Hephl1 (NM_001191993) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564835
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217498 representing NM_001191993 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGAAGCAGCCAGGTGGCTGCATCCTTCTTGAGTTCCTGGGTCTGCTTGGACTGGTTGGTGCAG TCACCAGAACATACTACATTGGGATTGTGGAAGAATACTGGAACTATGTGCCTCAAGGGAAGGATGTTAT TACTGGGAAGAGCTTCTCGGAAGACAAACTTGCGACCTTATTTCTGGAGCGAGGGCCCAACAGGATAGGT GGCATCTACAAGAAGGCGGTTTATAGACACTTCACAGATGGGAGCTACTCCACCGAGATCCCTAAGCCCC CCTGGCTGGGGTTCCTGGGTCCCATCCTGAGGGCAGAAGTGGGCGACGTGATCGTTATCCACTTGAGAGC CTTATATCCAGATGGGACGTCTGGAAAGAACAAGGAGGATGACATGGTCCCCCCTGGCAAAAACTACACC TATGTCTGGCCGGTGAGAGAAGAATATGCACCCGCTCCGGCAGATGCCAACTGCCTGACCTGGGTGTACC ACTCACACATTGATGCCCCCAAGGATATCTGCTCTGGGCTAATTGGACCCTTGCTGGTGTGTAAGGAAGG TGTACTGAACAGACACTCAGGGGCGAGGACTGACGTGGACCGAGAGTTTGTCATCATGTTCACTCTGGTG GATGAGAACCAGAGCTGGTACCTGGATGACAACATCAGGCAGTTCTGCACTAATCCCAGTTCTGTTGACA AAAGCGACGCTGTTTTCCAGAGGAGCAACAAGATGCATGCCCTCAATGGATTCCTTTTTGGAAACTTGCC CGAGCCTGAGATGTGTGTTGGTGAATCTGTGTCCTGGCACCTGTTTGGGATGGGGAATGAGATAGACATC CATTCCATCTATTTTTATGGTAACACCTTCATCACCAGAGGGCACCGGGCGGATGTTGTCAACCTGTTTC CAGCCACGTTCCTCACAACAGAAATGATAGTGGAGAATCCTGGGAAATGGATGATAACTTGCCAAGTTAG TGACCACCTGCAAGCTGGCATGCTGGGACAGTACAATGTGGGTAACTGCAGGGGTAATGCTCCTCACCCG AAGATGCGGGGCCAACAGAGGCGCTACTTCATAGCGGCTGAAAAAGTCCTCTGGGATTACAGTCCCCAGG GCTACGATAAGTTCACTGGATTTCCCCTGAATACATCTGGCAGTGACTCTGCAGTGTACTTCACACAAGC TGACAACAGAATAGGAGGAAAATATTGGAAGGCTCGGTACACGGAGTACGTGGATGCCACTTTCAGTAGA AGAAAGATGCCCTCAGATTCAGAAGCCCATCTTGGAATCCTCGGTCCAGTCATCAAGGCAGAGGTGGGTG ACATCCTGCTAGTGACCTTTGCCAACAAAGCTGACAAGGTCTACAGCATTTTACCCCATGGTGTGTTCTA TGACAAGGCATCGGATGCAGGGCCGAATGTTGATGGATTTCTGAAACCAGGGGCTCATGTCAAGCCCGGT GAAACCTTCACCTACCGGTGGACAGTGCCAGAAAGCGTCAGCCCGACAGATGACGACCCCCCGTGCCTGA CCTATCTTTATTTCTCAGCGGTACAGCCGATCAAGGACACTAGTGCTGGGCTTGTGGGGCCTTTGTTGGT CTGCAAAAAGGGCACGCTCAATGCCGATGGGACACAGAAAGGAATAGACAAGGAGTTTTACCTACTGTTC ACAGTCTTTGATGAGAATTTCAGCAGCTATCTTGATGAAAACATCCAGAAGTTTACTTGGCATCCCTTCA GTGTCGACAAAGAAGACAAGGAGTTCGTGAAATCCAACCGGATGCATGCTATTAATGGCTACATGTATGG CAACCAGCCAGGCCTAAGCATGTGCAAGAAGGACAGAGTTTCCTGGCACTTAATTGGAATGGGCACCGAC ACAGACATGCATGGAGTTTATTTCCAAGGCAACACCATCCACCTACGAGGGACTCATCGGGACTCCTTGG CTCTGTTTCCCCATATGTCCACAACGGCATTCATGCAGCCAGACCATTCAGGTATTTTCAAGGTGTTCTG CTCCACCTTGCACCACTTCACGAGAGGCATGGGTCAGATCTATGAGGTCAGCAGCTGTGGCAACAGGGAC CCTTCTGAGCCGCCCTACGGGATGCTAAGAACCTTTTACATCGCCGCTGAAGAGGTAGAATGGGATTATG CCCCTAACAAAAACTGGGAGTTCGAAAAGCAGCACTTGGACGCAGGAGGGGAAAGACATGGAGATATATT TATGAACCACACTGAAAACTGGATTGGCTCTCAGTACAGGAAGGTGGTTTATAGGGAATACACAAACGGA GAATTTGTGGAGATTAAAGCCCGGCCACCTCAAGAGGAGCACCTTCAACTCCTGGGACCAATGATCCATG CTGAGGTGGGGGACTCCATTCTCATTATCTTTAAGAACAAAGCCAGCCGGCCCTACTCCATCACAGCCCA GGGTGTGGAGGACTCAGACAGTGGAAAATTCCTTGACGTGCCAGTCACAAAGCCAGGGGAAATAAAAACG TACAGATGGAATGTCCCAAAGAGATCAGGTCCTGGGCCCTCTGATCCCAACTGTATCCCGTGGGTGTACT TTTCAACAGCAAACTTTGTGAAGGATACGTACAGTGGGTTGATGGGGCCTCTGATCACATGCCGAGAGGG GGTGCTAAACGAGAAGGGGAGAAGAAGTGACGTTGACTACGAGTTTGCGCTGCTCTTCCTGGTCTTCAAT GAGAACGAGTCGTGGTATCTGGATGACAACATCAAGAAGTACCTCAACAAAGATCCACGGGATTTCAGGC ACACCGATGACTTTGAAGAGAGCAACAGAATGCACGCCATTAATGGAAAGATTTTTGGAAACCTCCCTGG CCTCATAATGACTGAAGACTCAATGACCAACTGGTATTTGCTGGGCATCGGAAGCGAAGTGGACATACAC ACGATCCATTACCATGCAGAGAGCTTTCTGTTCAAAATTGATAAGTCCTACCGAGAAGATGTGTATGATC TCTTTCCTGGGACATTTCAAACCATCGAGCTGTTTGCTGATCACCCGGGGACGTGGCTCCTTCACTGCCA CGTGTCCGACCACATCCATGCTGGCATGGAGACCACGTACACTGTCCTCAGGAACATAGACAACAGGATT CCATATTCTACCAAGTCCCCTTCTGGAGCAGGACCCCATGCAGTCACAGTGCCCTCCCAGGAACAACCTG GACAGGAAGACCTCTACTTCTTTGGCAAGAGTCTGCGTCCAAGAGGAGCCAAGGCAGCGCTGGTCATCCT TTTCATCCTGGGACTCCTCCTCCTGGTGGCCACCGTGATTCTCGCCCTCAGACTCCGCTCTTCAAGGAGG CAGATGGCTTACAGGGAGGTCCAGTCCTGTGCACTCCCCACAGATGCTCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191993
Insert Size	3414 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191993.1, NP_001178922.1
RefSeq Size	3414 bp
RefSeq ORF	3414 bp
Locus ID	500946

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