Dock1 (NM_001143858) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1566072
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215029 representing NM_001143858 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCTGGGTCCCCACCAAACGAGAGGAGAAGTACGGCGTGGCTTTTTATAACTACGATGCCAGGG GAGCTGATGAATTGTCCTTACAAATAGGAGACACGGTGCACATCCTAGAAACTTATGAAGGGTGGTACAG AGGCTACACCTTAAGGAAAAAGTCTAAAAAGGGTATATTTCCTGCTTCGTATATTCATCTTAAAGAAGCC ATTGTTGAAGGAAAAGGGCAGCATGAAACTGTCATCCCCGGGGACCTCCCCCTCATACAGGAAGTCACCA CAACGCTCCGAGAGTGGTCTACCATCTGGAGACAACTTTATGTGCAAGATAATAGGGAGATGTTTCGAAG TGTTCGGCACATGATCTATGACCTTATTGAGTGGCGGTCGCAGATTCTTTCTGGAACTCTGCCCCAGGAT GAGCTCAAAGAGCTGAAGAAGAAGGTCACAGCCAAAATCGATTATGGAAACAGAATTCTTGATTTGGACT TGGTGGTGAGGGATGAAGATGGGAACATTTTGGATCCGGAACTAACTAGCACCATCAGTCTCTTCCGAGC ACATGAAGTTGCTTCTAAACAAGTAGAAGAGAGACTGCAAGAGGAGAAATCTCAAAAACAGAATATGGAT ATCAACAGACAAGCCAAGTTCGCAGCCACCCCATCTCTGGCCTTGTTTGTTAACCTCAAAAATGTGGTTT GTAAAATAGGAGAAGATGCCGAAGTCCTCATGTCTCTTTATGACCCTGTGGAGTCCAAATTCATCAGTGA GAACTACCTGGTCCGCTGGTCAAGTTCAGGATTACCAAAAGACATTGATAGGTTACATAACTTGCGTGCG GTGTTTACGGATCTGGGGAGCAAAGACCTGAAAAGGGAGAAAATTAGTTTCGTCTGCCAGATTGTCCGAG TTGGTCGTATGGAGCTGAGGGACAGCAACACGAGGAAACTGACTTCGGGGCTCCGGCGACCTTTTGGCGT GGCTGTGATGGACGTAACAGACATCATAAACGGGAAAGTGGACGATGAAGATAAACAGCACTTCATCCCC TTCCAGCCGGTGGCAGGGGAGAATGACTTCCTTCAAACTGTCATAAACAAAGTCATTGCAGCCAAAGAAG TCAACCACAAGGGACAAGGTTTGTGGGTAACCTTGAAATTACTTCCTGGAGACATCCATCAGATTCGGAA GGAGTTTCCTCACTTGGTGGACAGGACCACAGCTGTGGCCCGCAAGACTGGGTTTCCAGAGATCATCATG CCAGGTGATGTTCGAAATGATATCTATGTGACATTAGTTCAAGGGGATTTTGATAAAGGCAGTAAAACAA CCGCGAAGAATGTGGAGGTGACGGTGTCTGTCTATGATGAAGATGGGAAGCGATTGGAGCACGTGATCTT CCCCGGTGCTGGCGACGAGGCCATCTCTGAGTACAAATCAGTGATTTATTACCAAGTGAAGCAGCCTCGT TGGTTCGAGACCCTTAAGGTGGCCATCCCCATCGAGGACGTGAACCGGAGTCACCTTCGATTTACCTTCC GCCATAGATCCTCTCAGGACTCCAAGGATAAATCTGAGAAGATCTTCGCACTGGCCTTTGTGAAGCTGAT GAGATATGATGGGACCACCCTGAGAGACGGGGAGCATGACCTTATTGTCTACAAGGCTGAAGCCAAAAAG CTGGAAGACGCTGCCACATATTTGAGCCTCCCTAGTACCAAGGGAGAGTTGGAAGAAAAGGGCCACTCAG CCACAGGCAAAGGAATGCAGAGTCTTGGGAGTTGCACCATCAGTAAAGATTCTTTCCAGATCTCTACGCT GGTGTGCTCCACCAAACTGACCCAGAATGTGGACCTCCTGGGGCTGTTGAAGTGGAGATCCAACACCAAC CTTCTGCAGCAGAACTTGAGACAGTTGATGAAGGTGGATGGTGGCGAAGTGGTAAAGTTCCTGCAGGACA CCTTGGATGCCCTCTTCAACATCATGATGGAGAACTCCGAGAGTGAGACGTTTGATACCTTGGTGTTTGA TGCGCTGGTCTTTATCATTGGACTAATCGCGGATAGGAAGTTTCAGCATTTTAATCCTGTTTTGGAGACT TACATTAAGAAACACTTCAGTGCGACATTAGCCTACACGAAACTGACCAAGGTGTTAAGGACTTATGTGG CCAGCGCTGAAAAACCGGGAATCAACGAGCAGCTGTACAAAGCCATAAAAGCCTTGGAGTACATCTTCAA GTTCATAGTGCGCTCACGGGTACTGTTCAACCAGCTGTATGAAAACAAGGGGGAGGCCGACTTCGTGGAG TCCTTGTTGCAACTGTTCCGGTCCATCAACGACATGATGAGCAGCATGTCGGAGCTCACAGTCAGGGTGA AGGGGGCAGCATTGAAGTACTTGCCAACAATTGTCAATGATGTGAAGTTGGTGTTTGATCCCAAAGAGCT CAGTAAAATGTTCACGGAATTCATCTTGAATGTTCCTGTGGGCTTGCTGACTGTCCAGAAGCTCTACTGC CTGATTGAGATTGTTCACAGTGATCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTCCCCATGATGA CGGATCAGCTCAAGTACCACCTAGAGAGGCAGGAGGATCTGGAGGCATGCTGCCAGCTGCTTAGCAACAT CCTGGAGGTGCTATACCGGAAAGATGTGGGCCCGACTCAGAGACACGTCCAGATCATCATGGAGGAGCTT CTCAGGACTGTGAACAGGACGGTCATTTCCATGGGGCGGGATTCCGAACTCATTGGGAACTTTGTGGCTT GCATGACAGCTATTTTACGGCAGATGGAAGACTTCCATTATGCACACCTGATCAAGACCTTCGGGAAAAT GAGGACAGACGTCGTGGATTTTCTGATGGAGACATTCATCATGTTTAAGAACCTCATCGGGAAGAATGTC TACCCCTTTGACTGGGTGATAATGAACACGATGCAGAACAAAGTTTTCCTGCGGGCGATTAATCAGTATG CAGATATGCTGAACAAGAAATTTCTGGATCAAGCCAACTTTGAGCTGCAGCTGTGGAACAATTACTTTCA CCTGGCCGTGGCTTTCCTCACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCTAAGAGAGCCAAA ATCCTTAACAAGTATGGAGACATGAGGAGGCAGATTGGCTTTGAGATCAGAGACATGTGGTACAACTTGG GTCAACACAAGATAAAATTCATCCCAGAAATGGTTGGCCCTATATTGGAAATGACATTGATTCCTGAGAC AGAACTTCGAAAAGCCACCCTTCCCATCTTCTTCGACATGATGCAGTGTGAATTCCACTCCACCCGGAGC TTCCAAATGTTTGAAAATGAGATCATCACTAAGCTGGATCATGAAGTGGAAGGAGGCAGAGGAGACGAGC AATACAAAGTGTTATTTGATAAAATCCTCCTGGAGCACTGCAGGAAGCATAAATACCTGGCCAAGACAGG AGAAACTTTTGTGAAGCTCGTGGTGCGGTTGATGGAGAGACTCCTGGACTACAGGACCATCATGCACGAC GAAAACAAAGACAATCGTATGAGCTGCACAGTCAACGTGCTGAATTTCTACAAAGAGATTGAAAGAGAAG AGATGTATATAAGGTACTTGTATAAGCTCTGCGACCTGCACAAAGAGTGTGACAACTACACAGAGGCGGC TTACACCTTGCTTCTGCATGCGAAGCTTCTAAAGTGGTCAGAGGATGCGTGTGCAGCTCACCTCATTCAG CGGGACGGGTTCCAGGCGACAACACAGGGACAGCTGAAAGAACAGCTCTACCAAGAAATCATCCACTATT TTGACAAAGGCAAGATGTGGGAGGAGGCCATCGCCCTGGGCAAGGAACTTGCAGAGCAGTACGAAACGGA GTTGTTTGATTATGAGCAGCTGAGTGAGTTGCTGAAAAAACAGGCACAATTTTATGAGAACATCGTCAAG GTGATAAGACCCAAGCCGGACTATTTTGCTGTTGGCTACTATGGGCAAGGCTTCCCTTCATTCCTTCGGG GAAAAGTGTTCATTTACCGAGGAAAAGAATACGAGCGACGGGAAGATTTTGAAGCTCGGCTATTAACTCA GTTTCCAAATGCTGAGAAAATGAAGACGACATCTCCACCCGGCGATGACATTAAGACTTCTCCAGGCCAG TACATTCAGTGCTTCACAGTGAAGCCCAAGCTAGATTTGCCCCCTAAGTTTCACCGGCCAGTGTCTGAAC AGATTGTCAGTTTTTACAGGGTGAATGAGGTCCAGCGATTTGAATATTCACGGCCCATCCGGAAAGGAGA AAAAAACCCAGACAATGAATTTGCGAATATGTGGATCGAGAGAACCATCTACACCACAGCCTATAAACTG CCGGGAATTTTAAGGTGGTTTGAAGTCAAATCTGTCTTCATGGTAGAGATCAGTCCACTGGAGAATGCCA TCGAGACGATGCAGCTGACCAATGACAAGATTAGCAGCATGATCCAGCAGCACCTGGATGACCCAGGCCT GCCTATCAACCCTCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGAGGCTTTGCCAAT TATGAGAAGGCTTTCTTCACAGACCGATACCTGCAGGAGCATCCTGAGGCCCATGGGCAGATCGAGAAGC TCAAGGACCTGATAGCCTGGCAGATTCCATTTTTGGCTGAAGGGATCAGGATTCATGGAGACAAAGTCAC AGAGGCCCTGAGGCCATTCCATGAGCGCATGGAAGCCTGTTTCAAGCAGCTGAAGGAAAAAGTGGAAAAG CAATATGGCGTCAGGACCATGCCCTCAGGCTTGGATGACAGGAGAGGCAGTCGTCCTCGGTCCATGGTGC GGTCCTTCACAATGCCATCATCCTCTCGGCCACTGTCAGTGGCTTCTGTCTCTTCTTTCTCCTCTGACAG CACCCCTTCAAGGCCAGGCTCTGATGGATTTGCTCTCGAGCCTCTGCTGCCGAAGAAGATGCACTCCAGG TCCCAGGACAAGCTAGACAAGGACGATCCAGATAAAGAGAAGAAGGACAAGAAGAAGGAGAAAAGGAACA GCAAACACCAAGAGATATTTGATAAGGAATTCAAGCCTGCTGACAGCTCCCTGCAGCAGTCTGAGGCTGT GATCCTGTCTGAAACGATAAGTCCCCTGCGACCCCAAAGACCCAAGAGCCAGGTGATCAATGTCATTGGA AGCGAAAGACGCTTCTCTGTGTCTCCAGCTTCACCGTGCTCCCAGCCGACACCCCCTCCAGTCACACCAA GAGCCAAGCTCAGCTTCAGCATTCAGCCCAATCTGGAGCTGAATGGCATGATGGGGATGGATGTGGCCGA TGTCCCACCCCCTCTGCCCCTCAAAGGCAACATGGCAGATTATGGAAATCTGATGGAAAACCAGGATTTG ATGGGCTCACCCACATCTCCTCCTCCTCCCCCACAGCGGCAACAGCCACCTCCACTGCCCAGCAAAACAC CACCTCCTCCCCCTCCGAAGACGACCCGAAAGCAGACCTCAGTGGACTCTGGGATCGTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001143858
Insert Size	5595 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001143858.1, NP_001137330.1
RefSeq Size	6785 bp
RefSeq ORF	5595 bp
Locus ID	309081

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.