Cry1 (NM_198750) Rat Untagged Clone

CAT#: RN214434

Cry1 (untagged ORF) - Rat cryptochrome 1 (photolyase-like) (Cry1), (10 ug)



  "NM_198750" in other vectors (3)

CNY 7,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC124541
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214434 representing NM_198750
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTGAACGCCGTGCATTGGTTCCGAAAGGGACTCCGGCTGCACGACAACCCCGCCCTGAAGGAGT
GCATCCAGGGCGCCGACACCATCCGCTGCGTGTACATCCTCGACCCCTGGTTCGCCGGCTCTTCCAACGT
GGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAGGACCTTGATGCCAATCTACGAAAGTTAAAT
TCTCGCCTGTTTGTGATCCGGGGACAGCCAGCTGACGTGTTTCCGAGGCTTTTCAAGGAATGGAATATTA
CTAAACTTTCAATTGAGTACGATTCTGAGCCTTTTGGGAAGGAACGAGATGCGGCCATCAAGAAGCTGGC
CACTGAGGCTGGTGTGGAAGTCATCGTGCGCATTTCACACACACTGTATGACCTAGACAAGATCATAGAA
CTCAATGGTGGGCAGCCACCCCTAACATACAAAAGGTTTCAGACTCTCGTCAGCAAAATGGAGCCCCTGG
AGATGCCAGCAGACACCATCACATCAGACGTGATAGGGAAGTGCACAACCCCTCTGTCCGACGACCATGA
TGAGAAGTACGGAGTTCCTTCCCTGGAGGAGCTCGGTTTTGATACCGATGGCTTGTCCTCTGCGGTGTGG
CCAGGAGGAGAAACTGAGGCACTTACACGTTTGGAAAGGCATTTGGAAAGAAAGGCCTGGGTGGCAAACT
TTGAAAGACCTCGAATGAATGCAAACTCCCTGCTCGCAAGCCCAACTGGACTGAGTCCTTATCTCCGCTT
CGGTTGTTTGTCATGTCGGCTATTTTATTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAAAATAGT
TCCCCTCCCCTTTCTCTTTATGGGCAACTCCTGTGGCGTGAATTTTTTTATACAGCAGCCACAAACAACC
CACGCTTTGACAAAATGGAAGGGAACCCCATCTGTGTTCAAATCCCTTGGGACAAGAATCCCGAGGCTCT
GGCCAAATGGGCAGAAGGCCGGACAGGCTTTCCGTGGATTGACGCCATCATGACACAGCTTCGTCAGGAG
GGCTGGATCCACCATCTAGCCCGACATGCAGTTGCCTGTTTCCTGACCCGCGGCGACCTATGGATCAGTT
GGGAAGAAGGGATGAAGGTCTTTGAAGAATTACTGCTTGACGCCGACTGGAGCATCAATGCTGGGAGTTG
GATGTGGCTGTCCTGCAGTTCCTTTTTCCAACAGTTTTTCCACTGCTACTGCCCTGTTGGTTTTGGTAGG
AGGACAGACCCCAATGGAGACTATATTAGGCGTTATTTGCCTGTCCTAAGAGGCTTCCCTGCAAAATATA
TCTACGATCCTTGGAATGCACCAGAAGGTATCCAGAAGGTAGCCAAATGTTTGATAGGCGTTAATTATCC
CAAACCAATGGTGAACCATGCCGAGGCAAGTCGACTGAATATTGAAAGAATGAAGCAGATCTATCAGCAG
CTCTCCCGCTACAGAGGGCTAGGCCTCCTTGCCTCAGTCCCTTCTAATCCTAATGGGAACGGAGGGCTCA
TGGGCTATGCTCCTGGAGAGAATGTCCCCAGCGGTGGCAGTGGTGGCGGAAACTGCTCTCAAGGAAGTGG
TATTTTACACTATGCTCACGGAGACAGTCAGCAAACTAACCCACTAAAGCAAGGAAGAAGCTCCATGGGC
ACTGGCCTCAGCAGCGGGAAGCGCCCAAGTCAGGAAGAGGACGCACAGAGTGTTGGCCCCAAAGTCCAGC
GACAGAGCAGTAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_198750
Insert Size 1767 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198750.2, NP_942045.2
RefSeq Size 2938 bp
RefSeq ORF 1767 bp
Locus ID 299691
UniProt ID Q32Q86
Gene Summary This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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