Pik3ca (NM_133399) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217450 representing NM_133399 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTTGATGCCCCCACGAATCCTAGTGGAAT GTTTACTCCCAAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCCACACTAGTCACCATCAAGCA TGAACTGTTCAAAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGATGAATCATCTTACATTTTC GTAAGTGTTACCCAAGAAGCAGAAAGGGAAGAATTTTTCGATGAAACAAGACGGCTTTGTGACCTTCGGC TTTTTCAACCCTTTTTAAAAGTAATTGAGCCAGTAGGCAACCGTGAAGAAAAGATCCTCAACCGAGAAAT TGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTCCAAGACTTCCGA AGGAACATTCTGAATGTTTGCAAAGAAGCCGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAA TGTATGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCTACAACAAGTTAGATAA AGGACAAATCATAGTGGTGATTTGGGTGATAGTCTCTCCAAACAACGACAAGCAGAAGTACACTCTGAAG ATCAACCATGACTGCGTGCCAGAGCAAGTCATTGCTGAGGCCATCAGGAAGAAGACCCGGAGCATGTTGC TGTCCTCGGAGCAGCTGAAACTCTGTGTCTTAGAATACCAGGGCAAGTACATTCTCAAAGTGTGTGGCTG TGATGAGTACTTCCTAGAGAAGTACCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGG AGGATGCCCAACTTGATGCTGATGGCCAAGGAGAGCCTGTACTCTCAGCTGCCGATCGATAGCTTCACAA TGCCATCCTACTCCAGGCGCATTTCCACAGCGACACCCTATATGAACGGGGAGACTGCTACGAAATCCCT CTGGGTTATAAATAGCGCGCTCAGAATAAAAATTCTGTGTGCAACCTATGTAAATGTAAATATTCGAGAC ATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAATACTC AAAGAGTCCCTTGTTCCAATCCTAGGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCC TCGTGCTGCCCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGCTAAGGAGGAGCACTGT CCGTTGGCCTGGGGAAACATAAACTTGTTTGATTATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGA ATCTCTGGCCTGTACCACATGGGTTGGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAATCCAAA TAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCAGTGTGGTGAAGTTTCCAGATATGTCT GTGATCGAAGAGCATGCCAATTGGTCTGTGTCCCGAGAAGCCGGATTCAGTTACTCTCATACAGGACTGA GTAACAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCCGAGCACTTTGTACCCG GGACCCACTGTCTGAAATCACTGAACAAGAGAAAGACTTCCTATGGAGCCACAGACACTACTGTGTAACT ATTCCTGAAATCCTACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGATGAAGTGGCCCAGATGT ACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCCATGGAGCTCCTGGACTGTAACTACCC AGACCCCATGGTTCGGAGCTTTGCTGTCCGGTGCTTGGAAAAATACTTAACAGATGACAAACTTTCTCAG TACCTCATCCAGCTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTGCTTGTGAGATTTTTAC TCAAGAAAGCACTGACAAATCAAAGGATTGGCCATTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAA TAAGACTGTCAGTCAGAGGTTCGGCCTGCTGTTGGAGTCCTACTGCCGTGCCTGTGGGATGTATCTGAAG CACCTGAACAGACAGGTAGAGGCCATGGAGAAGCTCATCAATCTAACTGACATCCTCAAGCAGGAGAAGA AGGATGAGACACAGAAGGTACAGATGAAGTTCTTGGTTGAACAGATGAGACAGCCAGATTTCATGGATGC TTTGCAGGGTTTTCTGTCCCCTCTAAATCCTGCTCATCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGA ATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCAGAGCTACTGT TTCAGAACAATGAGATCATCTTTAAAAATGGCGATGACTTACGGCAAGACATGTTAACCCTTCAGATCAT CCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTCGCATGCTACCTTATGGCTGTCTATCC ATTGGGGACTGTGTGGGTCTCATCGAGGTGGTGAGAAACTCTCACACCATCATGCAGATTCAGTGCAAAG GAGGCCTGAAAGGGGCACTGCAGTTCAACAGCCACACGCTGCATCAGTGGCTCAAGGACAAGAACAAGGG CGAGATATATGACGCAGCCATTGACCTGTTCACTCGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATC TTGGGAATTGGAGACCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATATAGATT TTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAACGGGAACGTGTGCCGTTTGTTTTGAC GCAGGATTTCTTAATAGTGATTAGTAAAGGAGCACAAGAGTACACAAAGACCAGAGAGTTTGAGAGGTTT CAGGAGATGTGTTACAAGGCGTACCTAGCAATTCGGCAGCATGCCAATCTCTTCATCAACCTTTTCTCCA TGATGCTTGGCTCCGGAATGCCAGAACTGCAGTCTTTCGATGATATTGCATATATTCGAAAGACTCTAGC CTTAGACAAAACTGAGCAAGAGGCTCTGGAGTATTTCACAAAGCAAATGAATGACGCACATCATGGTGGC TGGACAACAAAAATGGACTGGATCTTCCACACCATCAAGCAGCATGCATTGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133399
Insert Size	3207 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133399.2, NP_596890.2
RefSeq Size	6435 bp
RefSeq ORF	3207 bp
Locus ID	170911
UniProt ID	A0A0G2K344
Gene Summary	expression of the human homolog is associated with increased cell proliferation and decreased apoptosis and may play a role in ovarian cancer progression [RGD, Feb 2006]

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