Srebf1 (NM_001276708) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217481 representing NM_001276708 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGCACATTTGAAGACATGCTTCAGCTCATCAACAACCAAGACAGTGACTTCCCTGGCCTATTTG ATGCCCCCTATGCTGGGGGTGAGACAGGAGACACAGGCCCCAGCAGCCCTGGTGCCAGCTCTCCTGAGAG CTTCTCTTCTCCTGCTTCTCTGGGCTCCTCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTGCA CCCTTGTCCCCTCCACCATCGGCACCCACTGCTGTAAAGATGTACCCGTCCGTGCCCCCCTTCTCCCCTG GGCCTGGAATCAAAGAGGAGCCAGTGCCACTCACCATCCTGCAGCCCCCAGCACCACAGCCATCGCCAGG GACCCTGTTGCCTCCGAGCTTCCCTCCTCCACCTGTGCAGCTCAGCCCTGCTCCTGTGCTGGGGTACTCA AGCCTGCCTTCCGGCTTCTCAGGAACCCTTCCTGGGAACACCCAGCAGACGCCATCTAGCCTGCCACTGG GCTCCACGCCAGGAATCTCGCCCACCCCCTTACACACCCAGGTCCAGAGCTCGGCCGCCCAGCAGCCGCC GCCAGCCTCAGCAGCCCCTAGAATGAGCACTGTGGCCTCACAGATCCAGCAGGTCCCCGTTGTACTGCAG CCACACTTCATCAAGGCAGACTCGCTGCTGCTGACAGCTGTAAAGACAGACACAGGAGCCACAATGAAGA CCGCAGGCATCAACACCCTGGCTCCTGGCACAGCCGTGCAGGCAGGCCCCTTGCAGACCCTGGTGAGTGG AGGGACCATCCTGGCCACAGTACCACTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTAGCAGCT GGTGGCAAGGCCCTGGGCTCAGCTCAGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAAGC GCTACCGTTCCTCTATCAATGACAAGATTGTGGAGCTCAAGGACCTGGTGGTGGGCACTGAGGCAAAGCT GAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATCCGCTTCTTACAGCACAGCAACCAGAAACTC AAGCAGGAGAACCTGACCCTGCGAAGTGCTCACAAAAGCAAATCACTGAAAGACCTGGTGTCAGCTTGTG GCAGTGGAGGAGGCACAGATGTGTCTATGGAGGGCATGAAACCTGAAGTGGTAGAAACGCTGACCCCTCC ACCCTCAGACGCCGGCTCACCCTCCCAGAGTAGCCCCTTGTCCTTGGGCAGCAGAGGCAGCAGCAGTGGT GGCAGTGACTCTGAGCCCGACAGCCCAGCCTTTGAGGATAACCAGGTGAAAGCCCAGCGGCTGCCTTCAC ATAGCCGAGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGTGTACTGGTCTTCCTGTGTCTGACCTGCAA CCCATTGGCCTCACTGTTTGGCTGGGGCATCCTCACTCCCTCTGATGCTTCGGGTGTGCACCGTAGTTCT GGGCGCAGCATGCTGGAGGCCGAGAGCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCTAG TCTGGCTGGCCAATGGACTACTAGTGTTGGCCTGCTTGGCTCTTCTCTTTGTCTACGGGGAACCTGTGAC CAGGCCACACTCCGGCCCGGCTGTACACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCCGG GGAGATTTTGCCCAGGCCGCTCAACAGCTGTGGCTGGCCTTGCAAGCCCTGGGCCGGCCCCTGCCCACCT CAAACCTGGATCTGGCCTGCAGCCTGCTTTGGAACCTCGTCCGCCACCTGCTGCAGCGTCTTTGGGTGGG CCGCTGGCTGGCAGGCCAGGCTGGGGGCCTGCAGAGGGACTACAGGCTGAGAAAGGATGCTCGTGCCAGT GCCCGAGATGCGGCTGTCGTCTACCATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGGCC ATCTTGTTGCTTCTAACCTGGCACTGAGTGCCCTTAACCTGGCTGAGTGTGCAGGAGATGCTATATCCAT GGCAACACTGGCAGAGATCTACGTGGCAGCTGCCCTAAGGGTCAAAACCAGCCTCCCCAGAGCCTTGCAC TTCTTGACACGTTTCTTCCTGAGTAGTGCCCGCCAGGCCTGCCTGGCACAGAGTGGTGCAGTGCCTCTTG CCATGCAGTGGCTCTGCCACCCTGTAGGTCACCGTTTCTTCGTGGATGGGGACTGGGCTGTACACGGTGC CCCCCAGGAGAGTCTGTACAGCGTGGCTGGGAACCCAGTGGATCCACTGGCCCAGGTGACCCGACTATTC TGTGAACATCTCCTGGAGCGAGCATTGAACTGTATCGCTCAGCCCAGCCCAGGGGCAGCTGATGGAGACA GGGAGTTCTCAGATGCTCTTGGATATCTACAGTTGCTAAATAGCTGTTCTGACGCTGTCGGAGCTCCTGC GTGCAGCTTCTCTGTCAGTTCCAGCATGGCTACCACCACTGGCACAGACCCAGTGGCCAAGTGGTGGGCC TCACTGACAGCCGTGGTGATCCACTGGCTGAGGCGGGATGAGGAGGCAGCTGAACGCTTATACCCACTGG TAGAGCACATTCCCCAAGTGCTGCAGGAAACTGAGAGACCCCTTCCCAGGGCAGCTCTGTACTCCTTCAA GGCTGCCCGGGCTCTGCTGGACCACAGAAAGGTGGAATCCAGCCCAGCCAGCCTGGCCATCTGTGAGAAG GCCAGTGGGTACCTGCGGGACAGCTTAGCCTCTACATCAACTGCCAGTTCCATTGACAAGGCCATGCAGC TGCTCCTGTGTGATCTACTTCTTGTGGCCCGCACCAGCCTATGGCGGCGCCAACAGTCAGCAGCTTCAGC CCAGGGAGCTCACGGTACCAGCAATGGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATGAC CTGAGCAGCCTGAGGCGCTTGGCACAGAGCTTCCGGCCTGCTATGAGGAGGGTCTTCCTACATGAGGCCA CAGCTCGGCTGATGGCAGGAGCAAGTCCTGCCCGGACACACCAGCTCCTGGACCGCAGTCTGCGGAGGCG GGCAGGTTCCAGTGGCAAAGGAGGCGCTGCAGCTGAGCTGGAGCCTCGACCCACATGGCGGGAGCACACA GAGGCCTTGCTGTTGGCCTCCTGCTATCTGCCCCCTGCCTTCCTGTCGGCCCCCGGGCAGCGAGTGAGCA TGCTGGCTGAGGCAGCGCGCACCGTGGAGAAGCTTGGCGATCACCGGCTCCTGCTTGACTGCCAGCAGAT GCTCCTGCGCCTGGGTGGCGGGACCACTGTCACTTCCAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276708
Insert Size	3333 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276708.1, NP_001263637.1
RefSeq Size	4264 bp
RefSeq ORF	3333 bp
Locus ID	78968
UniProt ID	P56720
Gene Summary	transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate 5' exon structure, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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