Myh6 (NM_017239) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Myhca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215216 representing NM_017239 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGATGCCCAGATGGCTGACTTCGGGGCAGCAGCCCCATACCTCCGCAAGTCAGAGAAGGAGCGCC TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACAGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT CAAGGCCAAGATCGTGTCCCGGGAAGGGGGCAAGGTCACTGCCGAAACTGAAAACGGCAAGACGGTGACT GTGAAGGAGGACCAGGTGATGCAGCAGAACCCTCCGAAATTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCATGAGCCAGCTGTGCTCTACAATCTCAAGGAGCGCTATGCGGCCTGGATGATCTATACCTA CTCAGGCCTCTTCTGTGTCACCGTCAACCCCTATAAGTGGCTGCCAGTGTACAATGCGGAAGTGGTAGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCTCCACCCCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACTGGAGAATCCGGAGCGGGGAAGACTGTCAA CACGAAGCGTGTCATCCAGTACTTTGCTAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGACAATCCT AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCTGCTCTGGAGGCCTTTGGCAACG CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAGTTCATCAGGATCCACTTTGGAGCCACAGG AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCTGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT GAGAGGAACTACCATATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG TTACCAACAACCCGTACGACTATGCCTTCGTCTCTCAGGGAGAGGTGTCTGTGGCCTCCATTGATGACTC CGAGGAGCTTTTGGCCACTGATAGCGCCTTTGATGTGCTGGGCTTCACAGCAGAGGAGAAGGCCGGTGTC TACAAGCTGACAGGCGCCATCATGCACTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CGGAGCCAGACGGCACAGAAGATGCTGACAAATCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCT CAAGGGTCTGTGTCACCCTCGGGTGAAGGTGGGTAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAG GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA TCAACGCAACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGTGTCCTGGACATCGCCGGCTTTGA GATCTTTGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTGACT TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGA GGAGTGCATGTTCCCCAAGGCCACAGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAG TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCTCTGGTCCACTATG CTGGCACCGTGGACTACAACATCTTGGGCTGGCTGGAGAAGAACAAGGACCCTCTCAACGAGACGGTGGT GGGGCTGTACCAGAAGTCCTCCCTCAAACTCATGGCCACACTCTTCTCCACCTACGCTTCTGCTGATACC GGTGACAGTGGGAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAGACAGTGTCTGCTCTCCACC GGGAAAATCTGAACAAGCTGATGACAAACCTGAGGACCACCCATCCTCACTTTGTGCGCTGCATCATCCC CAATGAGCGGAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAACGGAGTG CTGGAGGGTATCCGCATCTGTAGGAAGGGCTTCCCCAACCGCATTCTTTATGGGGACTTCCGGCAGAGGT ATCGAATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAGCT GCTGGGCTCCCTGGACATTGACCACAACCAGTACAAGTTTGGTCACACCAAGGTGTTCTTCAAGGCGGGG CTGCTGGGGCTGCTGGAGGAGATGCGAGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCTCAAG CCCGAGGCCAGCTCATGCGCATTGAGTTCAAGAAGATGGTGGAGCGCAGGGACGCCCTGCTGGTTATCCA GTGGAACATCCGCGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCG CTGCTGAAGAGCGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAGGAGTTCGGGCGAGTCAAAGATG CACTAGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA TGACCTGCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGATGCCGAGGAGCGCTGCGACCAGCTG ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA TGAACGCCGAGCTCACGGCCAAGAAGCGCAAGCTGGAAGACGAGTGCTCAGAGCTCAAGAAAGATATCGA TGACCTGGAGCTGACCCTGGCCAAGGTGGAGAAGGAAAAGCACGCAACAGAGAACAAGGTTAAAAACCTG ACAGAGGAGATGGCCGGGCTGGACGAGATCATTGCCAAGCTGACCAAGGAGAAGAAAGCTCTTCAAGAGG CCCACCAGCAAGCCCTAGATGACCTTCAGGCTGAGGAAGACAAGGTCAACACACTGATCAAGTCTAAAGT CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAGGTGCGCATGGACCTG GAGCGAGCAAAGCGGAAGCTGGAGGGTGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAACG ACAAGCTTCAGCTGGAGGAAAAGCTCAAGAAGAAAGAGTTTGACATCAGTCAGCAGAACAGTAAAATAGA GGATGAGCAGGCCCTGGCCCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG GAGGAGGAGCTAGAGGCCGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCAGACCTGACCCGGG AGCTGGAGGAGATCAGTGAGAGGCTAGAGGAAGCCGGTGGGGCCACATCTGTGCAGATAGAGATGAACAA GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCATGAGGCCACA GCCGCGGCCCTGCGCAAGAAGCACGCAGACAGCGTGGCCGAGCTGGGCGAGCAGATAGACAATCTACAGC GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAACTGGAGCTGGATGACGTCACCTCCAACAT GGAGCAGATCATCAAGGCCAAGGCAAACCTGGAGAAAGTGTCCCGGACACTGGAGGACCAGGCCAATGAA TACCGGGTGAAGTTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA CAGAGAACGGCGAGCTGGCTAGGCAACTGGAAGAAAAGGAGGCACTGATTTCCCAGCTGACCCGGGGCAA GCTCTCCTATACCCAGCAGATGGAGGACCTCAAGAGGCAGCTGGAGGAGGAAGGCAAGGCCAAGAATGCC TTGGCCCACGCACTGCAGTCAGCCCGGCATGACTGCGACCTGCTGCGGGAACAGTACGAAGAAGAAATGG AGGCCAAGGCCGAGCTGCAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA TGAGACGGACGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTTCAG GATGCTGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGCTCCTCTCTGGAGAAGACCAAGCACAGGCTGC AGAACGAGATCGAGGACCTGATGGTGGATGTGGAGCGCTCCAATGCGGCCGCCGCAGCCCTGGACAAAAA GCAGAGGAACTTCGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCCCAGTCAGAGCTGGAG TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAATGCCTATGAGGAGTCTC TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA GCTGGGAGAAGGGGGTAAAAATGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG GAACTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCATGAGGAGGGCAAGATCCTCCGAGCCC AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGACGAGGAGATGGAGCA GGCCAAGCGCAACCACCTGCGGGTGGTGGACTCCCTGCAGACCTCCCTGGATGCCGAGACGCGCAGCCGC AACGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATGGAGATCCAGCTCAGTCAGG CCAATAGAATAGCCTCAGAGGCCCAGAAGCACTTGAAGAACGCCCAAGCCCACTTGAAGGACACCCAGCT CCAGCTGGATGACGCAGTCCGTGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAAC ACCCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCCGTGGTGGAGCAGACAGAGCGGTCTCGGAAGCTGG CAGAGCAGGAGCTGATCGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCCCAGAACACCAGCCTCATCAA CCAGAAGAAGAAGATGGATGCAGACCTCTCCCAGCTCCAGACAGAGGTGGAGGAGGCGGTGCAGGAGTGT AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCCATGATGGCCGAGGAGCTGAAGAAGG AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACCTGCAGCA CCGGCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGTGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCGGAGTCGGTGAAGGGCATGAGGA AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTGGTGCGGCTGCA GGACCTGGTGGACAAGCTGCAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAAGAGGCGGAGGAACAG GCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCAGAGGAGAGGGCGGACA TTGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGTGACATTGGCGCCAAGCAGAAAATGCACGA TGAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215216 representing NM_017239 Red=Cloning site Green=Tags(s) MTDAQMADFGAAAPYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKIVSREGGKVTAETENGKTVT VKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKDNP NANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKA ERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLGFTAEEKAGV YKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQ VYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGK SNNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADT GDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGV LEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAG LLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKMVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKP LLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQL IKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNL TEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLIKSKVKLEQQVDDLEGSLEQEKKVRMDL ERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEEL EEELEAERTARAKVEKLRSDLTRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANE YRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNA LAHALQSARHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQ DAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELE SSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKL ELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSR NEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNAQAHLKDTQLQLDDAVRANDDLKENIAIVERRN TLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQEC RNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEAR VRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLVRLQDLVDKLQLKVKAYKRQAEEAEEQ ANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017239
ORF Size	5817 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017239.2, NP_058935.2
RefSeq Size	5925 bp
RefSeq ORF	5820 bp
Locus ID	29556
MW	223.5 kDa
Gene Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

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