Zfyve16 (NM_001191958) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564784
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217652 representing NM_001191958 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTACTTTAAAGCAGCTGTCAGTGACTTGGACAAACTTCTTGATGATTTTGAGCAGAACCCAG AAAAACAAGATTACTTCCAAGATGTGCAGGATGTTTATGCTTTTAACCAGTGTTCAGTTTCTTCAGAGTT GGCCTCTCCACAGCTAGCTTTACTGTCAAAGGACCAAAGATGCATTAGTAGTCATGCCTCATCAGAAACA TGCTGTGAAGCAAATGAGACTTTTCTCCAAGGAAAGATACCTGAGGGGTTAACCTCTAAACAGAATGAAA AAAACGTAGCGGGACTTGATCTTCTTTCTTCTGTGGATGCTAGTACTTCAGATGAGATCCAGCCTTCATG TATGAGACGATGTAGTAAGCCTGTCTGTGACCTGATAAGTGACATGGGTAACTTAGTACACGCCACCAGT AGTGAGGAAGACATTAAACAGTTATTGCCAGATGACTCGAAGTCTCCTGCAGATACTTTGATCACACTGG ACTCATCTTCAGTTTCAGAGGTTTTGATCGGATTGGATTTGTCTTCAGTTTCAGATACTCTCGCTGTTTC TTCAGTAGACGGTGGTAGTCAGGCTGTCAGGAAAGAGCAGGATACCAACGCTGGTGTGCAAAGCAGAGAT AACAGTAGGATCCAAGAATCGGGTGCAGAAGTGGACATGGCACTTTCTGATTCTTGTAAATATAGTAGAA CAGAAAATTTAAAGGATAAAATGATTTCTAATGAGTTAGAAACAGTTGATTTTGGCATGCCATCTGTTTT GACTGAACAGAGTTCAGAAATGTCCAATACCAAAGATAGCCCACAATACCAGAGGCTGCCATGTGAGCTG TTAAAAGATGACGGTGATTTGGCAGAAGAGAAAGTAGGCATGGCAGGCAATAGTACAAAGTGTTTAGAAG AAGGAGGTAACATAATTGCTATGCCTTGCAAGCTTCCAAAAAATGAAGAGATATCCCCAAATGACCCAAC TTCAAAAGATGAAAACTTCAAGTTACCTGACTTTTCTGTCCAGGAAAGTAGAACTGCTGTGTTCATAAAG CAGACTGTGGCAGAGGACTCGGTAAAGTCAGACCTTAAAGATAATAATGATGTCGTCCAAGTTTCTTCTT CATCTCTACATGTTTCGAGTGATGGTGTGTCCCCTTCACTGTCCTGTCTTTCATTGTCTGGGTCTTTGTG TGGATCCTTAGTTGACTGTAATGCACATGGTGATTTTTTACCCTCTGATGAGTCTAAAGGTCAGAACAAT GACGCAGTAACTGCACATGAGGAGATACAGAAGAGTGCCGCTTTAGATGGGGAGACAGATCTCCTGAAGA AGGAAAAGCAGGAAAGCATGTTTCTTCAGCCAGTAAATGATAAGGAAGGGGATGGAGAGGTTGAACTTGA GGAGATGGTAATCAGCGGTGAGCCTTTGGAGTACCCTGAGGGTACCAACCCTGCCGCAGCTGGCTCTCAA ATGGCACCCCCTACTGTGAGTGCCTCAGAGGCTCCTGGTCAGTGCGAAGCTCTCACTTTTTCCAGTGGCG ATATGGATGGGCAAGAGTTAGATTACTTTAATATTGATGAAAGCATGAGAAGTGGCATACTGGTTAGCGA TGCTGAACTTGATGCTTTTCTGAAGGAACAGAGTCTTCAGAACTCTAACACCATGTCTTTTGGAGAAAAT GCAAGTGATTCACAATTGCAGATGAATCAGATAGATATGAAAGGATTATGTGATGAAAATGCTGGTGATA GATACTTCAATGCTGAAGCAGGAGCTGCTGGCGAAAGTGGAGGCGTTGTGATTTGTGAAACAACTGATAA AGAAAACACAGTACAAAATGGTGGTCTTTCTGTAGGAGAAAAAAGCGCAATTCCAACGGAAAGAGGGTTA TCTGCTAGTCAGCCTGACATACGGAGTGAAGTGCCAGTCCCCAGTATTAACACCCAGGCTGTCGGAGGAG CTAGACCTAAGCAGCTGCCGAGTCGTCCACCAGGAACAAGCAGTTCACAGGGACCGAGTAAGCCACATGT TGTAGGTGTGCCAGCAGGCGAGCCAAGCACAGCAAATGCTGCTGCTGCACCCACTCGCCCTGCAGATCAT ACACCTGATTCTCAGGTTAGCTTCAACTCCAATTATATCGACATAGAAAGTAATTTCGAAGGTGGATCCA GTTTTGTAACAGCAAACAAGGATTCACTGCCTGAGAATATACGGAAAGAAGGCTTGGTTTTGGGCCAGAA ACAACCTACGTGGGTTCCTGATTCAGAAGCTCCAAACTGTATGAACTGCAAAGTCAAATTCACTTTTACA AAACGGCGACACCACTGCCGAGCTTGTGGGAAAGTATTTTGTGGTGTCTGTTGTAACAGGAAATGTAAGC TTCAGTATCTAGAAAAGGAAGCAAGAGTGTGTGTGATCTGCTATGAGACTATTAATAAAGCTCAGGCATT TGAAAGGATGATGAGTCCAGGTGGTGCTTGTCTTAAGTCTAATCATTCTAATGAGTGTGTCACTGCCCAG CCACTTCAGGAGACCCAAACGTCCAGCATACCTTCACCTACAGCTTTGCCCATCTCAGCACTGAAACAAC CAAATGTCGAAGGACCATGTTCCCGAGAACAGAAGAGAGTATGGTTTGCAGATGGCATACTGCCGAATGG TGAAGTTGCAGATACTACGAAATTATCCTCTGGAAGTAAAAGGTGTTCTGATGACTTTAGTCCTGTCTTA CCTGATGCGCCCACAGTGATAAATAAAGTGGACCATACCCCCTCTCCTATGGTGGAGAAAGCAAACAACA GTGTAGGAGACGTTCTAAGAAGTGAGATAACTCAGAGTCCGATTTGTCGTGCTGCACCTCTGGAAATACT GCCTGGGAGCACAGGCGCTGAAGGCTTGCCCACAGCTGGCCCTTTCACACTAGAAGATGATGTTTTTGTG GACAGTGAGGAGCCATCTACTCCTACTGTTGTCCCTGCTAATACTGGTTTGCCTGTTGCTAGTGTTTCAG ACTATAGGTTATTGTGTGGTGTTGCCAAATGTGTCTGCAATAACATTAGTCTTCTACCAGATGACGATGT TGGTTTGCCTCCCCTTCTGGCCACATCTGGAGAAGATGGATCAGTGCCTGTAGTACAAGAACATCCATCT CATGAGCAGATCATTTTGCTTCTTGAAAGTGAAGGATTTCCTCCTGCCACATTTGTTCTAAATGCAAACC TACTTGTGAATGTCAAGTTGGTATTTTATTCCTCAGATAAGTATTGGTACTTCTCAACCAATGGATTGCA TGGTTTGGGACAGGCAGAAATTATTATCCTGTTGCTATGCTTGCCAAATGAGGATACAGTTCCTAAGGAC ATCTTTAGACTATTCATCACCATATATAAGGATGCTTCGAAAGGGAAGTACGTAGAAAACTTGGACAATC TGACCTTTACTGAGAGTTTTCTCAGTAGCAAGGATCACGGAGGGTTCCTATTCATCACACCTACCTTTCA GAAACTTGACGATCTCCCAGTACCAAGGAGCCCTTTTCTCTGTGGCATTCTCATCCACAAGCTGGAGATT CCCTGGGCAAAGGCTTTCCCTATGCGTCTCATGTTGAGATTAGGCGCAGAATACAAAGCATATCCTGCTC CTCTAACAAGTGTCAGAGGCAGGAGACCGCTTTTCGGAGAAATAGGACATACTATTATGAACTTACTTGT TGATCTTCGAAATTACCAGTATACATTGCATAACATCGATCAGCTGTTAATCCATATGGAAATGGGGAAA AGCTGTATAAAGATACCTCGGAAAAAGTACAGTGATGTATTGAAGGTAATAAATTCTTCTAACGAGCACG TCATCAGTATTGGGGCTAGTTTTAGTACAGAAGCAGATTCTCATCTGGTCTGTGTACAGAGTGATGGAGT TTATCAGACTCAGGCCAACAGTGCCACTGGTCAGCCAAGGAAAGTGACAGGTGCAAGTTTTGTGGTGTTC AATGGTGCTCTGAAAACATCTTCAGGATTTCTTGCCAAATCCAGCATAGTCGAAGATGGCTTAATGGTTC AAATAACCCCAGAGACCATGGATGGCTTACGGCTGGCTCTACGAGAGCAAAAGGACTTTAAAATTCAGTG TGGAAAAGTTGATGCAGTAGACCTGAGAGAATATGTGGACATCTGTTGGGTGGATTCGGAAGAAAGGAAA AACAAAGGTGTTATCAGTTCAGTGGATGGGATGTCATTAGAAGGATTTCCAAGTGAAAAAATAAAACTGG AAACAGATTTTGAAACTGAAGAGAAGACTGTGAAATGCACTGAGGTGTTCTGCTTCCTAAAGGACCAGGA TATCTCTCTCTCCTCGAGTAGTTACCAGTTTGCAAAAGAGATCGCCACGGCTTGCAGTGCGGCCCTGTGC CCTCACCTGAGGACTCTAAAGAGTAACAGAATGAACAGAATTGGACTCAGAGTTTCTGTTGACACTGATA TGGTTGAATTTCAGGCAGGCTGTGAAGGGCAGCTTCTGCCTCAGCATTACCTAAACGATCTTGATAGCGC TCTGATACCTGTGATCCACGGCGGGACCTCCAACTCGACCGTGCCACTCGAGATAGAGTTAGCATTCTTC ATTTTAGAAAACCTTCTGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191958
Insert Size	4644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191958.1, NP_001178887.1
RefSeq Size	5372 bp
RefSeq ORF	4644 bp
Locus ID	499508

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