Shroom4 (NM_001191730) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1563434
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217642 representing NM_001191730 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACCGGCCGGGGTCCTTCCAGTACGTCCCGGTGCAGCTGCAAGGGGGGGCGCCCTGGGGCTTCA CCCTGAAGGGGGGCTTGGAGCACTGCGAGCCGCTCACGGTGTCGAAGATTGAAGATGGAGGCAAGGCAGC CTTGTCCCAGAAGATGCGGACTGGTGATGAACTGGTAAATATCAATGGCACTCCATTGTATGGCTCCCGC CAAGAGGCCCTCATCCTCATCAAAGGCTCCTTCAGGATCCTCAAGCTGATTGTCAGGAGGAGAAACACCC CTGTGAGTAGGCCGCACTCATGGCATGTGGCCAAGCTATTGGAGGGGTGCCCTGACGTGGCCACCACCAT GCATTTCCCTTCTGAAGCCTTCAGCTTGTCCTGGCATTCTGGTTGCAACACAAGTGACGTGAGTGTACAG TGGTGCCCACTCTCCCGGCATTGTAGCACTGAGAAAAGCAGCTCTATTGGCAGCATGGAGAGCCTGGAAC AGCCAGGCCAAGCTACCTACGAGGGCCACCTCTTACCCATTGAGCAGAACATGTACCCCAGCCAGCGTGA CTCAGCCTACAGCTCCTTCTCAGCCAGCTCAAATGCCTCTGACTGTGCCCTTTCCCTTAAGCCAGAGGAA CCACCCTCTACAGACTGTGTCATGTCAGGCCCAGGGCCGACTAAAGTCACTGATGACCAAGCTAATGTAT CTGAGACCTCAGGAAGTAGCCATAGTACCAGTGGGGGCCACATGACCTCCACTTCCCATGCATCATCCTG CTCAGAAGAAGGGCACCACTCAGGACCTGCCAAGATGGTGCGGGGTCCACCAGAGCCTCCAGTGAGGCGG GACAGCCTTCAGGCCTCTAGAGCCCAACTTCTCAATGGAGAGCAGCATCGTGCATCTGAACCTGTGGACT CCTTACAGCAAAAGGAAAAACCAGCCTTAGAGACAATGCTGCCCCCAAGGAGCTCTAACCAGTTCTGCTG CATTAGTGGGCAAGACCAGGTGACAGGTGAGGACCACCAGAACTGTGAGCTCAGCAAGCCTTCTGAATCC AGCCAGGATGACTGTGACCATCTACTGATAGAGGGCTCCTCCAAAGCTCTTGATTCTCCAAAGGCCCATG ACAAAGGTTCCAACAAAGAGTTTGGCCTGCTTAAGGAGGCTTCAGCAGATCTGGCTAACACTTTGTCTTT TGGTGCCATCCCGCACCTCCGAGGAAGCATAGAACATCGCCATAGTGCTCCTGAACAGCTGTTGGCATCC CACTTGCAGCAGATGCACCTTGATTCCAGAGGCAACAAGGGGATGGAGTTGCCAACTGGGCAGGATGGAC ACCAGTGGACCATGTCCCCTTTGCACAACAACCCTAAAGGGAAGAAAAGTCCAGGTCTCCCCACAGGAGG TACCCAGGACCAGACCAGCAAAGAAAGGAAGATCAAGCCCACAGATGATAAGCTGATGGTTTCTGCAAAC CAGAGCCAAAGTGATGCCCTACTTGGAGAGGTCGATGGACACCCATCAGAACGAACTTACCTAGACTCAA ACCGAGCAAGCAGAGCAAGCAGTGACTTGACTGGCCAGCAGCCCTCTGCCACTTGCTCCCCTGTTCAACA AACCAGAGACTTCTTTTCAGCCAATAAAGTAGTTGGTCACACTGAAGCAACTGAAGAAGGTGACAATGAG CCTAAGGAAGGTGGCCGATTGGGTGGCAGAAGAAGTGGAGGCCCCCGAGGTCGCTCCATCCAAAACCGAC GGAAGAGTGAGAGGTTCGCTACCAATCTACGGAATGAAATTCAGAGGAGGAAAGCCCAGCTCCAGAAAAG TAAGGGCCCCTTGTCACAATTGTGTGACACTAAGGAAGATGTGGAAGAGACCCAAGAGCCTCCAGAAAGC CCTCCACTCCCTGCTTCTAACTCATCCCTTCTGCCTTCATATAAAAACGTCCCTAGCCATGGAGATCAGC TCTTTAACAAAAGCATGATTCTCAGGGCTAGGTCTTCAGAATGCCTCAGCCAAGCCTCAGAGAGCTCTAA AGCTAGGGTAGGCTTAGAGGGAAGAATGAGCCCTGGCCAGAGGTCTGGCCAGTCTTCTTTGGCCCTGAAC ACATGGTGGAAGGCATCTGACTCATCCACCTTAGACACTGAGAAAGCAAATGCTCACCACGGGGTCTGTA GAGGTCACTGGAGATGGTCTCCAGAACATAACGCACAGCCACAGGTGGCACTCTCCACAGAGGGTCCTTC CAACCCAGATGACAACAAAGAATTGAAGATACCTACTCCTCAGGCTGGGGATGAAGCCATCCTCATGCCA TTTGCAGACAGAAGAAAGTTCTTTGAAGAGAGCAGCAAATCTTTATCTACATCTCATTTGCCAGGCTTAA CCACTCACAACAAGCCATTTATCCAGAGACCAAAACCTATAGATCAAAACTTCCAGTCAGTGAGCTATAG GGAATTACGATGCCATCCTATGGACCAATCATATCATTCTGCAGACCAACCATACCATGCTGCTGACCAA TCATATCATTCCTTGTCACCACTTCAGTCAGAAGCTCCCACTTACCCAGACTGCTTTGCTCCCAAAGGCG TAGATAATTCCCTGTACTGTAAGCCAGTGCACCATGGGGATTGTGATTACCACAGGACCTGTTCTCATCC CTGCAGTGCTCAGGGAACTGTAATGCATGATCCTTGCATTTGCTGTTCTGGAGAAATTTGCCCTGCCTTG CTAAAGAGAAATTTGTTGCCAAAATGCCACAACTGTAGGTGTCACCATCACCAATGCATCCGGTGTACAG GTTGCTGTCACAGTCCTCAGCATGGTGCCCATGAGGACAGCAGTATGGCATCTGGCAATGCTTGGAAATC CAGGAAGGCAGCCATACAGGAATTTCCTGTGGACAAGTGGAAGCCAATAACAGGAAACAGGAAAACTAGT CATTCCGGGAGGGAAATGGCTCATTCTAAGGCCAACTTGTCATTGTCTGCTCCCTTCCGGCCTCACATTG AGAACTCAGCACTGGACTTGTCAAACTACCGAGCAGTCTCTTCGCTTGACCTCCTTGGAGACTTCAAACA TGCCTCGAACACTCCAGAGGAATCTTCAGTTTATGAGGATGAGAGCTCCGTTGCCTCCATGCCCCGCCCA CTGCGGAGCCGAGCCTTCTCTGAGAGTCACATCAGTTTAGAGCCACAAAACACCCGGGCCTGGGGGAAAC AGCAAAGGGAATCATTTAGTAAAGGCAGTGAGACTCAGCCAGATACTCTTGGGGCCCGAAAGAAGGCCTT TCCTCCCCCTCGCCCTCCTCCTCCCAATTGGGAAAAATATAGGCTCTTTCGTGCAGCGCAGCAGCAGCAG CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAATGTGAAAAGGAAGAAGAGGAGG AGAAGGAGCAAGAGGAGAAGGGAGAGAAAGAAGAGGACCTACCGCCCCAGTATTTTAGTTCAGAAACCAC TGGATCATGTGCCCCTATTACAGAGGAGCAGCCACAGTCCCTGAAGATGGGCCACCAGGAAGCCTCAAGG CAGAGTTCACAGAGCCTCCAAGAGCAAGAGGCCTATGCTGTGCATCCCAGCAATTTTGTGCCTGCAGTAA GGAGCTACCCTGTAACCCAGCCCGAGAAAGCTCAACCTCCTTGCTATTACGGCGCTCATGGACTGTGGAG GACAACTGAGCAAGAAGCTACTGTGACCCCAAAACAAGAGTTTCAGCACTTCTCACCTCCTACAGGGGCC TCAGGAATCCCTACCTCTTACTCAGCTTATTATAATATCTCTGTGGCCAAGGCAGAACTGCTGAACAAGT TAAAACAGCAGCCTGAGATGGCTGAGGCAGGCCTGGGAGAGGAGGGAGTTGACTATGAACTGGCTCAAAA AAAGATTCAGCTCATTGAAAGCATTAGTAGAAAACTTTCTGTCCTGCGGGAGGCCCAGCGTGGGCTACTG GATGACATCAATGCCAACGCTGCCCTTGGAGAGGAGGTGGAGGTCAACTTAAAAGCTGTCTGCAAGTCCA ATGAGTTGGAAAAGTATCATTTGTTTATTGGAGACCTGGACAAAGTGGTCAACCTGTTGTTGTCACTCTC TGGACGACTGGCCCGGGTAGAGAATGCTCTCAACAGCATCGATGCTGAGTCCAACCAGGAGAAGTTGGTG CTGAGAGAGAAGAAGCAGCAGCTGACGAACCAGTTGGCAGATGCCAAAGAGCTGAAGGAGCATGTGGACC GCAGGGAGAAGCTGGTGTTCAGCATGGTCTCCCGCTACCTGCCGCAGGACCAGCTCCAAGATTACCAGCA CTTTGTGAAGATGAAATCTGCTCTCATCATTGAGCAGAGAGAGCTGGAGGAGAAGATCAAACTTGGAGAA GAACAGCTTAAATGCCTCAAAGAGAGCCTGCACTTGGGGCCCAGCAATTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191730
Insert Size	4464 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191730.1, NP_001178659.1
RefSeq Size	4741 bp
RefSeq ORF	4464 bp
Locus ID	317391

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