Plce1 (NM_053758) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Plce
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214972 representing NM_053758 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCGAAGAAATGGCCGCTTCCTTCCTCATCCCGGTGCCTCAAAGGAAAGTGGCTTCTGCCCAGT CAGTGGCAGAGGAGCGGGGTGAAAAGGTCTCAGAAGCCGGCATTCCTAAGACACGAGCTGGCAGACAGGG TGGGCTCACCCCTCGTACCATCTCACAGCGGAACGAACCTGAGGAAGAGTCTCCTAGAACTGATTTTTCC CAGGTCTTCTCAATAGCGAGGGGGGAACTAGACAGCGACGAGAATCACAATGAAAGGTGCTGGGAGGAAA ATGTGCCAGGTTCCACGAAAAACCACGCGGTTAACTGCAACAGCTTATTGCAAAGCCACCAGCATGCGCT TCCCCCGAGCCAGCTCTGTGAAGTCTGTGACTCTGTCACAGAAGAACACCTGTGTTTGCAGCCTGGGATT CCTTCTCCACTGGAAAGGAAGGTGTTTCCTGGGATCGAACTGGAGATGGAGGACTCTCCCATGGACGTGA GCCCTTTGGGAAACCAGCCTGGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACATGGCGGTATT TCATTTCCATTATGCAGGTGACAGGACAATGCCAGGTGCTTTCCATACCCTGTCAGAAAAATTCATTTTG GATGACTGTGCAAACTGTGTTACTCTTCCTGGGGGGCAGCAAAACAAAAATTACATGGCGTATACTTGCA AACTGGTGGAGTTGACAAGAACCTGTGGTAGTAAGAATGGGCAGCTGAAGTGTGACCACTGCACCTCGCT GCGGGATGAATACCTGTGCTTTGAAAGCTCCTGCCGGAAGGCTGAGGCTCTCTCCTCAGGTGGTGGCTTT TGCGAGGACGGATTTACTCATGGTCCCTCTGCCAAGACTTTTCTGAACCCTTTGGAGGAATTCTCTGATA ATTGTGAAGACGTGGACGATATTTTTAAAGGCAAAAAGGAGCGGTCCACTTTGTTAGTCCGAAGGTTTTG TAAAAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACAAGGGCCATTGTGAGAACCCTGCCTTCT GGCCACATTGGGCTGGCTGCTTGGAGTTACGTCGATCAGAAGAAAGCTGGTCTCATGTGGCCTTGTGGGA ATGGAATGAGACCTCTGTCCACAGTGGACGTAAGGCAAAGTGGGAGACAGCGTCTGTCTGAAGCCCAGTG GTGTCTGATCTACAGTGCCGTGAGAAGAGAAGAGACAGAAGATACGGTTGGCTCTCTTCTGCATTGCTCC ACACAGCTCCCAACCCCTGACACAGCCCATGGAAGGATCGGAGATGGTCCCTGCCTAAAGCAATGTGTGC GAGACAGCGAGTGTGAGTACCGTGCCACCCTCCAAAGGACGTCCATAGCTCAGTACATCACCGGTTCTCT CCTAGAGGCAACCACATCTTTAGGAGCAAGAAGCAGCCTTCTCAGTTCCTTTGGAGGATCCACTGGGCGA ATAATGCTGAAAGAACGCCAACCGGGCACTTCTATGGCCAATTCCAGTCCTGTTCCTTCAAGTTCAGCTG GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC AGAACAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGTTTCTTAACCCGGGACTTG AGTTCCCCCGAGTGTCAGAGATCACTGCCCCGTCTCAAAGCATGCATCTCAGAATCAATCCTCATGTCTC AAAGTGGAGAGCACAATGCCCTTGAAGACCTCGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG GCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA TGCTGCTGGAACATGGGCAACTACAATGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC TGAAGATGTGGCAGTTCATGGACCAGTCTGACATCGAGACCATGAGGAGCCTGAAAGACGCCATGGCTCA GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTT CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT GCCCACGATACAGCTCCCAAGAAGAAGCCCTGGAGTTTGTAGCGGATTACAGTGGACAAGATAACTTCTT ACAGCGAGTGGGGCAGAATGGCTTGAAGAATCCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGATC ATCCGGAGCTGCAGCCGAAGCTTGGAGACGGAGGACGAGGAGAGCGCCAGCGAAGGGAGCGGCTCTAGGA AAAACTCCCTCAAGGACAAAACCCGGTGGCAGTTTATAATTGGAGATTTGCTGGATTCAGACAATGACAT CTTTGAGAAGTCCAAAGAGTGCGACCCTCATGGATCAGAGGAGTCACAGAAAGCCTTTGACCATGGGACA GAGCTCATCCCATGGTATGTGCTGTCCATCCAAGCGGATGTGCACCAGTTCCTCCTGCAGGGGGCCACAG TCATCCACTATGACCAGGACACACATCTCTCCGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGTAC CTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCTGGTGCCAGACTCAAGCTCGGTGTGCTTAGTAAC GTGGCCGAACCTGGCAAGTTCCCATCACTGGGCAACGCTGGGGTGAGCGGCCTGGTAGAGGGAATCCTGG ATCTGTTTTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA CAAACTTAGTAGCATGCTTCTGTCAGAGACTGGGGTGACGCTGCTCTATGGGCTTCAGACCACCGACAAT AGATTACTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTTTTCAGTGGATTGCTGGAACTCACTA CAGCTGTGAGAAAGATACGGAAGTTCCCTGACCAAAGACAACAGTGGCTTCGGAAACAGTATGTCAGCTT CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGAGA TGGAGCACTAGAAACCCCAGCCCTGGGATGTCAGCAAAGAATGCCGAGAAGCCCAACATGCAGAGAAACA ACACCCTAGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGGGAGAGTGGAGAGGTGAC GGACGATGAGATGGCCACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT CAAGAGGCTAATGAACAGGAAGACTCAGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA GAAGAGCCTACTCATTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCTATCAGTGC CTGGAGTAGCAGCAGCTGGCATGGGCGGATCAAAGGAGGAATGAAAGGCTTCCAGAGCTTCATGGTCTCA GACAGTAACATGAGTTTCATTGAATTCGTCGAGCTGTTCAAATCATTCAGCATAAGGAGCCGCAAGGACT TGAAGGACATCTTTGACATCTACTCTGTACCCTGTAATCGGTCTGCTTCTGAGTCAACCCCACTGTACAC CAACCTGACAATCGAAGAAAATACCAATGACCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC GACTTGGGGCTGTTCATGAAGAGTAAGCAGCAGCTTTCTGACAACCAGAGGCAGATATCTGACGCCATTG CTGCTGCAAGCATTGTGACTAATGGCACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT ACTCCAGCTCAACGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTCAGCATC ATCCAGAAGTTCGAGCCCAACATCAGCATGTGTCATCAAGGTCTATTGTCCTTCGAAGGGTTTGCAAGGT TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGACGAGTCGCGGGAGAACAAGAAAGACCTGCAGCT ACCCCTCTCCTACTATTACATCGAATCTTCGCACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG TCCTCTGTGGAGCTCTACAGCCAGGTCCTCTTGCAAGGATGCAGAAGCATAGAGCTGGACTGCTGGGATG GAGATGATGGGATGCCCATTATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGT GGAAGCCATCGATCGCAGTGCCTTCATCACCTCCGACCTGCCAATCATCATATCCATTGAGAACCATTGC TCATTGCCTCAGCAACGGAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT TCTTGTTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGACCAACTTAGAAGGAAAGTGCT TCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTCAAACAAAAGGCTCATCAGTTA GCATCCATGCAAACACAGGCTTTCACTGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG AAGACGAGGAGGACGAATATGATTATGATTACGAGTCCCTGTCTGATGACAACATTCTGGAAGACAGACC TGAGAACAAATCATGTGCCGACAAGCTTCAGTTTGAGTACAATGAAGAAGTCCCCAAGAGGATAAAGAAA GCAGACAACTCCTCCGGCAATAAGGGAAAGGTCTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC AGAACAAAAAAGAAAGCAGGCAGATTGCACCGGAGCTCTCTGACCTCGTCATCTATTGCCAGGCGGTAAA ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTGGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC ATTTTTGGCAACAATCCCGGCAGAATGAGCCCAGGGGAGACAGCGTCATTTAACAGAACATCTGGAAAAA GTTCTTGTGAAGGAATTCGACAAATCTGGGAGGAACCTCCACTCAGCCCAAACACATCCCTCAGCGCCAT CATTAGAACTCCTAAATGCTATCATATCTCGTCACTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGG TATTCTCAGAAACTGATCCAGCACACTGCTTGCCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAATCG ACTCATCCAATCCCAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCAGAC GGATGACCTTCCTTTACATTTAAACGCTGCCATGTTTGAGGCGAATGGTGGGTGTGGCTATGTTTTGAAG CCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTATCAGAAGTTTTCACCACTGGAAAGAGACCTGGACG CCATGGATCCTGCTACCTATTCTTTAACCATCATCTCTGGCCAGAATGTGTGTCCTAGTAACAGCACCGG AAGTCCGTGCATTGAAGTTGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCCATC CACCGAAACACTCTGAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTGTAT TTCTTCGTTTTGCAGTTGTGGAGAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAAAGC TTTAAAGAGAGGATATCGGCATCTTCAGCTGCGGAACCTCCACAACGAGATCTTGGAAATCTCTAGCTTA TTCATAAACAGCAGGAGGATGGAAGATAATCCCTCTGGCAGTACCCGGCCAGCCTCTTTGATGTTCAATA CTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTTTGC TGTTTTCACAATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGATTTTGGCAGTTGACCAGGAC ACCAAACTTACAGCCGCAGACTATTTTCTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATGAAT GCAGGAAACAACCATTCCAGAGAGCTGTGGGCCCAGAGGAAGATATTGTGCAGATTTTAAACAGCTGGTT CCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAAGAAACCCTAGAAGAGAAAAACATT GTCCACGATGACAGAGAGGTGATCTTGAGCTCAGAAGAGGAGAGTTTCTTTGTCCAAGTGCACGATGTCT CTCCAGAGCAGCCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTGATTCAGCAGAC CTTGTGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAATCCGTGTGACTATGTGCTTTTGGAA GAGGTAATGAAAGATGCACCCAATAAGAAGTCTTCTACCCCGAAGTCCTCACAGAGGATCCTCTTGGACC AAGAATGTGTGTTTCAAGCCCAAAGCAAGTGGAAGGGTGCAGGGAAATTCATCCTTAAGCTAAAGGAGCA GGTGCAGGCATCCAGAGAAGATAAAAGGAGGGGCATCTCCTTTGCAAGTGAACTCAAGAAACTCACGAAG TCCACTAAGCAAACCCGAGGACTCACCTCGCCTCCTCAGCTTGTGGCCTCAGAAAGTGTGCAAAGCAAGG AGGAGAAACCCATGGGTGCCTTGGCCTCTGGTGACACAGCGGGCTACCAGTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214972 representing NM_053758 Red=Cloning site Green=Tags(s) MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFS QVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGI PSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFIL DDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGF CEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPS GHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCS TQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGR IMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDL SSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAK CCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVV PFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQI IRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGT ELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSN VAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDN RLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRR WSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDP QEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVS DSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESTPLYTNLTIEENTNDLQPDLDLLTRNVS DLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSI IQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGE SSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHC SLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQL ASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKK ADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKS IFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRR YSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLK PPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPI HRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSL FINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQD TKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNI VHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLE EVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTK STKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053758
ORF Size	6843 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053758.2, NP_446210.1
RefSeq Size	8355 bp
RefSeq ORF	6846 bp
Locus ID	114633
UniProt ID	Q99P84
MW	255.4 kDa
Gene Summary	one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006]

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