Myh6 (NM_017239) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Myhca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215216 representing NM_017239 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGATGCCCAGATGGCTGACTTCGGGGCAGCAGCCCCATACCTCCGCAAGTCAGAGAAGGAGCGCC TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACAGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT CAAGGCCAAGATCGTGTCCCGGGAAGGGGGCAAGGTCACTGCCGAAACTGAAAACGGCAAGACGGTGACT GTGAAGGAGGACCAGGTGATGCAGCAGAACCCTCCGAAATTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCATGAGCCAGCTGTGCTCTACAATCTCAAGGAGCGCTATGCGGCCTGGATGATCTATACCTA CTCAGGCCTCTTCTGTGTCACCGTCAACCCCTATAAGTGGCTGCCAGTGTACAATGCGGAAGTGGTAGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCTCCACCCCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACTGGAGAATCCGGAGCGGGGAAGACTGTCAA CACGAAGCGTGTCATCCAGTACTTTGCTAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGACAATCCT AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCTGCTCTGGAGGCCTTTGGCAACG CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAGTTCATCAGGATCCACTTTGGAGCCACAGG AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCTGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT GAGAGGAACTACCATATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG TTACCAACAACCCGTACGACTATGCCTTCGTCTCTCAGGGAGAGGTGTCTGTGGCCTCCATTGATGACTC CGAGGAGCTTTTGGCCACTGATAGCGCCTTTGATGTGCTGGGCTTCACAGCAGAGGAGAAGGCCGGTGTC TACAAGCTGACAGGCGCCATCATGCACTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CGGAGCCAGACGGCACAGAAGATGCTGACAAATCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCT CAAGGGTCTGTGTCACCCTCGGGTGAAGGTGGGTAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAG GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA TCAACGCAACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGTGTCCTGGACATCGCCGGCTTTGA GATCTTTGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTGACT TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGA GGAGTGCATGTTCCCCAAGGCCACAGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAG TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCTCTGGTCCACTATG CTGGCACCGTGGACTACAACATCTTGGGCTGGCTGGAGAAGAACAAGGACCCTCTCAACGAGACGGTGGT GGGGCTGTACCAGAAGTCCTCCCTCAAACTCATGGCCACACTCTTCTCCACCTACGCTTCTGCTGATACC GGTGACAGTGGGAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAGACAGTGTCTGCTCTCCACC GGGAAAATCTGAACAAGCTGATGACAAACCTGAGGACCACCCATCCTCACTTTGTGCGCTGCATCATCCC CAATGAGCGGAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAACGGAGTG CTGGAGGGTATCCGCATCTGTAGGAAGGGCTTCCCCAACCGCATTCTTTATGGGGACTTCCGGCAGAGGT ATCGAATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAGCT GCTGGGCTCCCTGGACATTGACCACAACCAGTACAAGTTTGGTCACACCAAGGTGTTCTTCAAGGCGGGG CTGCTGGGGCTGCTGGAGGAGATGCGAGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCTCAAG CCCGAGGCCAGCTCATGCGCATTGAGTTCAAGAAGATGGTGGAGCGCAGGGACGCCCTGCTGGTTATCCA GTGGAACATCCGCGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCG CTGCTGAAGAGCGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAGGAGTTCGGGCGAGTCAAAGATG CACTAGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA TGACCTGCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGATGCCGAGGAGCGCTGCGACCAGCTG ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA TGAACGCCGAGCTCACGGCCAAGAAGCGCAAGCTGGAAGACGAGTGCTCAGAGCTCAAGAAAGATATCGA TGACCTGGAGCTGACCCTGGCCAAGGTGGAGAAGGAAAAGCACGCAACAGAGAACAAGGTTAAAAACCTG ACAGAGGAGATGGCCGGGCTGGACGAGATCATTGCCAAGCTGACCAAGGAGAAGAAAGCTCTTCAAGAGG CCCACCAGCAAGCCCTAGATGACCTTCAGGCTGAGGAAGACAAGGTCAACACACTGATCAAGTCTAAAGT CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAGGTGCGCATGGACCTG GAGCGAGCAAAGCGGAAGCTGGAGGGTGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAACG ACAAGCTTCAGCTGGAGGAAAAGCTCAAGAAGAAAGAGTTTGACATCAGTCAGCAGAACAGTAAAATAGA GGATGAGCAGGCCCTGGCCCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG GAGGAGGAGCTAGAGGCCGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCAGACCTGACCCGGG AGCTGGAGGAGATCAGTGAGAGGCTAGAGGAAGCCGGTGGGGCCACATCTGTGCAGATAGAGATGAACAA GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCATGAGGCCACA GCCGCGGCCCTGCGCAAGAAGCACGCAGACAGCGTGGCCGAGCTGGGCGAGCAGATAGACAATCTACAGC GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAACTGGAGCTGGATGACGTCACCTCCAACAT GGAGCAGATCATCAAGGCCAAGGCAAACCTGGAGAAAGTGTCCCGGACACTGGAGGACCAGGCCAATGAA TACCGGGTGAAGTTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA CAGAGAACGGCGAGCTGGCTAGGCAACTGGAAGAAAAGGAGGCACTGATTTCCCAGCTGACCCGGGGCAA GCTCTCCTATACCCAGCAGATGGAGGACCTCAAGAGGCAGCTGGAGGAGGAAGGCAAGGCCAAGAATGCC TTGGCCCACGCACTGCAGTCAGCCCGGCATGACTGCGACCTGCTGCGGGAACAGTACGAAGAAGAAATGG AGGCCAAGGCCGAGCTGCAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA TGAGACGGACGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTTCAG GATGCTGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGCTCCTCTCTGGAGAAGACCAAGCACAGGCTGC AGAACGAGATCGAGGACCTGATGGTGGATGTGGAGCGCTCCAATGCGGCCGCCGCAGCCCTGGACAAAAA GCAGAGGAACTTCGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCCCAGTCAGAGCTGGAG TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAATGCCTATGAGGAGTCTC TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA GCTGGGAGAAGGGGGTAAAAATGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG GAACTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCATGAGGAGGGCAAGATCCTCCGAGCCC AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGACGAGGAGATGGAGCA GGCCAAGCGCAACCACCTGCGGGTGGTGGACTCCCTGCAGACCTCCCTGGATGCCGAGACGCGCAGCCGC AACGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATGGAGATCCAGCTCAGTCAGG CCAATAGAATAGCCTCAGAGGCCCAGAAGCACTTGAAGAACGCCCAAGCCCACTTGAAGGACACCCAGCT CCAGCTGGATGACGCAGTCCGTGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAAC ACCCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCCGTGGTGGAGCAGACAGAGCGGTCTCGGAAGCTGG CAGAGCAGGAGCTGATCGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCCCAGAACACCAGCCTCATCAA CCAGAAGAAGAAGATGGATGCAGACCTCTCCCAGCTCCAGACAGAGGTGGAGGAGGCGGTGCAGGAGTGT AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCCATGATGGCCGAGGAGCTGAAGAAGG AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACCTGCAGCA CCGGCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGTGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCGGAGTCGGTGAAGGGCATGAGGA AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTGGTGCGGCTGCA GGACCTGGTGGACAAGCTGCAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAAGAGGCGGAGGAACAG GCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCAGAGGAGAGGGCGGACA TTGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGTGACATTGGCGCCAAGCAGAAAATGCACGA TGAGGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017239
Insert Size	5820 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017239.2, NP_058935.2
RefSeq Size	5925 bp
RefSeq ORF	5820 bp
Locus ID	29556
Gene Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

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