Gprc6a (NM_001271106) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217339 representing NM_001271106 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTATCATTTGTATTTATTACTTGCTTCATGATTCTTCTTGATACCTCCCAGTCTTGTCATACCC CAGATGACTTCGTGGCTATCACTTCTCCTGGACATATCATGATTGGTGGTTTGTTTGCCATTCACGAAAA AATGTTGTCCTCAGATGACCATCCCAGGCAACCACAAATCCAGAAGTGTGTTGGTTTTGAAATATCAGTG TTTCTTCAAACTCTGGCTATGATACACAGCATTGAGATGATCAATAACTCAAGCCTGTTGTCCGGAGTTA AGCTGGGGTATGAAATCTATGACACTTGTACTGAAGTCACAGCGGCAATGGCTGCCACCCTGAGGTTCCT CTCTAAATTCAACTGCTCTAGAGAAACCGTGATCTTTCAATGTGACTATTCCAGCTACGTGCCAAGGGTC AAGGCCATCATAGGTGCTGGCTACTCTGAAATATCCATGGCGGTCTCGAGGATGCTGAATTTACAGCTCA TGCCACAGGTGAGTTATGAATCCACTGCAGAAATCCTGAGTGACAAAATCCGCTTTCCTTCGTTTTTACG AACCGTGCCCAGTGACTTCTACCAAACTAAAGCAATGGCCCACCTGATCCGACAATCCGGATGGAACTGG GTTGGTGCCATAACAACCGACGATGATTATGGAAGACTAGCGCTCAACACATTTGCAATCCAGGCTGCTG AAAACAATGTGTGCATCGCCTTCAAAGAGGTTCTGCCAGCCTTCCTCTCAGATAATACCATTGAAGTGAG AATCAACCAGACTCTGGAGAAAATCATTGCCGAAGCCCAGGTCAATGTCATCGTGGTGTTTCTTAGGAAA TTCCACGTCTTCAATCTCTTCAACAAAGCCATCGAGAGGAAAATAAGTAAGATCTGGATTGCTAGTGATA ACTGGTCAACTGCTGCCAAGATTATCACCATTCCCAATGTTAAGAAGCTTGGCAAAGTAGTGGGCTTTAC TTTTAGGAGAGGAAATATGTCTTCTTTCCATTCTTTTCTTCAAACTCTGCATATGTACCCCAGTGACAAT AACAAACCCCTACATGAGTTTGCCATGCTTTTTTCTGCCTGTAAACACATCAAAGATGGTGATTTGAGCC AATGCATTTCCAACTATTCTCAGGCAACTTGGACCTATGACACTACCAAGACCATTGAGACCCATTTATT CAAGAGAAATGACTTCCTTTGGCATTATACTGAGCCAGGACTCATTCACAGCATTCAGCTTGCTGTGCTT GCCTTGGGCCATGCCATCCGGGATCTGTGCCAAGATCGAGACTGCCAGAAACCCAACGCCTTTCAGCCAT GGGAGCTACTTGCGGTGCTGAAAAACGTGACATTCACTGACGGAAAAAACTCATTTCATTTTGATGCCCA TGGGGATTTAAATACTGGTTACGAGGTGGTGCTCTGGAAAGAGACTAACGGCCTCATGACTGTCACGAAG ATGGCAGAATATGACCTGCAGCATGACGTCTTCATCACCACAAACCAAGAAACAAAGCATGAATTCAGGA AACTTAAGCAAATTCTATCTAAATGCTCCAAAGAATGCATTCCTGGTCAAATGAAGAAAGCCACAGGAAG CCAACACAGCTGTTGCTATGAATGTGTGAACTGCCCAGAAAACCACTACAGTAATGAGACAGACATGGAT CACTGCCTCGTATGCAACAACGAAACCCACTGGGCCCCAGTAAGGAGCACGATGTGCTTCGAGAAGGAAG TGGAGTATCTTGACTGGGATGACTCCTTGGCTCTCCTCCTCATTGCCCTCTCCCTACTTGGAATTGCCTT TGTTCTGGCCGTTGGCATAATATTTACAAGAAACCTGAAGACACCTGTGGTGAAATCATCTGGGGGATTA GTGGTCTGTTACGTGATGCTCGCCTGCCATGCCCTCAACTTTGCCAGCACAGGCTTTTTTATTGGAGAAC CACAGGACTTCACGTGCAAGACCAGGCAGACCCTGTTTGGCGTGAGCTTTACTCTGTGTGTCTCCTGTAT TTTGACGAAGTCCCTGAAAATTTTGCTAGCCTTCAGCTTCGACCCCACGCTGAAGACATTCCTGAAGTGT CTCTATAGGCCAGTCCCCATTGTCCTTACCTGCACGGGCATTCAAGTGGTCATTTGCACGCTTTGGCTGG TCCTGGCAGCACCTACTGTGGAAGAGAACACCTCCTTGCCTAGAGTCATTATCCTAGAATGTGAGGAGGG ATCTGCACTGGCGTTTGGCACCATGCTGGGCTACATTGCAGTTCTGGCCTTCATTTGTTTTGTATTTGCA TTCAAGGGGAGGAAGCTTCCTGAGAATTACAACGAAGCTAAGTTCCTGACCTTTGGGATGCTGATTTACT TCATAGCTTGGATCACATTCATCCCAGTCTATGCTACCACTTTCGGCAAGTATTTGCCCGCTGTGGAGAT CATAGTCATTCTGATATCCAACTATGGTATCCTCTGCTGTACATTCTTCCCCAAGTGCTACATTATTCTT TGCAAGCAAAAGACTAACACTAAGTCAGTCTTCCTCCAGATGGTTTACAATTACTCTGCTCACAGCGTGG ACAGCCTTGCCTTGAGTCACGTATCCCTGGACTCCGCCAGCCACAGTACTGCAACAACCAACCCGAGGCC TGGTAACAAGACTGCAGCCTGTCAGAATTACAAACATCTTCCTGTACAAGTGCTTGCTCACACAGGCATG GAAAAGACTATGCACGCGTCTAAAACTTTGCATCAGAAAAGAAGTTCAAGTATA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217339 representing NM_001271106 Red=Cloning site Green=Tags(s) MALSFVFITCFMILLDTSQSCHTPDDFVAITSPGHIMIGGLFAIHEKMLSSDDHPRQPQIQKCVGFEISV FLQTLAMIHSIEMINNSSLLSGVKLGYEIYDTCTEVTAAMAATLRFLSKFNCSRETVIFQCDYSSYVPRV KAIIGAGYSEISMAVSRMLNLQLMPQVSYESTAEILSDKIRFPSFLRTVPSDFYQTKAMAHLIRQSGWNW VGAITTDDDYGRLALNTFAIQAAENNVCIAFKEVLPAFLSDNTIEVRINQTLEKIIAEAQVNVIVVFLRK FHVFNLFNKAIERKISKIWIASDNWSTAAKIITIPNVKKLGKVVGFTFRRGNMSSFHSFLQTLHMYPSDN NKPLHEFAMLFSACKHIKDGDLSQCISNYSQATWTYDTTKTIETHLFKRNDFLWHYTEPGLIHSIQLAVL ALGHAIRDLCQDRDCQKPNAFQPWELLAVLKNVTFTDGKNSFHFDAHGDLNTGYEVVLWKETNGLMTVTK MAEYDLQHDVFITTNQETKHEFRKLKQILSKCSKECIPGQMKKATGSQHSCCYECVNCPENHYSNETDMD HCLVCNNETHWAPVRSTMCFEKEVEYLDWDDSLALLLIALSLLGIAFVLAVGIIFTRNLKTPVVKSSGGL VVCYVMLACHALNFASTGFFIGEPQDFTCKTRQTLFGVSFTLCVSCILTKSLKILLAFSFDPTLKTFLKC LYRPVPIVLTCTGIQVVICTLWLVLAAPTVEENTSLPRVIILECEEGSALAFGTMLGYIAVLAFICFVFA FKGRKLPENYNEAKFLTFGMLIYFIAWITFIPVYATTFGKYLPAVEIIVILISNYGILCCTFFPKCYIIL CKQKTNTKSVFLQMVYNYSAHSVDSLALSHVSLDSASHSTATTNPRPGNKTAACQNYKHLPVQVLAHTGM EKTMHASKTLHQKRSSSI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271106
ORF Size	2784 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271106.1, NP_001258035.1
RefSeq Size	3033 bp
RefSeq ORF	2787 bp
Locus ID	294394
UniProt ID	Q70VB1
MW	104.4 kDa
Gene Summary	Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids (By similarity).[UniProtKB/Swiss-Prot Function]

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