Itga6 (NM_053725) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217453 representing NM_053725 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCGCCGGCCAGTTGTGCTTGCTCTACCTGTCCGCAGGGCTTCTGGCCCGGCTGAGCACAGCTT TCAACCTGGATACCCGCGAGGACAACGTGATCCGGAAATCGGGGGATCCCGGGAGCCTCTTCGGCTTCTC GCTCGCCATGCACTGGCAGTTGCAGCCGGAGGACAAGCGGCTGTTGCTTGTGGGGGCACCTCGGGCAGAA GCACTTCCCCTGCAGAAGGCGAACAGAACAGGGGGCCTGTACAGCTGTGACATCACCTCCCGGGGACCTT GCACACGAATTGTATTTGATAATGATGCTGATCCTATGTCAGAAAGCAAGGAAGACCAGTGGATGGGAGT CACTGTCCAGAGTCAAGGTCCAGGGGGCAAAGTGGTGACGTGTGCGCATCGATACGAGAAACGGCAGCAC GTTAACACGAAGCAGGAGTCCCGGGACATCTTCGGAAGGTGTTACGTCCTGAGTCAGAATCTCAGAATTG AAGATGATATGGATGGAGGAGACTGGAGTTTCTGTGATGGCCGATTGAGGGGCCATGAAAAATTTGGCTC TTGTCAGCAAGGAGTAGCGGCTACTTTCACTAAAGACTTTCATTACATTGTTTTTGGAGCCCCAGGGACT TACAACTGGAAAGGGATCGTTCGTGTAGAACAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAG ATGGGCCCTATGAAGTTGGTGGAGAGACTGATCATGACGAAAGTCTCGTGCCCGTTCCTGCTAACAGTTA TCTAGGCTTTTCTCTGGACTCAGGGAAGGGTATTGTTTCTAAAGATGACATCACTTTTGTATCTGGTGCT CCAAGAGCCAATCACAGTGGGGCTGTAGTTTTGCTAAAAAGAGACATGAAGTCAGCACATCTTCTCCTTG AGTATATATTTGATGGTGAAGGCCTGGCTTCTTCGTTTGGCTATGACGTGGCGGTGGTGGACCTCAATGC AGATGGGTGGCAAGACATCGTTATCGGAGCGCCACAGTACTTTGACAGGGATGGCGAAGTCGGGGGTGCG GTGTACGTCTACATTAACCAGCAAGGCAGATGGAGTAATGTGAAGCCAGTTCGTCTAAATGGGACCAAAG ACTCGATGTTTGGAATCGCAGTGAAAAATATCGGCGATATTAATCAAGATGGCTATCCGGATATTGCTGT TGGAGCTCCCTATGACGATCTGGGGAAGGTTTTTATCTATCATGGATCCCCGACTGGCATAATTACCAAG CCAACACAGGTTCTTGAGGGTACAACGCCTTTCTTCGGCTACTCAATCGCTGGGAACATGGACCTGGACA GGAATTCCTACCCTGACGTTGCTGTGGGTTCCCTCTCAGACTCAGTAACTATTTTCAGATCCCGGCCAGT GATTAACATTCTGAAAACCATCACAGTGACTCCTAGCAGAATTGACCTCCGCCAGAAATCCATGTGTGGG TCACCTAGCGGGATATGCCTCAAGGTTAAAGCCTGTTTTGAATATACTGCGAAGCCCTCCGGTTATAACC CTCCAATATCAATTTTGGGTATTCTTGAAGCTGAAAAAGAAAGAAGAAAGTCAGGGTTGTCGTCAAGAGT TCAGTTTCGAAACCAAGGTTCTGAGCCAAAGTATACTCAGGAGCTGACCCTGAACAGGCAGAAGCAGCGG GCATGCATGGAGGAGACGCTCTGGCTGCAGGAAAACATCAGAGACAAGCTGCGCCCCATCCCCATCACGG CTTCTGTGGAGATCCAGGAGCCCAGCTCTCGACGGCGGGTGAATTCACTTCCAGAAGTTCTTCCCATCTT GAATTCAAATGAAGCCAAAACAGTGCAGACAGATGTCCACTTCTTAAAGGAAGGATGTGGAGACGACAAT GTCTGTAACAGCAACCTTAAGCTAGAGTATAAATTTGGTACCCGAGAAGGAAATCAAGACAAATTCTCTT ACCTTCCAATTCAGAAAGGCATCCCAGAATTAGTCCTAAAAGATCAGAAAGATATAGCTCTGAAAATAAC GGTGACCAACGGCCCTTCTGATCCAAGGAATCCCCGGAAAGATGGTGACGATGCCCACGAAGCCAAACTC ATCGCAACGTTTCCAGACACCCTGACATATTCCGCCTACAGAGAGCTGAGGGCTTTCCCTGAGAAGCAGC TGAGCTGTGTTGCCAACCAGAATGGCTCCCAAGCTGACTGTGAGCTCGGAAATCCTTTCAAGAGAAATTC CAGTGTTACTTTTTATCTGATTTTAAGTACCACCGAGGTCACCTTTGACACCACAGATCTGGATATTAAC CTGAAGTTGGAAACAACAAGCAATCAGGATAATTTGGCTCCAATTACAGCGAAAGCAAAAGTGGTTATTG AATTGCTTTTATCGGTCTCTGGAGTCGCTAAACCTTCGCAGGTGTATTTTGGAGGTACAGTTGTTGGTGA GCAGGCCATGAAGTCTGAAGAAGAAGTAGGAAGTTTAATAGAGTATGAATTCAGGGTGATTAACTTGGGT AAACCTCTTAAAAACCTCGGCACGGCAACCCTGAATATCCAGTGGCCCAAGGAGATTAGCAATGGCAAAT GGTTGCTTTATTTGATGAAGGTCGAATCCAAAGGTTTGGAGCAGGTTGTCTGTGAGCCACACAATGAAAT AAACTTCCTCAAGCTGAAGGAGTCTCACAACTCAAGAAAGAAGCGGGAACTTCCTGAAAAACAGATAGAC GACAGCAGGAAGTTTTCTCTATTTTCCGAAAGAAAATACCAGACCCTCAACTGCAGCGTCAACGTGAGGT GTGTGAACATCAGGTGTCCACTGCGAGGGCTGGACAGCAAGGCCTCTCTCGTTCTACGCTCCAGGCTGTG GAACAGCACGTTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATCCTCGTGAGGGCGTCCATAGAT GTGACCGCCGCCGCTCAGAATATCAAGCTCCCTCATGCGGGCACTCAGGTTCGAGTGACGGTGTTTCCCT CAAAGACGGTAGCTCAGTATTCAGGGATAGCGTGGTGGATCATCCTCCTGGCTGTGCTTGCCGGGATTTT GATGCTGGCTCTATTAGTGTTTTTACTGTGGAAGTGTGGTTTCTTCAAGAGAAATAAGAAAGATCATTAC GATGCCACCTACCACAAGGCTGAGATCCACACTCAGCCGTCTGATAAAGAGAGGCTTACTTCCGATGCAT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053725
Insert Size	3222 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053725.1, NP_446177.1
RefSeq Size	5979 bp
RefSeq ORF	3222 bp
Locus ID	114517
Gene Summary	forms integrin with beta 4 subunit; involved in tumor progression [RGD, Feb 2006]

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