Slc12a2 (NM_031798) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Bsc2; Nkcc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214390 representing NM_031798 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGA CTGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTACTCTCCGGTCGAGAGGATGCTACCGCTGGGAGCCA GGCCGGCGGCGGGGTGCGCGGAGAGGGAACCCCGGCCGCTGGCGATGGGCTGGGCAAACCTCTGGGGCCC ACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCTGCTGCAG CTGCAGCGGCGGCTGCGGCGGCTGCCGGGGCAGCGGGCAAGGAGACCCCCGCGGCTGGGAAAGCCGGCAC TGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACCCGGCT GCCTCCTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGAGTCGGTGGCGACGGGCCCAACGTGAGCT CCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGTGGTGGCAGTGGACACCA CCAGCAGTACTACTATGACACCCACACCAACACTTACTACCTGCGCACCTTCGGCCACAACACCATGGAC GCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCCAGCC TGGGAGAGTTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGTTTTGCGAATGGAGAAGAAAGTAC TCCAACCAGAGATGCTGTGGTCACATACACTGCCGAAAGTAAAGGAGTTGTAAAGTTTGGCTGGATCAAG GGTGTTTTAGTACGATGTATGTTGAATATTTGGGGTGTGATGCTCTTTATTCGATTGTCATGGATTGTGG GTCAGGCTGGAATAGGTCTCTCCGTTCTTGTAATAGCAATGGCCACTGTTGTGACAACCATCACAGGATT GTCTACTTCAGCAATAGCTACCAATGGATTCGTGAGAGGAGGAGGAGCATACTATTTAATCTCTAGAAGT CTAGGGCCAGAATTTGGCGGTGCGATTGGCTTGATCTTCGCTTTTCCCAATGCAGTGGCAGTTGCTATGT ATGTTGTTGGATTCGCAGAGACTGTAGTGGAGCTGCTTAAGGAACATTCCATACTTATGATAGATGAAAT CAATGACATCCGAATTATTGGAGCCATTACAGTGGTGGTTCTTCTGGGCATCTCAGTGGCCGGAATGGAG TGGGAAGCAAAGGCTCAGATCGTCCTTCTGGTGATTCTTCTTCTGGCCATCGCTGACTTCGTCATAGGAA CATTTATCTCACTGGACAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATTTAGTGAGAA CTTTGGACCGGATTTTCGAGAAGAAGAGACTTTCTTTTCTGTATTCGCCATCTTTTTTCCTGCTGCAACT GGTATTCTAGCTGGGGCGAACATCTCGGGTGACCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACGC TCTTAGCCATTTTGATTACGACAGTGGTTTACATAGGAATCGCAGTGTCTGTAGGTTCTTGTGTTGTCCG GGATGCCACTGGGAACGTTAATGACACCATTACAACAGAGCTAACTAACTGTACTTCTGCAGCCTGCAAA TTAAACTTTGACTTTTCCTACTGTGAAAGCAACACCTGTTCTTATGGGCTAATGAACAACTTTCAGGTGA TGAGCATGGTGTCAGGATTTGCACCCCTGATCTCTGCGGGTATCTTTTCAGCCACACTCTCATCAGCACT GGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCAGCTTTCCAG ATGTTTGCGAAAGGTTATGGAAAAAATAATGAACCTCTTCGTGGTTACATCTTAACATTCTTAATTGCAC TTGGATTCATCTTAATTGCTGAATTGAATGTCATTGCTCCAATTATCTCTAATTTCTTTCTCGCATCATA CGCCTTAATCAACTTTTCTGTATTCCATGCGTCACTTGCAAAGTCTCCAGGGTGGCGTCCTGCGTTCAAG TACTACAACATGTGGATCTCCCTTACTGGAGCGATTCTCTGCTGCATAGTGATGTTCGTCATTAACTGGT GGGCTGCACTGCTCACATATGTGATAGTTCTTGGACTGTACATTTATGTCACTTACAAAAAGCCAGATGT GAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGCGCACTGCAGCATTCAATCCGTCTTTCCGGG GTGGAGGACCATGTGAAAAACTTCAGGCCACAGTGTCTTGTAATGACAGGTTCTCCAAACTCACGGCCTG CTTTACTGCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGTGCATATGGG TCCCCGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAAATATCAGCGATGGCTCATTAAAAAC AAAATGAAGGCTTTCTACGCTCCGGTTCACGCAGATGACTTGCGGGAAGGTGCACAGTACCTGATGCAGG CTGCTGGACTTGGTCGTATGAAACCAAACACACTTGTCCTCGGATTTAAGAAAGATTGGTTACAAGCAGA TATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAGTATGGAGTTGTGGTC ATTCGCCTAAAGGAAGGACTGGATATATCACACCTCCAAGGACAAGAAGAATTATTGTCATCACAAGAAA AGTCACCTGGTACCAAGGATGTGGTAGTAAATGTGGACTACAGTAAGAAGTCAGATCAAGATGCTTTCAA AGCATCTGGTGAAAAACCTATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTTCAACTCAGCCACTG TTGAAAAAAGAATCCAAGGGCCCCGTGGCACCTTTAAATGTAGCTGACCAAAAGCTTCTTGAAGCTAGCA CACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGGAGGTTTGAC CTTGTTGATACCCTATCTTCTGACAACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTATTCATTGGT GGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTACTCAGTAAATTCCGAATAGACT TCTCCGATATCATGGTCCTAGGAGACATCAACACCAAACCAAAAAAGGAAAACATCGTCGCTTTTGATGA CATGATTGAGCCATACAGACTTCACGAGGATGATAAAGAGCAGGATATTGCAGACAAAATGAAAGAGGAT GAGCCCTGGCGCATCACAGACAACGAGCTGGAGCTCTACAAGACCAAGACATATCGGCAGATCAGGTTAA ATGAATTATTAAAGGAGCATTCAAGCACAGCTAATATTATTGTCATGAGTCTTCCAGTTGCCCGGAAGGG TGCTGTGTCCAGCGCGCTGTACATGGCTTGGTTAGAAGCTCTGTCTAAGGACCTGCCACCAATCCTGCTG GTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031798
Insert Size	3612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031798.1, NP_113986.1
RefSeq Size	6402 bp
RefSeq ORF	3612 bp
Locus ID	83629
Gene Summary	Na/K/2Cl cotransporter involved in maintainence of intracellular Cl(-) levels [RGD, Feb 2006]

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