Slc12a2 (NM_031798) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bsc2; Nkcc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214390 representing NM_031798 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGA CTGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTACTCTCCGGTCGAGAGGATGCTACCGCTGGGAGCCA GGCCGGCGGCGGGGTGCGCGGAGAGGGAACCCCGGCCGCTGGCGATGGGCTGGGCAAACCTCTGGGGCCC ACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCTGCTGCAG CTGCAGCGGCGGCTGCGGCGGCTGCCGGGGCAGCGGGCAAGGAGACCCCCGCGGCTGGGAAAGCCGGCAC TGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACCCGGCT GCCTCCTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGAGTCGGTGGCGACGGGCCCAACGTGAGCT CCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGTGGTGGCAGTGGACACCA CCAGCAGTACTACTATGACACCCACACCAACACTTACTACCTGCGCACCTTCGGCCACAACACCATGGAC GCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCCAGCC TGGGAGAGTTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGTTTTGCGAATGGAGAAGAAAGTAC TCCAACCAGAGATGCTGTGGTCACATACACTGCCGAAAGTAAAGGAGTTGTAAAGTTTGGCTGGATCAAG GGTGTTTTAGTACGATGTATGTTGAATATTTGGGGTGTGATGCTCTTTATTCGATTGTCATGGATTGTGG GTCAGGCTGGAATAGGTCTCTCCGTTCTTGTAATAGCAATGGCCACTGTTGTGACAACCATCACAGGATT GTCTACTTCAGCAATAGCTACCAATGGATTCGTGAGAGGAGGAGGAGCATACTATTTAATCTCTAGAAGT CTAGGGCCAGAATTTGGCGGTGCGATTGGCTTGATCTTCGCTTTTCCCAATGCAGTGGCAGTTGCTATGT ATGTTGTTGGATTCGCAGAGACTGTAGTGGAGCTGCTTAAGGAACATTCCATACTTATGATAGATGAAAT CAATGACATCCGAATTATTGGAGCCATTACAGTGGTGGTTCTTCTGGGCATCTCAGTGGCCGGAATGGAG TGGGAAGCAAAGGCTCAGATCGTCCTTCTGGTGATTCTTCTTCTGGCCATCGCTGACTTCGTCATAGGAA CATTTATCTCACTGGACAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATTTAGTGAGAA CTTTGGACCGGATTTTCGAGAAGAAGAGACTTTCTTTTCTGTATTCGCCATCTTTTTTCCTGCTGCAACT GGTATTCTAGCTGGGGCGAACATCTCGGGTGACCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACGC TCTTAGCCATTTTGATTACGACAGTGGTTTACATAGGAATCGCAGTGTCTGTAGGTTCTTGTGTTGTCCG GGATGCCACTGGGAACGTTAATGACACCATTACAACAGAGCTAACTAACTGTACTTCTGCAGCCTGCAAA TTAAACTTTGACTTTTCCTACTGTGAAAGCAACACCTGTTCTTATGGGCTAATGAACAACTTTCAGGTGA TGAGCATGGTGTCAGGATTTGCACCCCTGATCTCTGCGGGTATCTTTTCAGCCACACTCTCATCAGCACT GGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCAGCTTTCCAG ATGTTTGCGAAAGGTTATGGAAAAAATAATGAACCTCTTCGTGGTTACATCTTAACATTCTTAATTGCAC TTGGATTCATCTTAATTGCTGAATTGAATGTCATTGCTCCAATTATCTCTAATTTCTTTCTCGCATCATA CGCCTTAATCAACTTTTCTGTATTCCATGCGTCACTTGCAAAGTCTCCAGGGTGGCGTCCTGCGTTCAAG TACTACAACATGTGGATCTCCCTTACTGGAGCGATTCTCTGCTGCATAGTGATGTTCGTCATTAACTGGT GGGCTGCACTGCTCACATATGTGATAGTTCTTGGACTGTACATTTATGTCACTTACAAAAAGCCAGATGT GAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGCGCACTGCAGCATTCAATCCGTCTTTCCGGG GTGGAGGACCATGTGAAAAACTTCAGGCCACAGTGTCTTGTAATGACAGGTTCTCCAAACTCACGGCCTG CTTTACTGCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGTGCATATGGG TCCCCGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAAATATCAGCGATGGCTCATTAAAAAC AAAATGAAGGCTTTCTACGCTCCGGTTCACGCAGATGACTTGCGGGAAGGTGCACAGTACCTGATGCAGG CTGCTGGACTTGGTCGTATGAAACCAAACACACTTGTCCTCGGATTTAAGAAAGATTGGTTACAAGCAGA TATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAGTATGGAGTTGTGGTC ATTCGCCTAAAGGAAGGACTGGATATATCACACCTCCAAGGACAAGAAGAATTATTGTCATCACAAGAAA AGTCACCTGGTACCAAGGATGTGGTAGTAAATGTGGACTACAGTAAGAAGTCAGATCAAGATGCTTTCAA AGCATCTGGTGAAAAACCTATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTTCAACTCAGCCACTG TTGAAAAAAGAATCCAAGGGCCCCGTGGCACCTTTAAATGTAGCTGACCAAAAGCTTCTTGAAGCTAGCA CACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGGAGGTTTGAC CTTGTTGATACCCTATCTTCTGACAACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTATTCATTGGT GGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTACTCAGTAAATTCCGAATAGACT TCTCCGATATCATGGTCCTAGGAGACATCAACACCAAACCAAAAAAGGAAAACATCGTCGCTTTTGATGA CATGATTGAGCCATACAGACTTCACGAGGATGATAAAGAGCAGGATATTGCAGACAAAATGAAAGAGGAT GAGCCCTGGCGCATCACAGACAACGAGCTGGAGCTCTACAAGACCAAGACATATCGGCAGATCAGGTTAA ATGAATTATTAAAGGAGCATTCAAGCACAGCTAATATTATTGTCATGAGTCTTCCAGTTGCCCGGAAGGG TGCTGTGTCCAGCGCGCTGTACATGGCTTGGTTAGAAGCTCTGTCTAAGGACCTGCCACCAATCCTGCTG GTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214390 representing NM_031798 Red=Cloning site Green=Tags(s) MEPGPAAPSSGAPRPARDGDAPLTAAGVDLPGTAVLSGREDATAGSQAGGGVRGEGTPAAGDGLGKPLGP TPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAGAAGKETPAAGKAGTESGVAKGSEEAKGRFRVNFVDPA ASSSADDSLSDAAGVGGDGPNVSSQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMD AVPRIDHYRHTAAQLGEKLLRPSLGEFHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIK GVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRS LGPEFGGAIGLIFAFPNAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVVLLGISVAGME WEAKAQIVLLVILLLAIADFVIGTFISLDSKKPKGFFGYKSEIFSENFGPDFREEETFFSVFAIFFPAAT GILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACK LNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQ MFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFK YYNMWISLTGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSG VEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKN KMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVV IRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDAFKASGEKPITQKDEEEDGKTSTQPL LKKESKGPVAPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIG GKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDDMIEPYRLHEDDKEQDIADKMKED EPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILL VRGNHQSVLTFYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031798
ORF Size	3609 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031798.1, NP_113986.1
RefSeq Size	6402 bp
RefSeq ORF	3612 bp
Locus ID	83629
MW	130.4 kDa
Gene Summary	Na/K/2Cl cotransporter involved in maintainence of intracellular Cl(-) levels [RGD, Feb 2006]

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