Htra3 (NM_001271027) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1308120
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216716 representing NM_001271027 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCGCGCGCCCTACTCCCTGCCACGCTGGCCACGCTGGCCACGCTAGCTGTGTCGGTCCTGGCCC GGGAGCCCCCGGCGGCGCCTTGTCCTGCGCGCTGCGACGTGTCGCGCTGTCCGAGCCCTCGCTGCCCTGG GGGCTATGTGCCAGACCTCTGCAACTGCTGCTTGGTGTGCGCCGCCAGCGAGGGTGAGCCCTGCGGCCGC CCCCTGGACTCGCCATGCGGGGACAGTCTGGAGTGCGTACGCGGCGTGTGCCGCTGCCGTTGGACCCACA CGGTGTGTGGCACAGACGGGCACACATATGCCGACGTGTGCGCGCTGCAGGCCGCCAGCCGTCGTGCGTT GCAGATCTCCGGGACTCCAGTGCGCCAGCTGCAGAAGGGTGCCTGCCCCTCTGGTCTCCATCAGCTGACC AGTCCGCGGTACAAGTTCAACTTCATCGCCGATGTGGTGGAGAAGATCGCGCCAGCCGTGGTCCATATAG AGCTCTTTCTGAGGCACCCTCTGTTTGGCCGAAATGTGCCACTGTCCAGTGGCTCGGGCTTCATCATGTC AGAAGCCGGCTTGATCGTCACCAACGCCCACGTGGTCTCCAGCTCCAACACCGCCTCAGGTCGGCAGCAG CTGAAGGTGCAACTGCAGAATGGGGATGCTTATGAGGCCACCATCCAGGACATCGACAAGAAGTCAGACA TCGCCACCATCTTAATCCACCCCAACAAAAAGCTCCCCGTGTTACTGCTGGGTCACTCGGCAGACCTGCG GCCTGGCGAGTTTGTGGTGGCCATCGGCAGTCCCTTTGCCCTGCAAAACACCGTGACAACGGGCATCGTC AGCACTGCCCAGCGGGATGGCAAAGAGCTGGGCCTCCGGGATTCGGACATGGACTATATCCAGACAGACG CCATCATCAATTATGGGAACTCAGGAGGCCCCCTGGTGAACCTGGATGGTGAGGTCATCGGCATCAACAC GCTCAAGGTGGCGGCTGGTATCTCCTTTGCCATCCCCTCGGATCGCATCACGCGCTTCCTCTCTGAGTTC CAAGACAAGCATGTGAAAGACTGGAAGAAGCGCTTCATTGGCATCCGGATGCGGACCATCACGCCAAGTT TGGTGGAGGAACTGAAGACTGCCAACCCAGATTTTCCAGCAGTGAGCAGTGGAATATATGTTCAAGAGGT CGTTCCCAACTCACCTTCTCAGAGAGGAGGCATCCAAGATGGCGACATCATAGTCAAAGTCAATGGACGT CCCCTGGTAGATTCCAGTGAGCTGCAGGAGGCGGTCCTGAATGAGTCCTCACTCCTGCTGGAGGTGCGGC GGGGGAATGATGACCTGCTCTTCAGCATCATGCCGGAGGTGGTGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271027
Insert Size	1380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271027.1, NP_001257956.1
RefSeq Size	2440 bp
RefSeq ORF	1380 bp
Locus ID	360959
UniProt ID	D3ZA76
Gene Summary	Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).[UniProtKB/Swiss-Prot Function]

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