGCACAGTTCATGTCTCCGAGGAGACAAGCTAATTCTGGCTTGGCAACAAGGGCCAGGATGTCAA ACAATTCATTTCCTCCAAATATTCCAACATTAAGCTCCCCGGTTGGCATCACTAGCGGTGCCTGTAGTAA TAATAACCGATCCTATTCCAATATCCCAGTAACATCTTTACAAGGTATGAATGAAGGACCCAATAATTCT GTTGGCTTCTCTGCCGGCTCTCCAGTCCTTCGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAGTA TGCAACCAGCAAAAGCCGAGTCCAAAGACAACAAAGAGATTGCATCCATTTTAAATGAAATGATTCAGTC AGACAACAGTGCTAACGAAGGCAAGCCTCTGGACTCAGGACTTCTGCATAACAATGACAGACTCTCAGAT GGAGACAGTAAGTACTCTCAAACTAGTCACAAACTAGTACAGCTATTGACGACTACCGCAGAGCAGCAGT TACGGCATGCTGACATAGACACGAGCTGCAAAGACGTCCTGTCCTGCACTGGCACTTCCAGCTCCGCCTC CTCTAACCCCTCAGGAGGTACTTGTCCCTCTTCCCACAGCTCTCTGACTGAGCGGCATAAAATTCTGCAT CGGCTTTTACAGGAGGGCAGCCCCTCAGACATCACCACCTTGTCTGTGGAACCCGAAAAGAAGGACAGTG CGTCAGCTTCTACTGCTATGTCAGTGTCTGGACAAGCCCAGGGGAGTGCCAGCATAAAACTAGAACTGGA TGCTTCAAAGAAAAAAGAGTCAAAAGACCATCAGCTCCTACGCTACCTTTTAGACAAAGATGAGAAAGAT TTAAGATCAACTCCAAACCTGAGCCTGGATGACGTAAAGGTCAAAGTGGAGAAGAAAGAGCAGATGGATC CTTGTAACACAAACCCAGCCCCAGTGACCAAACCTGCTCCCGAGGAGGTTAAACTCGAGTCCCAGAGCCA GTTTTCAGCTGACCTTGACCAGTTTGATCAGTTATTGCCCACACTGGAGAAGGCAGCGCAGTTGCCGGGC TTGTGTGAGACAGACAGAATGGATGGTGCGGTCCCCGGTGTGAACATCAAGTCGGAGATCCTGCCCGCTT CACTTCAGCCCACCCCTGCCAGATCTGCTCCCAGGCTGAACAGATTGCCTGAACTAGAATTAGAAGCAAT CGATAACCAGTTTGGACAACCAGGAGCAGGGGATCAGATTCCATGGGCAAATAATACTGTGACGGCGATA AATCAGAGTAAACCAGAAGACCAATGTATTAGCTCACAGTTGGATGAGCTCCTCTGTCCACCAACAACAG TAGAAGGAAGAAATGATGAGAAGGCCCTTCTGGAACAACTGGTGTCTTTCCTCAGTGGCAAAGATGAGAC GGAGCTGGCCGAGCTAGACAGAGCGCTGGGGATTGACAAACTTGTACAGGGAGGTGGATTAGATGTATTA TCTGAGAGATTTCCACCACAGCAAGCAACACCACCTTTGATGATGGAAGACAGGCCCACCCTCTATCCCC AGCCATACTCGTCTCCTTCTCCCACCGCCGGTCTCTCTGGCCCTTTCCAAGGCATGGTCCGACAGAAGCC TTCACTGGGTACTATGCCGGTTCAAGTAACACCTCCTCGAGGAACCTTTTCACCCAACATGGGCATGCAA CCCCGGCAGACTCTAAATAGACCTCCAGCTGCACCCAACCAGCTTCGGCTGCAGCTGCAGCAGCGGCTAC AGGGGCAGCAGCAGTTGATGCACCAAAACCGGCAAGCTATCTTGAACCAGTTTGCAGCAAATGCTCCTGT TGGCATGAACATGAGGTCAGGCATGCAGCAGCAGATAACACCTCAGCCACCCCTGAATGCTCAGATGTTG GCACAGCGCCAACGGGAGCTGTACAGTCAGCAGCATCGACAGAGGCAAATTATCCAGCAGCAAAGAGCCA TGCTCATGAGGCACCAAAGCTTTGGGAACAACATCCCTCCTTCATCCGGACTCCCAGTTCAAATGGGGAA CCCCCGTCTTCCTCAGGGTGCTCCACAGCAGTTCCCCTACCCACCAAACTATGGTACAAATCCAGGAACC CCACCTGCTTCTACCAGCCCTTTCTCTCAACTGGCAGCAAACCCTGAGGCATCCTTGGCCACCCGCAGCA GCATGGTGAACAGAGGCATGGCAGGGAACATGGGAGGACAGTTTGGTACTGGCATCAGTCCGCAGATGCA GCAGAATGTCTTCCAGTACCCAGGGTCAGGACTGGTTCCCCAAGCTGAGGCCAACTTTGCCCCATCTCTA AGCCCTGGGAGCTCCATGGTGCCGATGCCAATCCCTCCTCAAAGCTCTCTGCTCCAACAAACTCCACCCA CTTCTGGGTACCAGTCACCGGACATGAAGGCCTGGCAGCAAGGAACAATGGGAAACAACAATGTGTTCAG TCAAGCCGTCCAGAGCCAGCCTGCCCCTGCACAGCCAGGAGTGTACAACAACATGAGCATCACCGTGTCC ATGGCAGGTGGAAACGCAAACGTTCAGAACATGAATCCAATGATGGGCCAGATGCAAATGAGCTCTCTGC AGATGCCAGGGATGAACACTGTGTGCTCTGAGCAGATGAATGATCCAGCACTGAGACACACAGGCCTCTA CTGCAACCAGCTCTCATCCACTGACCTTCTCAAAACCGAAGCAGATGGAAACCAGGACAAGAAGACAGAA GAGTTCTTCTCTGTGCAGGTGCAGCAGCTTCAGGTGTTTGCTGACGTCCAGTGTACAGTGAACCTGAAAG GAGCCAGCAAACCGACTCTAGTGCTAGCAGTTCTGCTACAAGAAAAGGCCATAAGAACCAATGCAGATGA GGAGCATGGACTCAAGACTTCAAGGAAGAAGCCATTTCCCCAGGTCCTTCCTTCTGCATCTCACCGCCCC TGGTACAAACAACTCCATAAGAACAGCATCTACTCAGAAACTACAGGACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213494 representing NM_001108012 Red=Cloning site Green=Tags(s) MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKIL KKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENV TSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLGTENQEACQRYEVMQCFTVSQPKSIQEDGEGKI ISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHT KCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRINPSVNPGISPAHGVTRSSTLPPSNNN MVSARANRQQSTDLNSGSSHSNSSSSQGSFGCSPGNQIVASVALSQGQAGSQSSNPSLNLNNSPMEGTGI SLAQFMSPRRQANSGLATRARMSNNSFPPNIPTLSSPVGITSGACSNNNRSYSNIPVTSLQGMNEGPNNS VGFSAGSPVLRQMSSQNSPSRLSMQPAKAESKDNKEIASILNEMIQSDNSANEGKPLDSGLLHNNDRLSD GDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSSSASSNPSGGTCPSSHSSLTERHKILH RLLQEGSPSDITTLSVEPEKKDSASASTAMSVSGQAQGSASIKLELDASKKKESKDHQLLRYLLDKDEKD LRSTPNLSLDDVKVKVEKKEQMDPCNTNPAPVTKPAPEEVKLESQSQFSADLDQFDQLLPTLEKAAQLPG LCETDRMDGAVPGVNIKSEILPASLQPTPARSAPRLNRLPELELEAIDNQFGQPGAGDQIPWANNTVTAI NQSKPEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVL SERFPPQQATPPLMMEDRPTLYPQPYSSPSPTAGLSGPFQGMVRQKPSLGTMPVQVTPPRGTFSPNMGMQ PRQTLNRPPAAPNQLRLQLQQRLQGQQQLMHQNRQAILNQFAANAPVGMNMRSGMQQQITPQPPLNAQML AQRQRELYSQQHRQRQIIQQQRAMLMRHQSFGNNIPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGT PPASTSPFSQLAANPEASLATRSSMVNRGMAGNMGGQFGTGISPQMQQNVFQYPGSGLVPQAEANFAPSL SPGSSMVPMPIPPQSSLLQQTPPTSGYQSPDMKAWQQGTMGNNNVFSQAVQSQPAPAQPGVYNNMSITVS MAGGNANVQNMNPMMGQMQMSSLQMPGMNTVCSEQMNDPALRHTGLYCNQLSSTDLLKTEADGNQDKKTE EFFSVQVQQLQVFADVQCTVNLKGASKPTLVLAVLLQEKAIRTNADEEHGLKTSRKKPFPQVLPSASHRP WYKQLHKNSIYSETTGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108012
ORF Size	4251 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108012.1, NP_001101482.1
RefSeq Size	5290 bp
RefSeq ORF	4254 bp
Locus ID	313929
MW	153.8 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.