Ddb1 (NM_171995) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217500 representing NM_171995 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACCGCAGTGAACGGCTGTGTGACCGGACACTTTA CTTCAGCGGAAGACTTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACCGCAGA AGGCCTACGGCCAGTCAAAGAGGTGGGGATGTATGGGAAGATCGCAGTTATGGAGCTCTTCAGACCCAAG GGGGAGAGTAAAGACCTGCTGTTCATCTTGACTGCTAAGTACAATGCCTGCATCCTGGAGTATAAGCAGA GTGGTGAAAGCATTGATATCATTACAAGGGCCCATGGCAATGTCCAGGACCGTATTGGTCGCCCCTCAGA GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTCCGACTCTACGATGGCCTTTTC AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAAGAATTGCATG TCATCGATGTCAAGTTCCTGTATGGTTGTCAAGCGCCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG GCGGCACGTGAAAACCTATGAGGTGTCTCTTCGGGAGAAGGAGTTCAACAAGGGCCCTTGGAAGCAGGAG AATGTAGAAGCCGAAGCTTCAATGGTGATCGCAGTCCCGGAGCCTTTTGGAGGTGCCATCATCATTGGAC AGGAGTCAATTACCTATCACAATGGTGATAAATACCTGGCAATCGCTCCTCCTATCATTAAGCAAAGCAC TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCACGGTACCTTCTGGGAGATATGGAAGGACGGCTT TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACCGTCACCCTCAAGGACCTTCGAGTGGAAC TCCTTGGAGAGACCTCCATTGCAGAGTGCCTAACATATCTTGATAACGGTGTTGTGTTTGTCGGGTCTCG CCTGGGTGATTCCCAGCTCGTAAAGCTCAATGTCGACAGCAATGAACAAGGCTCCTATGTAGTGGCTATG GAAACCTTTACCAACTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGAATTGGAATCCA TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCACTGCGGTCTGACCCAAGTCGGGAG ACTGATGACACTTTGGTACTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAATGGAGAGGAAGTGG AAGAAACAGAATTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGCGGCAATGTGGCTCATCAGCA GCTTATCCAGATCACCTCAGCGTCTGTAAGGTTGGTCTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG AAGGAGCCCCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTAGTTGCTGTGGGAA GGGCACTATACTATCTTCAGATCCATCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA AGTGGCTTGCTTGGATATCACCCCACTAGGAGAGAGCAATGGCCTGTCCCCACTTTGTGCCATTGGTCTC TGGACAGATATTTCAGCTCGTATCTTGAAGCTCCCATCCTTTGAACTACTGCACAAGGAGATGCTGGGTG GAGAGATCATCCCTCGATCTATCCTGATGACCACTTTTGAGAGCAGCCACTACCTCCTTTGTGCCTTGGG AGATGGGGCTCTCTTCTACTTTGGGCTCAACATAGAGACAGGCTTACTGAGTGACCGTAAAAAGGTGACT TTGGGTACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCAG ACCGGCCCACTGTCATCTATAGCAGCAATCATAAGTTGGTCTTCTCCAATGTTAACCTCAAGGAGGTGAA CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC ATTGGCACCATCGATGAGATCCAGAAACTCCATATTCGCACGGTTCCTCTCTATGAATCTCCCAGGAAGA TCTGTTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTTCAAGATACCAGTGG AGGCACTACTGCTTTGAGGCCCAGTGCTAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAGCTC TTCTCTAGTAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAGGTGGAAGTGCACAACCTCCTCATCA TTGACCAGCATACTTTTGAAGTGCTTCATGCCCATCAGTTTCTACAAAATGAGTATGCCCTCAGCCTGGT CTCCTGCAAATTGGGTAAAGACCCCAACACATACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG GCGGAGCCCAAACAGGGTCGTATTGTGGTCTTCCAGTATTCGGATGGAAAACTACAGACTGTGGCCGAAA AGGAAGTAAAGGGGGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCTAGCATCAACAGCAC GGTGCGGCTCTATGAATGGACCACAGAAAAGGAACTTCGCACTGAGTGCAATCACTACAACAACATCATG GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGATCAGTGCTACTGCTTG CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAGAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGACTTCTACCCCTACCCAGGGCTC GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCGGAGAGCTGGTACAACCTC CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGTGTGGGCAAGATTGAGCACTCCTTCTGGA GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGACCTGATTGAAAGTTT CCTAGATATCAGCCGCCCCAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_171995
Insert Size	3423 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_171995.2, NP_741992.2
RefSeq Size	4260 bp
RefSeq ORF	3423 bp
Locus ID	64470
Gene Summary	may be a DNA damage repair protein [RGD, Feb 2006]

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