Jund (NM_001286966) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216269 representing NM_001286966 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGAAAGACGCGCTGACGCTCAGCCTGGCGGAGCAGGGAGCGGCGGGATTGAAACCAGGGTCGG CCACTGCACCTTCTGCATTGCGCCCCGACGGCGCCCCCGACGGGCTGCTGGCTTCGCCGGATCTTGGGCT GCTCAAACTCGCGTCGCCGGAGCTGGAAAGGCTGATCATCCAGTCCAACGGGCTGGTGACCACCACCCCG ACCAGTACGCAGTTCCTCTACCCTAAGGTGGCGGCCAGCGAGGAGCAGGAGTTCGCCGAAGGCTTCGTCA AGGCGCTGGAGGACCTGCACAAGCAGAGCCAGCTGGGTGCGGCCACCGCGGCCACCTCAGGGGCTCCCGC GCCCCCCGCGCCCGCGGACCTGGCAGCCACCCCCGGGGCCACGGAGACCCCGGTCTACGCCAACCTGAGC AGTTTCGCGGGTGGCGCCGGGCCCCCTGGGGGCGCGGCCACAGTGGCCTTCGCCGCGGAGCCAGTGCCCT TCCCGCCGCCCCCGGGCGCGCTGGGGCCGCCGCCACCTCCGCATCCACCGCGTCTGGCCGCGCTCAAGGA CGAGCCGCAGACCGTGCCGGACGTGCCGAGCTTCGGCGACAGCCCTCCACTGTCGCCCATCGACATGGAC ACGCAGGAACGCATCAAGGCGGAGCGCAAGAGGCTGCGCAACCGCATCGCCGCTTCCAAATGCCGCAAGC GCAAGCTGGAGCGTATCTCGCGCCTGGAGGAGAAAGTCAAGACCCTCAAAAGCCAGAACACCGAGCTGGC GTCCACCGCGAGCCTGCTGCGCGAGCAGGTGGCGCAGCTCAAACAGAAAGTCCTCAGCCACGTCAACAGC GGCTGCCAGCTGCTGCCCCAGCACCAGGTCCCCGCGTATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286966
Insert Size	882 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286966.1, NP_001273895.1
RefSeq Size	1703 bp
RefSeq ORF	882 bp
Locus ID	24518
UniProt ID	P52909
Gene Summary	The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013] Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (JunD-FL) is derived from an upstream AUG (S1 ORF, PMID:12105216), while the shorter isoform (deltaJunD) is derived from a downstream AUG (S3 ORF, PMID:12105216). This RefSeq represents the shorter isoform (deltaJunD).

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