Gprc6a (NM_001271106) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217339 representing NM_001271106 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTATCATTTGTATTTATTACTTGCTTCATGATTCTTCTTGATACCTCCCAGTCTTGTCATACCC CAGATGACTTCGTGGCTATCACTTCTCCTGGACATATCATGATTGGTGGTTTGTTTGCCATTCACGAAAA AATGTTGTCCTCAGATGACCATCCCAGGCAACCACAAATCCAGAAGTGTGTTGGTTTTGAAATATCAGTG TTTCTTCAAACTCTGGCTATGATACACAGCATTGAGATGATCAATAACTCAAGCCTGTTGTCCGGAGTTA AGCTGGGGTATGAAATCTATGACACTTGTACTGAAGTCACAGCGGCAATGGCTGCCACCCTGAGGTTCCT CTCTAAATTCAACTGCTCTAGAGAAACCGTGATCTTTCAATGTGACTATTCCAGCTACGTGCCAAGGGTC AAGGCCATCATAGGTGCTGGCTACTCTGAAATATCCATGGCGGTCTCGAGGATGCTGAATTTACAGCTCA TGCCACAGGTGAGTTATGAATCCACTGCAGAAATCCTGAGTGACAAAATCCGCTTTCCTTCGTTTTTACG AACCGTGCCCAGTGACTTCTACCAAACTAAAGCAATGGCCCACCTGATCCGACAATCCGGATGGAACTGG GTTGGTGCCATAACAACCGACGATGATTATGGAAGACTAGCGCTCAACACATTTGCAATCCAGGCTGCTG AAAACAATGTGTGCATCGCCTTCAAAGAGGTTCTGCCAGCCTTCCTCTCAGATAATACCATTGAAGTGAG AATCAACCAGACTCTGGAGAAAATCATTGCCGAAGCCCAGGTCAATGTCATCGTGGTGTTTCTTAGGAAA TTCCACGTCTTCAATCTCTTCAACAAAGCCATCGAGAGGAAAATAAGTAAGATCTGGATTGCTAGTGATA ACTGGTCAACTGCTGCCAAGATTATCACCATTCCCAATGTTAAGAAGCTTGGCAAAGTAGTGGGCTTTAC TTTTAGGAGAGGAAATATGTCTTCTTTCCATTCTTTTCTTCAAACTCTGCATATGTACCCCAGTGACAAT AACAAACCCCTACATGAGTTTGCCATGCTTTTTTCTGCCTGTAAACACATCAAAGATGGTGATTTGAGCC AATGCATTTCCAACTATTCTCAGGCAACTTGGACCTATGACACTACCAAGACCATTGAGACCCATTTATT CAAGAGAAATGACTTCCTTTGGCATTATACTGAGCCAGGACTCATTCACAGCATTCAGCTTGCTGTGCTT GCCTTGGGCCATGCCATCCGGGATCTGTGCCAAGATCGAGACTGCCAGAAACCCAACGCCTTTCAGCCAT GGGAGCTACTTGCGGTGCTGAAAAACGTGACATTCACTGACGGAAAAAACTCATTTCATTTTGATGCCCA TGGGGATTTAAATACTGGTTACGAGGTGGTGCTCTGGAAAGAGACTAACGGCCTCATGACTGTCACGAAG ATGGCAGAATATGACCTGCAGCATGACGTCTTCATCACCACAAACCAAGAAACAAAGCATGAATTCAGGA AACTTAAGCAAATTCTATCTAAATGCTCCAAAGAATGCATTCCTGGTCAAATGAAGAAAGCCACAGGAAG CCAACACAGCTGTTGCTATGAATGTGTGAACTGCCCAGAAAACCACTACAGTAATGAGACAGACATGGAT CACTGCCTCGTATGCAACAACGAAACCCACTGGGCCCCAGTAAGGAGCACGATGTGCTTCGAGAAGGAAG TGGAGTATCTTGACTGGGATGACTCCTTGGCTCTCCTCCTCATTGCCCTCTCCCTACTTGGAATTGCCTT TGTTCTGGCCGTTGGCATAATATTTACAAGAAACCTGAAGACACCTGTGGTGAAATCATCTGGGGGATTA GTGGTCTGTTACGTGATGCTCGCCTGCCATGCCCTCAACTTTGCCAGCACAGGCTTTTTTATTGGAGAAC CACAGGACTTCACGTGCAAGACCAGGCAGACCCTGTTTGGCGTGAGCTTTACTCTGTGTGTCTCCTGTAT TTTGACGAAGTCCCTGAAAATTTTGCTAGCCTTCAGCTTCGACCCCACGCTGAAGACATTCCTGAAGTGT CTCTATAGGCCAGTCCCCATTGTCCTTACCTGCACGGGCATTCAAGTGGTCATTTGCACGCTTTGGCTGG TCCTGGCAGCACCTACTGTGGAAGAGAACACCTCCTTGCCTAGAGTCATTATCCTAGAATGTGAGGAGGG ATCTGCACTGGCGTTTGGCACCATGCTGGGCTACATTGCAGTTCTGGCCTTCATTTGTTTTGTATTTGCA TTCAAGGGGAGGAAGCTTCCTGAGAATTACAACGAAGCTAAGTTCCTGACCTTTGGGATGCTGATTTACT TCATAGCTTGGATCACATTCATCCCAGTCTATGCTACCACTTTCGGCAAGTATTTGCCCGCTGTGGAGAT CATAGTCATTCTGATATCCAACTATGGTATCCTCTGCTGTACATTCTTCCCCAAGTGCTACATTATTCTT TGCAAGCAAAAGACTAACACTAAGTCAGTCTTCCTCCAGATGGTTTACAATTACTCTGCTCACAGCGTGG ACAGCCTTGCCTTGAGTCACGTATCCCTGGACTCCGCCAGCCACAGTACTGCAACAACCAACCCGAGGCC TGGTAACAAGACTGCAGCCTGTCAGAATTACAAACATCTTCCTGTACAAGTGCTTGCTCACACAGGCATG GAAAAGACTATGCACGCGTCTAAAACTTTGCATCAGAAAAGAAGTTCAAGTATATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII
ACCN	NM_001271106
Insert Size	2787 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271106.1, NP_001258035.1
RefSeq Size	3033 bp
RefSeq ORF	2787 bp
Locus ID	294394
UniProt ID	Q70VB1
Gene Summary	Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids (By similarity).[UniProtKB/Swiss-Prot Function]

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