Col5a2 (NM_053488) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217645 representing NM_053488 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCAAACTGGGTGGTAGCAAGACCTCTCCTCATTCTTTTTGTTTTATTAGGGTACTGTGTCTCAA TAAAAGCCCAGGAACAAGAGAATGATGACTATGATGAAGAAATAGCCTGTACACAACATGGCCAGATGTA TTTAAATAGAGACATTTGGAAACCTTCTCCATGCCAGATCTGTGTCTGTGACAATGGTGCTATCCTCTGT GACAAGATAGAGTGCCAAGAAGTGCTCAACTGTGCCAATCCTGTAACACCTCCTGGGGAGTGCTGCCCAG CGTGTCCACAGACATCTGGAGGTGGAGATACAAGTTTTGGTAGAGGAAGAAAGGTGAAAGGCTTACCAGG GTATTATGAAATTGTAACGGGCATACGTGGTCGTCCAGGACCTGCAGGCCCTCCAGGATCACAAGGACCA AGAGGGGACCGAGGCCCCAAAGGAAGACCTGGTCCTCGTGGTCCTCAGGGAATTGATGGAGAGCCAGGTG TTCCTGGTCAGCCAGGTGCTCCAGGGCCTCCTGGGCACCCCTCTCACCCAGGACCTGATGGCATGAGCAG GCCCTTTTCAGCTCAAATGGCTGGGTTGGATGAAAAATCTGGACTTGGAAGTCAAGTGGGACTCATGCCT GGCTCTGTGGGTCCTATTGGCCCAAGGGGACCTCAGGGTTTACAAGGACAGCAAGGTGGTGTGGGACCTG CAGGACCTCCTGGTGAGCCTGGCGAGCCTGGACCAATGGGTCCAATTGGTGCCCGTGGACCAGAAGGCCC ACCTGGGAAGCCTGGAGAAGATGGTGAACCTGGAAGAAATGGGAATACAGGTGAAGTGGGGTTCTCAGGA TCACCAGGTGCTCGAGGCTTTCCTGGTGCTCCTGGTCTCCCTGGCTTGAAAGGTCACAGAGGAAACAAAG GTCTTGAAGGCCCCAAAGGTGAAGTTGGAGCACCTGGTGCTAAGGGTGAAGCTGGCCCCACCGGTCCAAT GGGTGCTATGGGCCCTCTGGGACCAAGAGGAATGCCAGGAGAGAGGGGAAGACTTGGGCCACAGGGTGCT CCTGGACAACGAGGTGCTCATGGCATGCCTGGAAAACCTGGACCAATGGGTCCCCTTGGGATACCTGGCT CTTCTGGCTTTCCAGGAAATCCTGGAGTGAAGGGAGAAGCAGGTCCTACTGGGGCCCGAGGCCCTGAAGG ACCTCAGGGACAGAGAGGTGAAACTGGGCCACCTGGTCCTGCTGGCTCTCATGGCCTTCCTGGTGCGGTG GGGACTGATGGTACACCTGGCGCTAAAGGACCCACAGGCTCTCCTGGAACCTCTGGCCCTCCTGGCTTAG CAGGGCCCCCTGGGTCTCCTGGGCCTCAGGGCAGCACTGGACCTCAGGGAATTCGAGGACAACTGGGTGA TCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGCCCAAAAGGAGAACCAGGGCCACATGGTATTCAGGGT CCTATTGGTCCACCTGGAGAAGAAGGCAAAAGAGGTCCTCGAGGGGACCCAGGCACAGTTGGTCCTCCAG GACCAATGGGAGAAAGGGGTGCTCCTGGCAATCGTGGTTTTCCAGGTTCTGATGGTTTACCTGGACCAAA GGGTGCTCAGGGAGAGCGTGGCCCTATAGGTTCTTCAGGGCCTAAAGGAGGCCAGGGAGATCCAGGACGT CCAGGCGAACCTGGACTTCCAGGTGCTCGGGGCCTGACAGGAAATCCTGGTGTTCAAGGTCCTGAAGGAA AACTTGGGCCTTTGGGTGCTCCTGGCGAAGATGGCCGACCAGGTCCTCCAGGCTCCATTGGTATTAGAGG GCAGCCTGGAAGCATGGGCCTTCCAGGCCCCAAAGGTAGCAGTGGTGACCTTGGGAAACCAGGAGAAGCT GGGAATGCTGGCGTGCCTGGGCAAAGGGGGGCTCCTGGCAAAGACGGAGAAGTTGGTCCTTCAGGCCCCG TGGGACCTCCGGGCCTAGCTGGGGAAAGAGGAGAACAGGGACCTCCAGGTCCTACTGGCTTTCAGGGACT TCCTGGGCCTCCAGGGCCACCTGGAGAAGGTGGAAAGCCTGGTGATCAAGGTGTTCCTGGAGAGCCTGGA GCTGTTGGGCCATTAGGACCTCGGGGAGAAAGAGGGAATCCTGGAGAAAGAGGAGAGCCAGGGATAACTG GATTACCTGGGGAGAAAGGCATGGCTGGAGGACATGGCCCTGATGGTCCAAAGGGCAACCCAGGTCCAAC TGGGACCATTGGAGACACAGGGCCACCAGGTCTTCAAGGCATGCCAGGAGAAAGAGGAATTGCAGGGACA CCTGGTCCTAAGGGTGACAGAGGTGGCGTAGGAGAGAAAGGTGCTGAAGGAACAGCTGGAAATGATGGGG CAAGGGGTCTTCCTGGTTCCTTGGGACCTCCAGGTCCTGCAGGCCCTACTGGAGAAAAGGGTGAACCTGG TCCTAGAGGATTAGTTGGCCCTCCTGGCTCCCGTGGCAATCCTGGTTCTCGGGGGGAAAATGGTCCAACT GGAGCTGTTGGTTTTGCTGGACCCCAGGGTCCCGATGGACAGCCTGGTGTCAAGGGTGAGCCTGGAGAGC CAGGGCAGAAGGGAGATGCTGGGTCTCCTGGACCCCAAGGACTTGCAGGATCACCTGGCCCTCATGGTCC TCATGGTGTTCCTGGACTGAAAGGTGGTCGAGGAACCCAGGGTCCACCTGGTGCTACAGGATTTCCTGGT TCTGCTGGAAGAGTTGGGCCTCCAGGCCCCGCTGGAGCTCCAGGACCTGCAGGGCCCATAGGAGAACCTG GGAAGGAAGGACCACCCGGTCTTCGTGGGGACCCTGGCTCTCATGGGAGAGTGGGTGATAGAGGACCAGC TGGCCCACCTGGTAGCCCAGGAGACAAAGGGGACCCAGGGGAAGATGGACAACCTGGTCCAGATGGCCCC CCTGGTCCTGCTGGTACCACTGGGCAAAGAGGAATTGTTGGAATGCCTGGACAACGTGGGGAGCGAGGAA TGCCTGGCCTTCCTGGCCCAGCGGGTACACCAGGAAAAGTAGGACCGACTGGTGCAACAGGAGATAAAGG TCCCCCTGGACCCGTAGGACCTCCAGGTTCCAATGGTCCTGTGGGGGAACCTGGACCAGAAGGTCCGGCT GGTAATGATGGTACCCCAGGACGGGATGGTGCTGTTGGAGAACGGGGTGACCGTGGAGACCCCGGGCCTG CTGGTCTACCAGGTTCTCAGGGTGCCCCTGGGACCCCTGGCCCTGTCGGTGCACCAGGAGATGCAGGACA AAGAGGAGAGCCAGGTTCCCGAGGCCCTGTAGGACCACCTGGTCGAGCTGGAAAGCGTGGTTTACCTGGA CCACAAGGACCTCGAGGTGATAAGGGTGACAATGGAGACAGAGGTGACAGAGGTCAGAAAGGCCACAGAG GCTTCACTGGCTTGCAGGGTCTTCCTGGCCCTCCTGGTCCAAATGGTGAACAAGGAAGTGCTGGCATTCC TGGTCCTTTTGGCCCAAGAGGTCCTCCAGGACCAGTAGGTCCTTCAGGAAAAGAAGGAAACCCTGGGCTA CTTGGACCAATTGGACCTCCTGGTGTGCGGGGAAGTGTAGGAGAAGCAGGACCAGAGGGCCCTCCTGGTG AACCTGGCCCTCCTGGCCCTCCAGGTCCCCCTGGTCATCTTACTGCTGCTCTTGGTGATATCATGGGACA CTATGATGAGAACATGCCAGATCCACTTCCAGAATTTACAGAAGACCAGGCAGCTCCAGATGACACAAAC AAAACTGACCCTGGAGTCCATGCTACTCTGAAGTCTCTCAGTAGTCAAATTGAAACCATGCGGAGTCCTG ATGGTTCCAAGAAGCACCCAGCCCGCACTTGTGATGATTTAAAGCTTTGCCATCCTACAAAGCAGAGTGG TGAATATTGGATTGATCCTAACCAGGGATCAGCTGAAGACGCAATCAAAGTTTACTGCAATCTGGAGACA GGAGAGACATGCATTTCCGCAAACCCAGCCAGTGTTCCACGGAAAACGTGGTGGGCCAGCAAATCCCCTG ACAATAAGCCCGTGTGGTATGGTCTCGACATGAATAGAGGATCTCAGTTCACATATGGAGATTACAAGTC CCCTAACACAGCTATTACTCAGATGACCTTTCTGCGCCTTTTATCAAAAGAAGCCTCCCAGAACCTTACT TATATATGTAAGAACACTGTTGGTTATATGGATGATCAAACTAAAAACCTCAAGAAAGCTGTGGTTCTTA AAGGGTCAAATGACTTAGAAATAAAAGGAGAAGGAAACATCAGATTCAGATACACCGTTCTTCAAGATAC CTGTTCAAAGCGAAATGGAAATGTAGGCAAGACTGTCTTTGAATACAGAACACAGAATGTGGCCCGCTTG CCCATCATAGATATCGGTCCTGTGGATATTGGCAATGCAGACCAGGAATTTGGCTTTGAAATTGGGCCAG TTTGTTTCATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053488
Insert Size	4494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053488.1, NP_445940.1
RefSeq Size	6085 bp
RefSeq ORF	4494 bp
Locus ID	85250
Gene Summary	alpha chain of type V collagen; has a role in bone growth [RGD, Feb 2006]

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