Sh3bp1 (NM_001171981) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217107 representing NM_001171981 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGAGGCAGCTGCACCGCATGCGGCAGCTGGCCCACACGGGCAGCTCGGGACGCACCCCTGAGA CTGCCGAGTTCTTGGGTGAGGACCTGCTGCAGGTAGAGCAGCGGTTGGAACCAGCTAAGCGAGCAGCCCA CAATGTTCACAAAAGGCTGCAGGCCTGTCTGCAGGGCCAAAGCGGGGCTGACATGGACAAGCGGGTGAAG AAGCTTCCCCTCATGGCTCTTTCTACCGCAATGGCTGAAAGCTTCAAGGAGTTGGATCCCGATTCCAGCA TGGGGAAAGCCTTAGAGATGAGCTGTGCCATCCAGAACCAGCTGGCCAGAATCCTAGCAGAGTTTGAGAT GACCCTGGAGAGAGATGTCCTGCAGCCGCTCAACAGGCTGAGCGAGGAGGAGCTGCCTGCCATCCTCAAG CGCAAGAAGAGCCTTCAGAAGCTGGTGTCTGACTGGAACACCCTCAAAAGCAGGCTCAGCCAGGCAGCCA AGAACTCGGGAAGCAGCCAAAGCCTGGGTGGTGGCTCAAGCAGTCACACCCACATGGCCACTGCCAACAA GGTGGAGACGCTGAAGGAAGACGAGGAGGAGCTGAAGAGAAAGGTGGAACAGTGCAAGGATGAGTACCTG GCTGATCTCTACCACTTCTCCACCAAGGAGGACTCATATGCCAACTACTTTACTCATCTCCTGGAGATTC AGGCTGACTACCATCGCAAGTCACTAACCTCCCTTGACACGGCCCTAGCTGAGCTGAGGGACAACCACAG CCAAGCAGACTCCTCCCCCTTGACGACGGCTGCCCCCTTCTCCAGAGTATATGGGGTGTCCCTGAGAACC CACCTGCAAGATCTAGGCCGTGACATTGCCCTGCCCATCGAGGCTTGTGTCCTGTTGCTGCTGTCGGAGG GCATGCAGGAGGAGGGCCTCTTCCGTCTGGCTGCTGGGGCCTCTGTGCTGAAGCGCCTTAAGCAGACCAT GGCCTCAGATCCCCACAGCCTGGAGGAATTCTGCTCGGACCCCCATGCTGTAGCAGGTGCCCTCAAGTCC TATCTCCGGGAGCTGCCAGAGCCCCTGATGACCTCTGACCTCTATGATGACTGGATGAGAGCAGCCAGCC TGAAGGAACCAGGAGCCCGCCTGGAGGCCCTTCATGATGTTTGCAGCCGTCTACCCCAGGAGAACTTCAA CAACCTCCGGTACCTGATGAAGTTCTTGGCACTGCTGGCCGAGGAGCAGGACGTGAACAAGATGACACCC AGCAACATCGCCATTGTCTTGGGGCCCAACCTGCTGTGGCCTCCTGAGAAAGAAGGGGACCAGGCTCAAC TGGACGCTGCCTCTGTGTCCTCCATTCAGGTGGTGGGTGTTGTCGAGGTGCTGATACAGAATGCAGACAC CCTCTTTCCTGGAGATATCAACTTCAGTGTATCAGGCATCTTCTCAGGCCTGGCCCCCCAGGAGAAGCCC AATAGTCAACAGGTCTCAGAGGAATTGGCACCTGTTGCTGTGCCTGCCACAGCCGCTACCCCAACTCCCA CCCCAGCTCCTACCCCAGCTCCAGCCTCCATGACAGTAAAAGAAAGGACAGAGTCTGAATTGCCCAAACC AGCTTCCCCCAAGGTCAGCAGGAGCCCCACAGATACAACTGCTTTGGCAGAGGACATGACTCGGAAGACC AAGCGCCCAGCACCAGCCCGGCCTACCATGCCACCTCCCCAGCCTTCCAGCTCACGCTCATCCCCTCCAG CTCTATCCCTGCCCGCTGGCTCGGTCAGCCCTGGCACTCCCCAAGCTTTGCCACGCCGTCTGGTGGGCAC CAGCCTCCGGGCCCCCACCGTACCTCCCCCATTACCCCCTGCTCCTCCACAACCTGCCCGGCGACAGAGC CGGCGTTTACCAGTCTCCCCCTGCCCAGCCTCCCCTGTCATTTCAAACATGCCTGCTCAGGTGGACCAGG GAGCGGCTACAGAGGACAGAGGAGGCCCTGAGGCTGTGGGTGGGCATCCCCCTACCCCAGTTCTGCCACC CCAGCCCCGGCCCAGGGGCCTCATTTCAGAGACAGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001171981
Insert Size	2070 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001171981.1, NP_001165452.1
RefSeq Size	2407 bp
RefSeq ORF	2070 bp
Locus ID	300067
UniProt ID	D3ZFJ3
Gene Summary	GTPase activating protein/GAP which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form. By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration (PubMed:21658605). Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions. Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment. It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (By similarity).[UniProtKB/Swiss-Prot Function]

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