Med23 (NM_001277054) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Crsp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217602 representing NM_001277054 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCAATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG CTTTCCCAGGCATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTTATTAGCTGTTTGGCAGCCTT CCGGCAGTTTTGGAATGGACTTTCTCAGGAGTCTCATGAACAGTGTGTCCAGTGGATTGTTAAATTTATT CACGGCCAACATAGCCCGAAAAGGATTTCCTTTCTCTATGACTGCTTAGCCATGGCAGTTGAGACTGGTC TTCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACACTCTTGAATGGGAAAGAACACAGCT TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTATAAGGGTGTTCGAGACCTC CTGAAAGTGATTTTGGAGAAAATCTTGACAATCCCGAATACTGTAAGCTCTGCTGTTGTCCAGCAGCTTC TGGCAGCAAGAGAGGTTATAGCGTATATCTTGGAACGAAATGCCTGCCTGTTGCCAGCCTACTTTGCAGT CACAGAGATCAGAAAATTATATCCCGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC TTTGTGGATACCTTCAGGCCCACAGCGAGGATAAACTCCATTTGTGGCCGTTGCAGTCTTCTGCCAGTTG TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTCCGCTTTCCCTTGAAAGG CCTTTTGCCATACGATAAGGATCTGTTTGAACCCCAGACTGCTTTGTTGAGATATGTACTGGAGCAGCCT TACTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAACAAGCAGCACAAGCAGCGCTGCCCTGTGCTGG AGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCTGAGACCGAGGAGAAGTTTGACGATGG GGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCC AGTTTCCCACATATGGTTCTGTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACC ATCTGATGTGGGTTCTGCTGCAGTTCATCTCGGGAAGTATTCAGAAAAATGCGCTTGCTGACTTCCTCCC TGTCATGAAGCTCTTTGACCTGCTGTACCCAGAGAAGGAATGTATCCCAGTTCCTGATATTAACAAACCA CAGTCAACACATGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGACAACT CCAAGCTGCAGATCCCCATACCCCACTCCCTGAAGCTGCACCACGAGTTCCTACAGCAGAGTCTGAGAAA CAAAAGTTTGCAGATGAATGACTATAAGATCGCCCTGCTGTGTAATGCGTATTCTACAAATTCAGAGTGT TTCACCTTACCTATGGGCGCTCTGGTAGAGACCATTTATGGCAATGGAATTATGAGAGTGCCTCTCCCCG GGACAAGCTGTCTGGCCTCAGCATCAGTAACTCCCTTACCAATGAACCTCCTGGATTCTCTGACAGTGCA TGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACAAAATCCAGTGTC GCATTGGCTCCAGCCCTGGTGGAAACTTACAGTCGTTTACTGGTCTACATGGAAATAGAATCTTTGGGCA TCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCATGCCTGGGGCATCCTGCACACGCT GCTGGAGATGTTCAGCCACCGGATGCACCACATTCAGCCACATTACCGAGTTCAGCTCCTGAGCCATCTC CACACCCTGGCTGCAGTTGCACAAACCAACCAGAACCAGCTCCATCTGTGCGTAGAAAGTACTGCGCTGA GGCTCATCACGGCGCTGGGGAGCTCCGAGGTCCAGCCGCAGTTTACACGCTTCCTCAACGATCCCAAAAC AGTGCTGTCCGCAGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTGGCCAGAGCAACCCACGTC ACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGA CTTTTACTCCTCATAACTGGGCTTCGCACACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCATTCTTCAA GCAAAATAATGTACCTCAGGAAAGCCGTTTTAATCTGAAAAAAAACGTGGAAGAAGAGTACAGGAAGTGG AAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCCATGCAAGGCTTCCCTCCGCTCTTCCTCT GTCTCCTCTGGAAAATGCTCCTGGAAACGGATCACATTAGTCAGATTGGGTACAAAGTACTAGAGAGAAT TGGGGCCAGGGCCTTGGTGGCCCACGTGAGAACCTTTGCAGATTTCTTGGTGTATGAGTTCTCTACATCA GCAGGAGGTCAGCAACTCAATAAATGCATTGAGATTCTCAATGACATGGTGTGGAAGTACAACATTGTCA CACTGGACAGACTAATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCTCAGGTTTGTTATTT CATAATTCAGTTGCTATTGCTAAAACCAAATGACTTCCGAAACCGAGTGAGTGACTTTGTGAAGGAGAAC TCCCCAGAGCACTGGCTGCAGAGCGACTGGCACACCAAACACATGAGCTACCACAAGAAATATCCAGAGA AGTTGTACTTTGAAGGCCTGGCAGAACAGGTTGACCCTCCTGTACCAATTCAGTCCCCGTATCTGCCCAT CTACTTTGGAAACGTGTGTCTCCGGTTTCTTCCAGTATTTGATATAGTGATCCACAGGTTTTTAGAGTTG CTGCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTGTACAAATTCCACGACCGGC CGGTGACTTACCTGTATAACACTTTACACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACG AAAACTCGTCCATGCCATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGACACC TACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCAGAGGACTCCTACTACTGCAAGC TGATTGGCCGGCTGGTAGACACCATGGCTGGCAAGTCTCCGGGACCCTTCCCAAACTGTGACTGGAGATT CAATGAGTTCCCCAACCCTGCTGCCCATGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCAGTT CCAGGCAAAGATGTCGGGAATGCCCTTCTCAATGTTGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGA ACATCACAGCATGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGAACCATACTGGATTGTGCT TCATGACCGAATCGTGAGTGTAATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTTGGTTATCCG TTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCTGAGATGAGCTGCAGCTACACGTTAGCGC TGGCTCACGCCGTGTGGCACCACTCCAGCATCGGACAGCTCTCGCTTATCCCGAAGTTTCTCACTGAAGT GCTTCTTCCCGTGGTGAAGACTGAATTCCAGTTGCTTTACGTGTACCATCTCGTAGGGCCATTTTTACAG CGATTCCAGCAGGAGAGAACCCGGTGCATGATCGAGATTGGAGTGGCATTTTATGACATGCTGCTGAATG TAGACCAGTGCAGCACCCACCTCAATTACATGGACCCCATCTGCGACTTCCTATACCATATGAAGTATAT GTTCACTGGAGACAGTGTGAAGGAGCAGGTAGAGAAGATCATCTGTAATTTAAAGCCAGCTTTGAAGCTT CGTCTTCGGTTCATCACGCACATCAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAGCAGCGGGT CTCCAGCCCCTCAGGCCAACCAGGTGCCCACAGCTCTGCCCGTGACTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277054
Insert Size	4113 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277054.1, NP_001263983.1
RefSeq Size	4727 bp
RefSeq ORF	4113 bp
Locus ID	309565

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