Nefm (NM_017029) Rat Untagged Clone

CAT#: RN215467

Nefm (untagged ORF) - Rat neurofilament, medium polypeptide (Nefm), (10 ug)



  "NM_017029" in other vectors (3)

CNY 10,830.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
Chicken Anti-Neurofilament NF-M ck Antibody
    • 100 ul

CNY 5,049.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Nef3; Nfm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215467 representing NM_017029
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGCGTCACCGAGACCCCGTCCAGCT
TCAGTCGTGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC
CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGCCTCGCCTACAGCTCGGCTATGCTCAGCTCG
GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCTTCGCTGCTTAACGGCGGCTCCGGCGGCGACTACAAGC
TGTCCCGCTCAAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGTTTCGCCGGCTACATCGAGAAAGT
GCACTACTTGGAACAACAGAACAAGGAGATCGAGGCAGAGATCCACGCGCTGCGGCAGAAGCAGGCCTCG
CACGCCCAGCTGGGTGACGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTGGAGATGGTGAATC
ACGAGAAGGCTCAAGTGCAGCTGGACTCTGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT
CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATCCGGGCGCTGCGCAAAGACATAGAGGAG
TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCCTTCCTGCGGA
GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCCCAGATCCAGGCGTCGCACATCACCGTAGAGCGCAA
AGACTACCTGAAGACAGACATCTCCACGGCGCTGAAAGAGATCCGCTCCCAGCTCGAGTGTCACTCCGAC
CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA
ACAAGGAGGCCATCCGCTCCGCTAAAGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT
TGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAACGGCAGCTCAGCGACATCGAGGAGCGCCAC
AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGCTGGAAAATGAGCTTCGGGGAACAAAGTGGG
AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCTCTGGACATCGAGATCGC
CGCATATAGGAAACTACTGGAGGGTGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACTGGGCCT
CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA
AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATTGAGGAGACTAAAGTGGAAGATGAGAAGTCAGA
AATGGAAGACGCCCTCACAGTCATTGCAGAGGAATTGGCAGCCTCTGCCAAAGAGGAGAAAGAAGAGGCA
GAAGAAAAGGAAGAGGAACCGGAAGTTGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG
AAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAAGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTCAGA
CCAGGCAGAAGAGGGAGGATCTGAGAAGGAAGGCTCGAGTGAAAAGGATGAAGGTGAGCAAGAAGAAGAA
GGGGAAACTGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAACAGAGGGAAAGG
TCGAGGAAATGGCTATCAAGGAGGAAATCAAGGTCGAGAAGCCCGAGAAAGCCAAGTCCCCTGTGCCAAA
ATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAAGCCAAAGCCGGAAAGGATGAGCAGAAGGAGGAAGAG
AAAGTTGAGGAGAAGAAGGAGGTAGCCAAGGAATCACCCAAGGAAGAGAAGGTGGAGAAAAAGGAGGAGA
AGCCAAAAGATGTCCCAGATAAAAAGAAGGCTGAGTCCCCAGTGAAAGAAAAGGCCGTAGAGGAAATGAT
CACCATTACTAAGTCGGTAAAGGTGAGCCTGGAGAAAGACACCAAAGAGGAGAAGCCTCAGCAGCAGGAG
AAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGTAGTGAGGAGGAAGTGGGTGACAAAAGCCCGCAAGAAT
CCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGAAACTCAGGA
GAAGGGCAGTGGGCAAGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCTGCGGAGGAA
AAGAAAGGGGAGGATAGAAGTGATGACAAAGTGGTGGTGACCAAGAAGGTAGAAAAAATCACCAGCGAGG
GAGGCGATGGTGCTACCAAATACATCACCAAATCTGTTACTGTCACTCAAAAGGTTGAAGAGCATGAGGA
GACCTTTGAGGAGAAGCTGGTGTCAACTAAAAAGGTAGAAAAGGTCACTTCACATGCCATAGTCAAGGAA
GTCACCCAGGGTGACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_017029
Insert Size 2538 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017029.2, NP_058725.1
RefSeq Size 3067 bp
RefSeq ORF 2538 bp
Locus ID 24588
UniProt ID P12839
Gene Summary neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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