Vcan (NM_001170559) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217058 representing NM_001170559 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGACCTGAT CTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTATCCTACTGAGACTTCCTATGTGTGCACCT GTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTGATGAATGTCACTCTAACCCTTGTCGGAA TGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCTCTGCCTTCCGAGTTATGTCGGTGCACTC TGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAATTCCAAGGGCAATGCTACAAGTACTTTG CTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCCTGCAGGGTGCCCACCTCACAAGCATCCT TTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGATTACCAGTGGATTGGCCTCAATGACAAG ATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTGCAATATGAGAACTGGAGACCCAACCAGC CAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCATTTGGCATGAGAATGGCCAGTGGAATGA CGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGGAACAGTTGCTTGCGGCCAACCCCCTGTT GTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTATGAAATCAACTCCTTGATTAGATACCACT GCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGTGCCTAGGAAATGGGAGATGGGCAATGCC TAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTATTCTAAGAAATACTTAAAAAATTCCTCA TCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCATCGCTGGAGCCGGAGGTGGCAGGAAACGA GGCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170559
Insert Size	1968 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170559.1, NP_001164030.1
RefSeq Size	4175 bp
RefSeq ORF	1968 bp
Locus ID	114122
UniProt ID	Q9ERB4
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006] Transcript Variant: This variant (4) lacks two alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform 4, also referred to as V3), compared to isoform 1.

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