Tgfbi (NM_053802) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Big-h3; BIGH3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217102 representing NM_053802 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTCCTCGTGCGGCTGCTGCCCCTCGCTCTGGCTCTGTCTCTGGGCTTCACCGGGACCCTGGCGG GCCCGGCCAAGTCACCCTATCAGCTGGTGCTGCAGCACAGCCGGCTCCGGGGCCGCCAGCACGGCCCCAA TGTATGTGCTGTGCAGAAGGTCATTGGCACTAACAAGAAATACTTCACCAACTGCAAGCAGTGGTACCAG AGGAAGATCTGCGGCAAGTCGACAGTCATCAGCTATGAGTGCTGCCCCGGATATGAAAAGGTCCCAGGAG AGAAAGGCTGCCCTGCAGCTCTTCCGCTCTCCAATCTGTACGAGACCTTGGGAATTGTGGGATCGACCAC CACACAGCTGTACACAGATCGCACAGAAAAGCTGAGGCCTGAGATGGAGGGACCCGGCAGCTTCACCATC TTTGCCCCTAGCAACGAGGCCTGGTCCTCCTTGCCTGCGGAAGTGCTGGACTCCCTGGTGAGCAATGTCA ACATTGAACTGCTCAACGCCCTCCGCTACCACATGGTGGACAGACGGGTCCTGACAGACGAGCTTAAGCA CGGCATGGCTCTCACCTCCATGTACCAGAACTCCAACATCCAGATCCATCACTATCCCAATGGGATTGTA ACTGTTAACTGTGCCCGGCTGCTGAAAGCTGACCACCATGCGACCAACGGCGTGGTGCATCTCATTGATA AGGTCATTTCCACCGTTACCAACAACATCCAGCAGATCATCGAGATTGAGGACACCTTTGAGACACTTCG GGCCGCCGTGGCTGCATCAGGACTCAATACCGTGCTGGAGGGTGACGGCCAGTTCACACTCTTGGCCCCA ACCAACGAGGCCTTTGAGAAGATTCCTGCTGAGACCTTGAACCGGATCCTGGGTGACCCCGAGGCCCTGA GAGACCTGCTAAACAATCATATCCTGAAGACAGCCATGTGTGCTGAGGCCATTGTGGCTGGAATGGCCAT GGAGACCCTGGGGGGCACCACACTGGAGGTGGGCTGCAGTGGAGACATGCTCACTATTAACGGGAAGGCT GTCATCTCCAACAAAGACATCCTGGCCACCAACGGTGTCATCCACTTCATTGATGAGCTGCTCATCCCAG ATTCAGCCAAGACATTGCTCGAGCTCGCTTCGGAATCTGACGTCTCCACAGCCATTGACCTCCTCAGACA AGCTGGCCTCAGCACGCATCTCTCTGGAAAAGAACAGTTGACCTTCCTGGCCCCTCTGAATTCTGTGTTC AAAGATGGTGTCCCCCGCATTGACGGCCAAATGAAGACTTTGCTTCTGAACCACATGGTCAAAGGGCAGT TGGCCTCCAAATATTTGTACTCCGGACAGACACTGGACACACTGGGTGGCAAAAAGCTTCGAGTTTTTGT TTATCGAAATAGCCTCTGCATTGAAAACAGCTGCATTGCCGCCCATGACAAGAAGGGACGGTATGGGACC CTGTTCACCATGGACCGGATGCTGACACCCCCTATGGGGACTGTTATGGATGTCCTGAAGGGGGACAACC GTTTTAGCATGCTGGTGGCCGCCATCCAGTCTGCAGGCCTGATGGAGACCCTCAACCGGGAAGGGGTCTA CACTGTTTTCGCTCCCACGAACGAAGCGTTCCAAGCCATGCCTCCAGAAGAGCTGAACAAACTCTTGGCA AATGCCAAGGAACTTACCAACATCCTGAAGTACCACATTGGGGATGAGATCCTGGTTAGCGGAGGCATTG GGGCTCTGGTGCGGCTGAAGTCTCTCCAAGGGGACAAACTGGAAGTCAGCTCGAAAAACAATGTGGTGAG TGTCAATAAGGAACCTGTTGCCGAAACCGACATCATGGCCACAAACGGTGTGGTGTATGCCATCAACACT GTTCTGCAACCGCCAGCCAACCGGCCACAAGAACGGGGAGAAGAGTTGGCAGACTCTGCCCTTGAAATCT TCAAGAAGGCGTCAGCCTATTCCAGGAGTGCCCAGAGTTCTATGCGACTTGCCCCTGTCTATCAGCGGTT ACTAGAGAGGATGAAGCGGAGAGGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053802
Insert Size	2058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053802.1, NP_446254.1
RefSeq Size	2696 bp
RefSeq ORF	2058 bp
Locus ID	116487
Gene Summary	mouse homolog is an extracellular matrix protein; human homolog is associated with hereditary corneal dystrophies [RGD, Feb 2006]

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