Scn5a (NM_013125) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nav1.5; SCAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214460 representing NM_013125 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACCTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA TCGAGAAGCGAATGGCTGAAAAGCAAGCCCGAGGAGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCT GCAGGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGC AACCCACCCCGAGAGCTCATCGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCT TCATCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTATGTCCTCAGCCC CTTCCACCCCGTGCGCCGAGCGGCCGTGAAGATCCTGGTACACTCGCTCTTTAGCATGCTCATCATGTGC ACCATCCTGACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCGCCTTGGACCAAATATGTTGAGTACA CCTTCACTGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCATT CACCTTCCTTCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCATACACAACTGAATTT GTGGACCTGGGCAATGTCTCAGCCTTACGCACCTTCCGAGTCCTCCGGGCCCTGAAAACTATATCGGTCA TTTCAGGCCTGAAGACCATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAACTGGCCGATGTGATGGTCCT CACTGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAG TGTGTGCGTAACTTCACCGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGACGGCCTAGTCTGGAACT CCCTGGACGTCTACCTCAATGACCCAGCCAATTACCTGCTCAAGAATGGCACCACGGATGTGTTACTATG TGGGAACAGCTCTGATGCCGGGACATGCCCTGAGGGCTATCGGTGCCTGAAGGCAGGTGAGAACCCAGAC CACGGTTACACCAGCTTCGACTCCTTCGCCTGGGCCTTCCTTGCACTCTTCCGCCTGATGACACAGGACT GCTGGGAACGCCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTCGT CATCTTTCTGGGCTCCTTCTACCTGGTGAACTTGATCCTGGCTGTGGTGGCCATGGCCTACGAGGAGCAA AACCAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGCTTCCAGGAGGCCATGGAGATGCTCAAGA AGGAACACGAGGCTCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTT GGCCCCAGTAACCAACCATGAGAGAAAGAGCAAAAGGAGGAAACGACTATCTTCGGGGACAGAGGATGGT GGGGATGACAGGCTCCCCAAGTCGGACTCAGAAGATGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCC ATGGGCTCAGCCGGACATCCATGAGGCCCCGCTCGAGCCGAGGGAGCATTTTCACGTTCCGAAGACGGGA CCAAGGCTCTGAGGCGGACTTCGCAGATGACGAGAACAGCACTGCGGGGGAGAGCGAGAGCCACCGCACA TCGCTGCTGGTACCCTGGCCCCTGCGCCATCCCAGCGCCCAAGGACAGCCCGGCCCTGGAGCCTCAGCTC CCGGTTACGTTCTCAATGGCAAAAGGAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGC AGGTGACGCAGAGGCCACCTCCCCAGGGAGCTACCTTCTCCGCCCTATGGTGCTGGACCGACCCCCAGAC ACGACCACTCCGTCAGAGGAGCCCGGTGGGCCCCAGATGCTGACACCTCAGGCTCCGTGTGCAGATGGTT TTGAGGAGCCCGGAGCACGGCAACGGGCACTCAGCGCTGTCAGTGTCCTCACCAGCGCCCTGGAAGAGTT GGAGGAGTCCCATCGGAAGTGTCCACCATGCTGGAACCGCTTTGCCCAGCACTACCTCATCTGGGAGTGC TGTCCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTTGTGGTCATGGACCCATTTGCCGACCTCACTA TCACCATGTGCATCGTGCTCAATACGCTCTTCATGGCTCTGGAGCATTACAACATGACGGCAGAGTTTGA GGAGATGCTGCAGGTCGGAAACCTGGTCTTCACGGGAATCTTCACAGCGGAGATGACCTTCAAGATCATC GCCCTTGACCCCTACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGTC TCATGGAGCTGGGGCTGTCCCGCATGGGCAACTTGTCTGTGCTACGTTCCTTCCGTCTGCTGCGGGTCTT CAAGCTGGCCAAGTCCTGGCCCACCCTGAACACGCTCATCAAGATCATCGGAAACTCCGTGGGCGCCCTG GGGAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTCTTCGGCA AGAACTACTCAGAACTGAGGCACCGCATCAGCGACTCCGGCCTGCTGCCCCGCTGGCACATGATGGACTT TTTCCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATG GAGGTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTCGTCATGGTCATTGGCAACCTTGTGGTCC TGAATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGG GGAGATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACC TGGGACTTCTGCTGCGGGATCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGC TGCCCAGCTGTATCACCGCCCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGTGCCCCCAGCCCGCAA GGAAACACGATTCGAGGAGGACAAGCGACCCGGCCAGGGCACCCCTGGGGATTCGGAGCCTGTGTGTGTG CCCATCGCCGTGGCTGAGTCAGACACTGAAGACCAGGAAGAGGATGAAGAGAACAGCCTTGGCACAGAGG AAGAGTCCAGCAAACAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCTACCAGGAGCCCAGGGCCTG GAGCCAGGTGTCAGAGACCACGTCCTCTGAAGCTGGGGCCAGTACATCTCAGGCAGACTGGCAGCAAGAG CAGAAAACGGAGCCCCAGGCCCCGGGGTGCGGTGAGACCCCTGAGGACAGTTACTCCGAGGGCAGCACAG CTGACATGACCAACACCGCCGACCTCCTGGAGCAAATCCCAGACCTTGGTGAGGACGTCAAGGACCCAGA GGACTGCTTTACTGAAGGCTGCGTCCGACGCTGTCCCTGCTGCATGGTAGACACAACCCAGTCCCCAGGG AAGGTCTGGTGGCGATTGCGCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTCGAGACTTTCATCA TCTTCATGATCCTGCTCAGCAGTGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGACCAT CAAGGTTCTGCTGGAGTACGCGGACAAGATGTTCACCTACGTCTTTGTGTTGGAGATGCTGCTCAAGTGG GTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTGATTGTGGACGTCT CGCTGGTCAGCCTCGTGGCAAACACCTTAGGCTTCGCCGAAATGGGTCCCATCAAGTCACTGAGGACACT GCGTGCACTTCGACCCCTGAGGGCCTTGTCGAGATTTGAGGGCATGCGGGTGGTGGTCAATGCGCTGGTG GGCGCCATCCCCTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGG GCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACAGAAGGGGACCTGCCTCTGAACTA CACCATCGTGAACAACAAGAGTGAGTGCGAGTCCTTCAACGTGACCGGAGAGTTGTACTGGACCAAGGTG AAGGTCAACTTTGACAACGTGGGAGCCGGGTACCTGGCCCTCCTGCAGGTGGCGACATTTAAAGGCTGGA TGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAGGAGCAGCCGCAGTGGGAAGACAACCTCTA CATGTACATCTACTTTGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCATCGGTGTC ATCATTGACAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGATATCTTCATGACGGAGGAGCAGA AGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCTTGAA CAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTCACCATCATGTTCCTCATC TGTTTGAACATGGTGACCATGATGGTGGAGACAGATGACCAGAGCCCTGAGAAGGTCAACATCTTGGCCA AGATCAACCTGCTCTTCGTGGCCATCTTCACAGGCGAGTGTATTGTCAAGATGGCTGCCCTGCGCCACTA TTACTTCACCAACAGCTGGAACATCTTCGACTTTGTGGTGGTCATCCTCTCCATTGTTGGCACTGTCCTC TCCGACATCATCCAGAAGTACTTCTTCTCCCCGACACTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCC GCATCCTCAGGCTGATCCGCGGAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCC CGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAAC TTCGCTTACGTCAAGTGGGAGGCCGGCATCGATGACATGTTCAACTTCCAGACCTTCGCCAACAGCATGC TGTGCCTGTTCCAGATCACCACATCAGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCC TCCCTACTGCGACCCCAACCTGCCCAACAGCAACGGCTCCCGGGGGAACTGTGGGAGCCCGGCGGTGGGC ATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCCATCATCC TCGAGAACTTCAGCGTGGCCACCGAGGAGAGCACAGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTA TGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATCTGGCCCTGTCCGACTTTGCA GATGCCTTGTCTGAGCCGCTCCGCATCGCCAAACCCAACCAGATAAGCCTCATCAACATGGATCTGCCCA TGGTGAGCGGAGACCGTATCCACTGTATGGACATACTGTTCGCTTTCACCAAGAGGGTGCTCGGCGAGTC TGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAGTTCATGGCGGCCAACCCTTCCAAGATCTCC TACGAGCCCATCACCACCACCCTGAGGAGAAAGCACGAGGAGGTGTCGGCCACGGTCATCCAGCGTGCCT TCCGGAGGCACCTGCTGCAGCGCTCGGTGAAGCATGCCTCCTTTCTCTTCCGCCAGCAAGCGGGCGGCAG TGGCCTCTCCGACGAGGATGCCCCTGAGCGGGAGGGCCTCATCGCCTACATGATGAATGGGAACTTCTCT CGGCGCAGTGCTCCGCTCTCCAGCTCCTCCATCTCCTCCACGTCCTTCCCCCCGTCCTACGACAGCGTCA CGAGAGCCACCAGTGATAACCTCCCGGTGCGTGCGTCTGACTATAGCCGCAGCGAAGATCTTGCAGACTT CCCTCCATCTCCAGATAGGGACCGAGAGTCTATCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013125
Insert Size	6060 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013125.2, NP_037257.1
RefSeq Size	8468 bp
RefSeq ORF	6060 bp
Locus ID	25665
UniProt ID	P15389
Gene Summary	acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.