Trpv2 (NM_001270797) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Vrl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217179 representing NM_001270797 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCAGCCTCCAGCCCCCCAGCTTTCAGGCTGGAGACTTCCGATGGAGATGAAGAGGGCAATGCTG AGGTGAACAAGGGGAAGCAGGAACCGCCCCCCATGGAGTCACCATTCCAGAGGGAGGACCGGAATTCCTC CCCTCAGATCAAAGTGAACCTCAACTTCATAAAGAGACCTCCTAAAAACACTTCTGCTCCCAGCCAGCAG GAGCCAGATCGGTTTGACCGTGACCGACTCTTCAGTGTGGTCTCCCGGGGTGTCCCCGAGGAACTGACTG GACTGCTAGAATACCTGCGCTGGAACAGCAAGTACCTCACTGACTCTGCATACACAGAAGGCTCCACTGG AAAGACGTGCCTGATGAAGGCTGTGCTGAACCTTCAGGATGGGGTCAATGCCTGCATCATGCCGCTGCTG CAGATTGACAAGGATTCCGGCAATCCCAAGCCCCTCGTCAATGCCCAGTGCATCGATGAGTTCTACCAAG GCCACAGTGCGCTGCACATCGCCATAGAGAAGAGGAGCCTGCAGTGCGTGAAGCTGCTGGTAGAGAATGG AGCGGATGTTCACCTCCGAGCCTGTGGCCGCTTCTTCCAAAAGCACCAAGGAACTTGTTTCTATTTTGGA GAGCTACCTCTTTCTCTGGCTGCGTGCACCAAGCAGTGGGATGTGGTGACCTACCTCCTGGAGAACCCAC ACCAGCCGGCCAGCCTGGAGGCCACCGACTCCCTGGGCAACACAGTCCTGCATGCTCTGGTAATGATTGC AGATAACTCGCCTGAGAACAGTGCCCTGGTGATCCACATGTACGACGGGCTTCTACAAATGGGGGCGCGC CTCTGCCCCACTGTGCAGCTTGAGGAAATCTCCAACCACCAAGGCCTCACACCCCTGAAACTAGCCGCCA AGGAAGGCAAAATCGAGATTTTCAGGCACATTCTGCAGCGGGAATTCTCAGGACCGTACCAGCCCCTTTC CCGAAAGTTTACTGAGTGGTGTTACGGTCCTGTGCGGGTATCGCTGTACGACCTGTCCTCTGTGGACAGC TGGGAAAAGAACTCGGTGCTGGAGATCATCGCTTTTCATTGCAAGAGCCCGAACCGGCACCGCATGGTGG TTTTAGAACCACTGAACAAGCTTCTGCAGGAGAAATGGGATCGGCTCGTCTCAAGATTCTTCTTCAACTT CGCCTGCTACTTGGTCTACATGTTCATCTTCACCGTCGTTGCCTACCACCAGCCTTCCCTGGATCAGCCA GCCATCCCCTCATCAAAAGCGACTTTTGGGGAATCCATGCTGCTGCTGGGCCACATTCTGATCCTGCTTG GGGGTATTTACCTCTTACTGGGCCAGCTGTGGTACTTTTGGCGGCGGCGCCTGTTCATCTGGATCTCATT CATGGACAGCTACTTTGAAATCCTCTTTCTCCTTCAGGCTCTGCTCACAGTGCTGTCCCAGGTGCTGCGC TTCATGGAGACTGAATGGTACCTACCCCTGCTAGTGTTATCCCTAGTGCTGGGCTGGCTGAACCTGCTTT ACTACACACGGGGCTTTCAGCACACAGGCATCTACAGTGTCATGATCCAGAAGGTCATCCTTCGAGACCT GCTCCGTTTCCTGCTGGTCTACCTGGTCTTCCTTTTCGGCTTTGCTGTAGCCCTAGTAAGCTTGAGCAGA GAGGCCCGAAGTCCCAAAGCCCCTGAAGATAACAACTCCACAGTGACGGAACAGCCCACGGTGGGCCAGG AGGAGGAGCCAGCTCCATATCGGAGCATTCTGGATGCCTCCCTAGAGCTGTTCAAGTTCACCATTGGTAT GGGGGAGCTGGCTTTCCAGGAACAGCTGCGTTTTCGTGGGGTGGTCCTGCTGTTGCTGTTGGCCTACGTC CTTCTCACCTACGTCCTGCTGCTCAACATGCTCATTGCTCTCATGAGCGAAACTGTCAACCACGTTGCTG ACAACAGCTGGAGCATCTGGAAGTTGCAGAAAGCCATCTCTGTCTTGGAGATGGAGAATGGTTACTGGTG GTGCCGGAGGAAGAAACATCGTGAAGGGAGGCTGCTGAAAGTCGGCACCAGGGGGGATGGTACCCCTGAT GAGCGCTGGTGCTTCAGGGTGGAGGAAGTAAATTGGGTTGCTTGGGAGAAGACTCTTCCCACCTTATCTG AGGATCCATCAGGGCCAGGCATCACTGGTAATAAAAAGAACCCAACCTCTAAACCGGGGAAGAACAGTGC CTCAGAGGAAGACCATCTGCCCCTTCAGGTCCTCCAGTCCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270797
Insert Size	2286 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270797.1, NP_001257726.1
RefSeq Size	2811 bp
RefSeq ORF	2286 bp
Locus ID	29465
UniProt ID	Q9WUD2
Gene Summary	ion channel that may be activated by high temperature; may transduce high threshold heat responses in sensory ganglia [RGD, Feb 2006] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.

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