Smc1a (NM_031683) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	SB1.8; SMC-1A; Smc1l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214207 representing NM_031683 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTCCTGAAACTGATTGAGATTGAGAACTTTAAATCGTACAAGGGTCGACAAATTATCGGACCAT TTCAGAGGTTCACCGCCATCATTGGACCCAATGGCTCTGGTAAGTCAAATCTCATGGATGCCATCAGCTT TGTGCTTGGTGAAAAAACCAGCAACCTGAGGGTAAAGACCCTGAGGGACCTGATACACGGAGCTCCTGTG GGCAAGCCGGCTGCCAACCGAGCCTTTGTCAGCATGGTCTACTCCGAGGAGGGTGCTGAGGACCGCACCT TTGCCCGAGTCATTGTAGGGGGCTCTTCTGAGTACAAGATCAATAACAAAGTTGTCCAGCTACATGAATA CAGTGAAGAGTTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTCTTCCAGGGTGCAGTG GAATCTATTGCCATGAAGAACCCCAAGGAGAGGACAGCTTTATTTGAAGAAATTAGTCGCTCTGGGGAAC TTGCACAGGAGTATGACAAGCGAAAGAAAGAAATGGTGAAGGCTGAAGAAGACACACAGTTTAATTATCA TCGCAAGAAAAATATTGCAGCTGAACGCAAGGAGGCCAAACAAGAAAAGGAAGAGGCTGACCGCTACCAG GCCCTGAAGGATGAGGTAGTACGCGCACAGGTACAATTGCAGCTTTTTAAACTCTACCACAACGAAGTGG AGATTGAGAAGCTCAACAAGGAACTGGCCTCGAAGAACAAGGAAATTGAGAAGGACAAAAAGCGGATGGA CAAGGTAGAGGATGAACTGAAGGAGAAGAAGAAAGAGCTGGGCAAAATGATGCGGGAACAGCAGCAGATT GAGAAGGAGATCAAGGAAAAGGACTCAGAATTGAATCAGAAGAGGCCTCAGTACATCAAAGCAAAAGAGA ATACATCCCACAAAATCAAGAAGCTTGAAGCAGCCAAGAAATCTCTGCAGAATCGTCAGAAACATTATAA GAAACGTAAAGGTGACATGGATGAGCTGGAGAAAGAGATGCTGTCAGTGGAGAAGGCCCGACAGGAGTTT GAAGAGCGGATGGAGGAAGAGAGTCAGAGTCAAGGCAGAGACTTGACCCTGGAGGAGAACCAGGTAAAGA AATACCACCGATTAAAAGAGGAAGCTAGCAAGAGAGCAGCCACTTTGGCTCAGGAGCTGGAGAAGTTTAA TCGAGACCAGAAAGCTGACCAGGACCGTCTAGATCTAGAAGAGCGGAAGAAAGTAGAGACAGAGGCCAAG ATCAAGCAAAAACTGCGGGAGATTGAAGAGAATCAGAAACGGATTGAGAAGCTAGAGGAATATATTACCA CTAGCAAGCAGTCTCTAGAGGAACAGAAGAAGCTAGAGGGAGAATTGACAGAAGAAGTAGAGATGGCCAA GCGTCGTATTGATGAGATCAATAAGGAGCTGAACCAGGTGATGGAGCAATTGGGGGATGCGCGCATTGAC CGGCAGGAGAGCAGCCGCCAGCAGAGGAAGGCAGAAATAATGGAAAGCATCAAGCGTCTCTACCCTGGTT CTGTGTATGGCCGCCTCATTGACCTCTGCCAGCCTACACAAAAGAAGTATCAGATTGCTGTGACCAAGGT TTTAGGAAAGAACATGGATGCTATTATTGTAGACTCCGAGAAGACAGGTCGGGATTGTATTCAGTATATC AAGGAGCAACGTGGAGAGCCTGAGACCTTCTTGCCTCTTGACTACCTGGAGGTGAAACCTACTGATGAGA AACTCCGAGAACTGAAGGGGGCCAAGCTAGTGATTGATGTGATTCGCTATGAGCCACCTCACATCAAAAA AGCTCTGCAGTATGCTTGTGGCAATGCCCTTGTTTGTGACAATGTGGAGGATGCGCGACGAATTGCCTTT GGAGGTCACCAACGCCACAAGACAGTGGCATTGGATGGCACCCTGTTCCAGAAGTCAGGAGTTATCTCTG GTGGAGCCAGTGATCTCAAGGCAAAGGCACGACGCTGGGATGAGAAGGCAGTAGACAAACTAAAAGAAAA AAAAGAACGTTTGACGGAGGAGCTAAAAGAGCAGATGAAGGCAAAACGAAAAGAGGCAGAGCTGCGTCAG GTGCAGTCTCAGGCCCATGGGCTACAAATGCGGCTGAAGTACTCACAGAGTGACCTAGAACAGACTAAGA CAAGGCATCTTGCCCTGAATCTCCAGGAAAAATCTAAGCTGGAGAGTGAACTAGCCAACTTCGGGCCTCG AATTAATGATATCAAGAGAATCATACAGAGCCGAGAGAGGGAAATGAAAGACTTGAAGGAGAAGATGAAC CAGGTGGAAGATGAGGTGTTTGAAGAGTTTTGTCGGGAGATTGGTGTGCGTAACATCCGCGAATTTGAGG AAGAAAAGGTGAAGCGGCAGAATGAAATTGCCAAGAAACGCTTGGAATTTGAGAATCAGAAGACTCGCTT GGGCATCCAGTTGGATTTTGAAAAGAATCAGTTGAAGGAGGACCAGGATAAAGTACACATGTGGGAGCAG ACAGTAAAAAAGGATGAAAATGAGATAGAAAAGCTTAAGAAGGAGGAGCAAAGACACATGAAGATAATAG ATGAAACCATGGCTCAGCTCCAAGACCTTAAGAATCAGCACCTGGCCAAAAAGTCAGAAGTGAATGACAA GAATCATGAGATGGAAGAAATTCGCAAGAAGCTTGGGGGTGCCAATAAGGAAATGACTCACTTACAGAAG GAAGTGACAGCCATTGAGACCAAGCTTGAACAGAAGCGCAGTGACCGCCACAACCTGCTACAGGCCTGTA AGATGCAGGACATCAAATTGCCACTGTCAAAGGGCACCATGGATGATATTAGTCAGGAAGAAGGTGGCTC CCAGGGAGAAGAGTCAGTGAGTGGTTCCCAGAGAACATCTAGTATCTATGCACGAGAGGCCCTCATTGAG ATTGACTACGGTGACCTGTGTGAAGATCTGAAGGATGCACAGGCGGAAGAAGAAATCAAGCAAGAGATGA ACACACTCCAGCAAAAGCTGAATGAGCAGCAGAGTGTGCTTCAGAGGATTGCTGCCCCTAACATGAAGGC CATGGAAAAGCTGGAAAGTGTCCGAGACAAGTTCCAGGAGACCTCAGATGAATTTGAGGCAGCACGAAAG CGAGCAAAGAAAGCCAAGCAGGCATTTGAACAGATAAAGAAGGAGCGCTTTGACCGATTCAATGCTTGTT TTGAATCTGTGGCTACCAATATTGATGAGATCTACAAGGCTCTGTCCCGTAACAGCAGTGCCCAGGCATT CTTGGGCCCCGAGAATCCTGAAGAGCCCTATTTGGATGGTATCAACTACAACTGTGTAGCTCCTGGCAAA CGTTTCCGGCCTATGGACAATTTGTCAGGCGGGGAGAAGACAGTTGCCGCACTGGCCTTGCTTTTTGCCA TTCACAGCTACAAGCCAGCCCCCTTCTTCGTCCTGGATGAGATTGATGCTGCCTTGGATAACACCAACAT TGGCAAGGTGGCAAATTACATCAAGGAGCAGTCAACTTGCAACTTCCAGGCCATTGTTATCTCTCTCAAG GAGGAATTCTACACCAAGGCTGAGAGCCTCATTGGAGTCTATCCTGAGCAAGGAGACTGTGTGATCAGCA AAGTCCTGACCTTCGACCTCACCAAGTATCCAGATGCCAACCCTAACCCCAATGAACAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031683
Insert Size	3702 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031683.1, NP_113871.1
RefSeq Size	3933 bp
RefSeq ORF	3702 bp
Locus ID	63996
UniProt ID	Q9Z1M9
Gene Summary	structural maintenance of chromosomes (SMC) protein, one of 2 component proteins forming the cohesin multiprotein complex required for sister chromatid cohesion; thought to be a component of functional kinetochores [RGD, Feb 2006]

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