Plce1 (NM_053758) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Plce
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214972 representing NM_053758 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCGAAGAAATGGCCGCTTCCTTCCTCATCCCGGTGCCTCAAAGGAAAGTGGCTTCTGCCCAGT CAGTGGCAGAGGAGCGGGGTGAAAAGGTCTCAGAAGCCGGCATTCCTAAGACACGAGCTGGCAGACAGGG TGGGCTCACCCCTCGTACCATCTCACAGCGGAACGAACCTGAGGAAGAGTCTCCTAGAACTGATTTTTCC CAGGTCTTCTCAATAGCGAGGGGGGAACTAGACAGCGACGAGAATCACAATGAAAGGTGCTGGGAGGAAA ATGTGCCAGGTTCCACGAAAAACCACGCGGTTAACTGCAACAGCTTATTGCAAAGCCACCAGCATGCGCT TCCCCCGAGCCAGCTCTGTGAAGTCTGTGACTCTGTCACAGAAGAACACCTGTGTTTGCAGCCTGGGATT CCTTCTCCACTGGAAAGGAAGGTGTTTCCTGGGATCGAACTGGAGATGGAGGACTCTCCCATGGACGTGA GCCCTTTGGGAAACCAGCCTGGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACATGGCGGTATT TCATTTCCATTATGCAGGTGACAGGACAATGCCAGGTGCTTTCCATACCCTGTCAGAAAAATTCATTTTG GATGACTGTGCAAACTGTGTTACTCTTCCTGGGGGGCAGCAAAACAAAAATTACATGGCGTATACTTGCA AACTGGTGGAGTTGACAAGAACCTGTGGTAGTAAGAATGGGCAGCTGAAGTGTGACCACTGCACCTCGCT GCGGGATGAATACCTGTGCTTTGAAAGCTCCTGCCGGAAGGCTGAGGCTCTCTCCTCAGGTGGTGGCTTT TGCGAGGACGGATTTACTCATGGTCCCTCTGCCAAGACTTTTCTGAACCCTTTGGAGGAATTCTCTGATA ATTGTGAAGACGTGGACGATATTTTTAAAGGCAAAAAGGAGCGGTCCACTTTGTTAGTCCGAAGGTTTTG TAAAAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACAAGGGCCATTGTGAGAACCCTGCCTTCT GGCCACATTGGGCTGGCTGCTTGGAGTTACGTCGATCAGAAGAAAGCTGGTCTCATGTGGCCTTGTGGGA ATGGAATGAGACCTCTGTCCACAGTGGACGTAAGGCAAAGTGGGAGACAGCGTCTGTCTGAAGCCCAGTG GTGTCTGATCTACAGTGCCGTGAGAAGAGAAGAGACAGAAGATACGGTTGGCTCTCTTCTGCATTGCTCC ACACAGCTCCCAACCCCTGACACAGCCCATGGAAGGATCGGAGATGGTCCCTGCCTAAAGCAATGTGTGC GAGACAGCGAGTGTGAGTACCGTGCCACCCTCCAAAGGACGTCCATAGCTCAGTACATCACCGGTTCTCT CCTAGAGGCAACCACATCTTTAGGAGCAAGAAGCAGCCTTCTCAGTTCCTTTGGAGGATCCACTGGGCGA ATAATGCTGAAAGAACGCCAACCGGGCACTTCTATGGCCAATTCCAGTCCTGTTCCTTCAAGTTCAGCTG GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC AGAACAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGTTTCTTAACCCGGGACTTG AGTTCCCCCGAGTGTCAGAGATCACTGCCCCGTCTCAAAGCATGCATCTCAGAATCAATCCTCATGTCTC AAAGTGGAGAGCACAATGCCCTTGAAGACCTCGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG GCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA TGCTGCTGGAACATGGGCAACTACAATGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC TGAAGATGTGGCAGTTCATGGACCAGTCTGACATCGAGACCATGAGGAGCCTGAAAGACGCCATGGCTCA GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTT CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT GCCCACGATACAGCTCCCAAGAAGAAGCCCTGGAGTTTGTAGCGGATTACAGTGGACAAGATAACTTCTT ACAGCGAGTGGGGCAGAATGGCTTGAAGAATCCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGATC ATCCGGAGCTGCAGCCGAAGCTTGGAGACGGAGGACGAGGAGAGCGCCAGCGAAGGGAGCGGCTCTAGGA AAAACTCCCTCAAGGACAAAACCCGGTGGCAGTTTATAATTGGAGATTTGCTGGATTCAGACAATGACAT CTTTGAGAAGTCCAAAGAGTGCGACCCTCATGGATCAGAGGAGTCACAGAAAGCCTTTGACCATGGGACA GAGCTCATCCCATGGTATGTGCTGTCCATCCAAGCGGATGTGCACCAGTTCCTCCTGCAGGGGGCCACAG TCATCCACTATGACCAGGACACACATCTCTCCGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGTAC CTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCTGGTGCCAGACTCAAGCTCGGTGTGCTTAGTAAC GTGGCCGAACCTGGCAAGTTCCCATCACTGGGCAACGCTGGGGTGAGCGGCCTGGTAGAGGGAATCCTGG ATCTGTTTTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA CAAACTTAGTAGCATGCTTCTGTCAGAGACTGGGGTGACGCTGCTCTATGGGCTTCAGACCACCGACAAT AGATTACTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTTTTCAGTGGATTGCTGGAACTCACTA CAGCTGTGAGAAAGATACGGAAGTTCCCTGACCAAAGACAACAGTGGCTTCGGAAACAGTATGTCAGCTT CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGAGA TGGAGCACTAGAAACCCCAGCCCTGGGATGTCAGCAAAGAATGCCGAGAAGCCCAACATGCAGAGAAACA ACACCCTAGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGGGAGAGTGGAGAGGTGAC GGACGATGAGATGGCCACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT CAAGAGGCTAATGAACAGGAAGACTCAGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA GAAGAGCCTACTCATTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCTATCAGTGC CTGGAGTAGCAGCAGCTGGCATGGGCGGATCAAAGGAGGAATGAAAGGCTTCCAGAGCTTCATGGTCTCA GACAGTAACATGAGTTTCATTGAATTCGTCGAGCTGTTCAAATCATTCAGCATAAGGAGCCGCAAGGACT TGAAGGACATCTTTGACATCTACTCTGTACCCTGTAATCGGTCTGCTTCTGAGTCAACCCCACTGTACAC CAACCTGACAATCGAAGAAAATACCAATGACCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC GACTTGGGGCTGTTCATGAAGAGTAAGCAGCAGCTTTCTGACAACCAGAGGCAGATATCTGACGCCATTG CTGCTGCAAGCATTGTGACTAATGGCACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT ACTCCAGCTCAACGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTCAGCATC ATCCAGAAGTTCGAGCCCAACATCAGCATGTGTCATCAAGGTCTATTGTCCTTCGAAGGGTTTGCAAGGT TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGACGAGTCGCGGGAGAACAAGAAAGACCTGCAGCT ACCCCTCTCCTACTATTACATCGAATCTTCGCACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG TCCTCTGTGGAGCTCTACAGCCAGGTCCTCTTGCAAGGATGCAGAAGCATAGAGCTGGACTGCTGGGATG GAGATGATGGGATGCCCATTATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGT GGAAGCCATCGATCGCAGTGCCTTCATCACCTCCGACCTGCCAATCATCATATCCATTGAGAACCATTGC TCATTGCCTCAGCAACGGAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT TCTTGTTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGACCAACTTAGAAGGAAAGTGCT TCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTCAAACAAAAGGCTCATCAGTTA GCATCCATGCAAACACAGGCTTTCACTGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG AAGACGAGGAGGACGAATATGATTATGATTACGAGTCCCTGTCTGATGACAACATTCTGGAAGACAGACC TGAGAACAAATCATGTGCCGACAAGCTTCAGTTTGAGTACAATGAAGAAGTCCCCAAGAGGATAAAGAAA GCAGACAACTCCTCCGGCAATAAGGGAAAGGTCTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC AGAACAAAAAAGAAAGCAGGCAGATTGCACCGGAGCTCTCTGACCTCGTCATCTATTGCCAGGCGGTAAA ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTGGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC ATTTTTGGCAACAATCCCGGCAGAATGAGCCCAGGGGAGACAGCGTCATTTAACAGAACATCTGGAAAAA GTTCTTGTGAAGGAATTCGACAAATCTGGGAGGAACCTCCACTCAGCCCAAACACATCCCTCAGCGCCAT CATTAGAACTCCTAAATGCTATCATATCTCGTCACTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGG TATTCTCAGAAACTGATCCAGCACACTGCTTGCCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAATCG ACTCATCCAATCCCAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCAGAC GGATGACCTTCCTTTACATTTAAACGCTGCCATGTTTGAGGCGAATGGTGGGTGTGGCTATGTTTTGAAG CCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTATCAGAAGTTTTCACCACTGGAAAGAGACCTGGACG CCATGGATCCTGCTACCTATTCTTTAACCATCATCTCTGGCCAGAATGTGTGTCCTAGTAACAGCACCGG AAGTCCGTGCATTGAAGTTGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCCATC CACCGAAACACTCTGAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTGTAT TTCTTCGTTTTGCAGTTGTGGAGAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAAAGC TTTAAAGAGAGGATATCGGCATCTTCAGCTGCGGAACCTCCACAACGAGATCTTGGAAATCTCTAGCTTA TTCATAAACAGCAGGAGGATGGAAGATAATCCCTCTGGCAGTACCCGGCCAGCCTCTTTGATGTTCAATA CTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTTTGC TGTTTTCACAATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGATTTTGGCAGTTGACCAGGAC ACCAAACTTACAGCCGCAGACTATTTTCTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATGAAT GCAGGAAACAACCATTCCAGAGAGCTGTGGGCCCAGAGGAAGATATTGTGCAGATTTTAAACAGCTGGTT CCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAAGAAACCCTAGAAGAGAAAAACATT GTCCACGATGACAGAGAGGTGATCTTGAGCTCAGAAGAGGAGAGTTTCTTTGTCCAAGTGCACGATGTCT CTCCAGAGCAGCCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTGATTCAGCAGAC CTTGTGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAATCCGTGTGACTATGTGCTTTTGGAA GAGGTAATGAAAGATGCACCCAATAAGAAGTCTTCTACCCCGAAGTCCTCACAGAGGATCCTCTTGGACC AAGAATGTGTGTTTCAAGCCCAAAGCAAGTGGAAGGGTGCAGGGAAATTCATCCTTAAGCTAAAGGAGCA GGTGCAGGCATCCAGAGAAGATAAAAGGAGGGGCATCTCCTTTGCAAGTGAACTCAAGAAACTCACGAAG TCCACTAAGCAAACCCGAGGACTCACCTCGCCTCCTCAGCTTGTGGCCTCAGAAAGTGTGCAAAGCAAGG AGGAGAAACCCATGGGTGCCTTGGCCTCTGGTGACACAGCGGGCTACCAGTCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053758
Insert Size	6846 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053758.2, NP_446210.1
RefSeq Size	8355 bp
RefSeq ORF	6846 bp
Locus ID	114633
UniProt ID	Q99P84
Gene Summary	one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.